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EC number: 220-668-9 | CAS number: 2855-27-8
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- freshwater
Additional information
Biodegradation of the test substance has been tested according to the draft OECD guideline 301B. After 28 days a biodegradation rate of 11% with a test substance concentration of 10 mg/L has been observed. A test substance concentration of 20 mg/L resulted in a biodegradation rate of 1%. Therefore, the test substance is not readily biodgradable. However, it is unclear if the test substance is still present in the test medium during the test duration. Due to the high Henry's Law constant and a low water solubilty, a high volatility of the test substance from aquatic systems is expected. The test substance might not have been bioavailable in the test system.
In a second test, the ready biodegradability of the test substance has been examined with a non-adapted activated sludge in the Headspace Test according to OECD 310. In the study, silicone oil has been used to achieve an improved bioavailability of the test substance and also the controls has been tested in presence of silicone oil. For a prolongation of the study period from 28 days to 56 days additional replicates of the test substance and the inocolum control have been used. The biodegradation has been followed by TIC analyses of the quantity of CO2 produced by the respiration of bacteria during the study period. The degradation rate of the reference substance (with and without silicone oil) reached the pass level of 60% within 7 days and a maximum of 89% after 28 days. The toxicity control revealed no inhibition of the biodegradation of the reference substance by the test substance. The test substance replicates have not reach the 10% level (beginning of biodegradation) until test end. No biodegradation has been observed after 28 days and 56 days. The test substance has been classified as not readily biodegradable under the tested conditions.
A further prediction with the QSAR program Biowin, revealed ambiguous results. The models Biowin 1 and Biowin 2 predicted a fast biodegradation. The estimated biodegradation time is weeks or days to weeks according to Biowin model 3 and 4, respectively. Biowin model 5 and 6 predicted no fast biodegradation. The overall conclusion of the biowin model is no ready biodegradation.
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