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EC number: 476-720-8 | CAS number: 768-35-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 06 Jul 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- At the start of the exposure and at the renewal (24 and 48 h), samples of the freshly prepared test item concentrations were taken and analyzed. At renewal and at the end of the exposure (24 and 48 h), sampled of the 24 h old media were taken directly fron the test vessels and analyzed.
- Vehicle:
- no
- Details on test solutions:
- Stock solution:
A stock solution (100 mg/L of the test item were weighed out) was freshly prepared with dilution water prior to the start of the exposure (0 h) and prior to the renewal of the test solutions (24 h). The stock solution was mixed thoroughly by manual agitation.
Test concentrations:
5 test item concentrations in a geometirc series with a separation factor of 2, prepared by dilution of the stock solution of 100 mg/L with dilution water were tested as follows: 6.25, 12.5, 25.0, 50.0 and 100 mg/L.
Control:
Dilution water without test item incubated under the same semi-static conditions as the test group. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna STRAUS (Clone 5)
- Source: Institut für Wasser-, Boden und Lufthygiene (WaBoLu), Berlin, Germany
- Age: < 24 h
FEEDING DURING TEST
- Food type: 5 x per week ad libitum with a mix of unicellular green algae - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 0 h: 171 mg CaCO3/L
24 h: 173 mg CaCO3/L - Test temperature:
- 20.2 - 20.4 °C
- pH:
- 0 h: 7.42
24 h: 7.44 - Dissolved oxygen:
- 0 h: 8.88 mg/L
24 h: 8.96 mg/L - Conductivity:
- 0 h: 437 µS/cm
24 h: 428 µS/cm - Nominal and measured concentrations:
- Nominal concentrations: 6.25 - 12.5 - 25.0 - 50.0 - 100 mg/L
Mean measured concentrations:6.68 - 13.1 - 25.9 - 52.1 - 103.5 mg/L (details see Table 2 below) - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers (4 (ID) x 7 (H) cm)
- Type: loosely covered with watch glasses
- Material, size, fill volume: Glass beakers, 50 mL capacity, 20 mL
- Aeration: no
- Renewal rate of test solution: The test solutions were renewed after 24 h. For this purpose, a second set of test vessels was filled with the freshly prepared test solutions and the daphnids were transferred by pipette.
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Preparation of dilution water: Culture Medium Elendt M4 according to OECD 202, Annex 3 (2004)
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16/8 hours light/dark cycle
- Light intensity: Diffuse light, light intensity of max. 1500 Iux
EFFECT PARAMETERS MEASURED
Immobilization was determined in all groups after 24 and 48 hours. An animal was considered immobile, if it was not able to swim in the water phase within 15 seconds after gentle agitation of the test vessel.
RANGE-FINDING STUDY
- Test concentrations: 1, 10, 100 mg/L
- Results used to determine the conditions for the definitive study: yes, EC50 between 10 and 100 mg/L - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 35.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 50.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC10
- Effect conc.:
- 26.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- The study was performed according to OECD Guideline 202 (2004). The validity criteria were fulfilled:
• In the control group, no daphnids were immobilized or showed any signs of disease or stress, e.g. discoloration or unusual behavior such as trapping on the surface of the water, during the 48-hour test period (required: not more than 10% of the daphnids immobilized in the control).
• The dissolved O2 concentration in the 24-hours old media at the end of the exposure intervals was > 7.97 mg/L (required: a 3 mg/L at the test end of the exposure intervals) in the test vessels of all tested concentration levels and the control. - Results with reference substance (positive control):
- The percentage of immobility for the reference item potassium dichromate (SIGMA-ALDRICH, batch number MKBV0900V, purity 99.0%, CAS RN 7778-50-9) was determined after 24 hours from 2017-07-11 to 2017-07-12. The EC50 value was determined to be 1.31 mg/L, which is in the valid range (0.6 - 2.4 mg/L)
- Reported statistics and error estimates:
- The EC10- and the EC50-values after 24 hours were calculated by sigmoidal dose-response regression with the software GraphPad Prism5. The respective 95% confidence limits were calculated from the standard error and the t-distribution. All calculations were carried out from the best-fit values with the software GraphPad Prism5. The EC10- and the EC50-values after 48 hours were calculated by linear dose-response regression with the software GraphPad PrismS. Since only 0 and 100% immobilization occurred, only the highest test item concentration resulting in 0% immobilization and the lowest concentration level resulting in 100% immobilization were used for calculation of the EC10 and the EC50 and as their 95% confidence limits.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the nominal concentrations of the test item, the 48 h EC50 for Daphnia magna was 35.4 mg/L (95% confidence limits: 25.0 - 50.0 mg/L).
