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EC number: 222-904-6 | CAS number: 3658-48-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 19 Oct - 17 Nov 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Version / remarks:
- July 1992
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Landesanstalt für Umwelt, Messungen und Naturschutz Baden-Württemberg, Karlsruhe, Germany
Test material
- Reference substance name:
- Bis(2-ethylhexyl) phosphonate
- EC Number:
- 222-904-6
- EC Name:
- Bis(2-ethylhexyl) phosphonate
- Cas Number:
- 3658-48-8
- Molecular formula:
- C16H35O3P
- IUPAC Name:
- bis(2-ethylhexyl) phosphonate
- Test material form:
- liquid
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: AZV Staufener Bucht, sampled on 17 Oct 2017
- Storage length: 1 day between sampling and start of incubation of the test item
- Preparation of inoculum for exposure: drying at 105 °C for 5 hours, washed twice with tap water by settling the sludge, decanting the supernatant and re-suspending the sludge
- Concentration of sludge: 30 mg dry solids/L - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 20 mg/L
- Based on:
- TOC
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: according to guideline
- Test temperature: required range of 20.6 – 24.0 °C.
- Aeration of dilution water: tolerated range of 1.6 – 5.5 bubbles/second (counted bubbles: 2.4 – 5.1 bubbles/second)
- Suspended solids concentration: 30 mg dry solids/L
TEST SYSTEM
- Culturing apparatus: Gas wash bottles (2000 mL volume)
- Number of culture flasks/concentration: 3
- Method used to create aerobic conditions: The CO2-free air production system consists of an air compressor, three 1000 mL gas wash bottles filled with dry soda lime in series followed by one bottle filled with 0.1 M NaOH (sodium hydroxide). At the end of the system is one gas wash bottle filled with demineralised water, followed by an empty one to catch any drops of condensation water. A color change of the soda lime from white to blue indicates that the CO2 absorption capacity is depleted. The CO2-free air is passed on to an air distributor with two input and 22 output channels and through PE-tubes.
- Measuring equipment/method: Degradation is followed by determining the carbon dioxide produced and absorbed to sodium hydroxide via IC-measurement (IC = inorganic carbon). Degradation is followed by determining the carbon dioxide produced and absorbed to sodium hydroxide via measurement of the inorganic carbon (IC). IC measurement was performed with a total carbon analyser (TOC-L Shimadzu) by purging the inorganic carbon with H3PO4 (25%) using a nondispersive infrared (NDIR) detector.
SAMPLING
- Sampling frequency: Sampling for IC measurement took place on days 0, 4, 7, 11, 14, 21, 28 and 29.
- Sampling method: Sampling was performed through the lateral connecting pieces through the butyl rubber septum using 5 mL PE syringes.
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes, three bottles
- Toxicity control: yes, one bottle
- Reference substance: yes, three bottles
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradation
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 82.2
- Sampling time:
- 28 d
- Details on results:
- The test item reached the criteria for ready biodegradability according to the OECD criteria (60% of ThCO2 within a 10-d window).
The degradation extent in the toxicity control was 61.2% within 14 days. The test material is therefore considered to have no inhibitory effect on the inoculum according to the criterion of the guideline.
For details on biodegradation rates, please refer to Table 1 in section "Any other information on results incl. tables".
BOD5 / COD results
- Results with reference substance:
- The reference substance reached the pass level for ready biodegradability within 4 days. The degradation after 28 days was 89.5-97.8%.
Any other information on results incl. tables
Table 1: Ultimate biodegradation after x days [% of ThCO2]
Reactor |
Days |
||||||||
0 |
4 |
7 |
11 |
14 |
21 |
28 |
29 |
||
7 |
Test flasks |
0 |
6.7 |
25.0 |
56.3 |
67.6 |
86.2 |
77.2 |
77.5 |
8 |
0 |
8.1 |
39.7 |
64.0 |
70.2 |
93.0 |
89.9 |
88.9 |
|
9 |
0 |
7.8 |
29.1 |
55.1 |
63.3 |
81.0 |
79.4 |
80.3 |
|
4 |
Reference flasks |
0 |
68.7 |
84.1 |
86.3 |
87.3 |
101.4 |
89.5 |
89.9 |
5 |
0 |
80.9 |
89.9 |
92.2 |
92.2 |
106.6 |
97.8 |
99.3 |
|
6 |
0 |
73.9 |
86.4 |
86.3 |
88.9 |
107.2 |
96.3 |
98.3 |
|
10 |
Toxicity control (Test item + Reference item) |
0 |
37.3 |
45.8 |
53.9 |
61.2 |
84.4 |
77.9 |
78.8 |
Table 2: Validity criteria
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
Difference of extremes of replicate values of the removal of the test chemical at the plateau, at the end of the test or at the end of the 10-d window, as appropriate, is less than 20%. |
< 20% |
yes |
Percentage degradation of the reference compound has reached the pass levels by day 14. |
> 87% |
yes |
The toxicity control should degrade to at least 35% (based on DOC) or at least 25% (based on ThOD or ThCO2) within 14 d. |
> 61% |
yes |
The IC content of the test substance suspension in the mineral medium at the beginning of the test must be less than 5% of the TC. |
< 5% |
yes |
The total CO2 evolution in the inoculum blank at the end of the test should not normally exceed 40 mg/L medium. |
29.9 mg/L |
yes |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- For further details please refer to "Any other information on results incl. tables."
- Interpretation of results:
- readily biodegradable
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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