- Executive summary:
In the acute immobilization test with Daphnia magna (STRAUS), the effects of the test item were determined at the test facility according to OECD 202 (2004). The study was conducted under semi-static conditions over a period of 48 hours with 5 concentrations of the test item in the range of 6.25 to 100 mg/L, prepared in a geometric series with a separation factor of 2. All tested concentration levels were visually clear throughout the exposure. Twenty daphnids (divided into 4 replicates with 5 daphnids each) were exposed to each concentration level and the control. The concentrations of the test item were analytically verified via HPLC-DAD in fresh media of all concentration levels and in the control at the start of the exposure and at renewal (0 and 24 hours) and in 24-hours old media at renewal and at the end of the test (24 and 48 hours). The measured concentrations of the test item in fresh media at the start of the exposure and at renewal (0 and 24 hours) were in the range of 101 to 109% of the nominal values, and 100 to 111% in old media at renewal and at the end of the test (24 and 48 hours). The measured test item concentrations were all within ± 20% of the nominal concentrations. This indicates that the test item concentrations were successfully maintained for the duration of the test. Therefore, all effect concentrations are based on the nominal concentrations of the test item. The validity criteria of the test guidelines were fulfilled. The 48 h EC50 for Daphnia magna was 35.4 mg/L (95% confidence limits: 25.0 - 50.0 mg/L), the EC10 was 26.8 mg/L.
Reference
Table 1: Immobilization rates after 24 and 48 h
Nominal test item concentration [mg/L] |
IMMOBILIZATION [%] |
|||||||||
24 hours |
48 hours |
|||||||||
Replicates |
Replicates |
|||||||||
1 |
2 |
3 |
4 |
MV |
1 |
2 |
3 |
4 |
MV |
|
100 |
100 |
100 |
100 |
100 |
100 |
100% mortality after 24 hours |
||||
50.0 |
60 |
40 |
40 |
40 |
45 |
100 |
100 |
100 |
100 |
100 |
25.0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
12.5 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
6.25 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
Control |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
Table 2: Measured Concentrations and Percent of Nominal Concentration of the Test Item
Sampling date |
2017-07-05 Fresh media, 0 hours |
2017-07-06 Old media, 24 hours |
2017-07-06 Fresh media, 24 hours |
2017-07-07 Old media, 48 hours |
||||
Start of analysis |
2017-07-05 |
2017-07-06 |
2017-07-06 |
2017-07-07 |
||||
Nominal test item concentration [mg/L] |
Art. 281382 (3-Fluoro-phenylboronic acid) |
|||||||
Meas. conc. [mg/L] |
% |
Meas. conc. [mg/L] |
% |
Meas. conc. [mg/L] |
% |
Meas. conc. [mg/L] |
% |
|
100 |
103 |
103 |
104 |
104 |
Not determined, due to 100% immobilization after 24 hours |
|||
50.0 |
51.1 |
102 |
49.9 |
100 |
51.7 |
103 |
55.6 |
111 |
25.0 |
25.4 |
101 |
26.1 |
104 |
25.7 |
103 |
26.6 |
106 |
12.5 |
12.6 |
101 |
12.8 |
102 |
13.4 |
107 |
13.7 |
109 |
6.25 |
6.48 |
104 |
6.49 |
104 |
6.84 |
109 |
6.91 |
111 |
Control |
< LOQ |
< LOQ |
< LOQ |
< LOQ |
Meas. conc. = measured concentration of the test item, mean value of 2 injections, dilution factors taken into account
% = percent of the nominal concentration of the test item
LOQ = limit of quantification of the analytical method (0.1 mg/L of the test item)
Description of key information
Based on the nominal concentrations of the test item, the 48 h EC50 for Daphnia magna was 35.4 mg/L (95% confidence limits: 25.0 - 50.0 mg/L) (reference 6.1.3-1).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 35.4 mg/L
Additional information
In the acute immobilization test with Daphnia magna (STRAUS), the effects of the test item were determined at the test facility according to OECD 202 (2004). The study was conducted under semi-static conditions over a period of 48 hours with 5 concentrations of the test item in the range of 6.25 to 100 mg/L, prepared in a geometric series with a separation factor of 2. All tested concentration levels were visually clear throughout the exposure. Twenty daphnids (divided into 4 replicates with 5 daphnids each) were exposed to each concentration level and the control. The concentrations of the test item were analytically verified via HPLC-DAD in fresh media of all concentration levels and in the control at the start of the exposure and at renewal (0 and 24 hours) and in 24-hours old media at renewal and at the end of the test (24 and 48 hours). The measured concentrations of the test item in fresh media at the start of the exposure and at renewal (0 and 24 hours) were in the range of 101 to 109% of the nominal values, and 100 to 111% in old media at renewal and at the end of the test (24 and 48 hours). The measured test item concentrations were all within ± 20% of the nominal concentrations. This indicates that the test item concentrations were successfully maintained for the duration of the test. Therefore, all effect concentrations are based on the nominal concentrations of the test item. The validity criteria of the test guidelines were fulfilled. The 48 h EC50 for Daphnia magna was 35.4 mg/L (95% confidence limits: 25.0 - 50.0 mg/L), the EC10 was 26.8 mg/L (reference 6.1.3-1).
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