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EC number: 216-323-7 | CAS number: 1559-35-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- April 24, 2017 to June 27, 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: TXEG
- Expiration date of the lot/batch: December 13, 2017
- Purity test date: December 13, 2016
RADIOLABELLING INFORMATION (if applicable)
- Radiochemical purity:
- Specific activity:
- Locations of the label:
- Expiration date of radiochemical substance:
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: 20 +/- 5 degrees celsius in the dark
- Stability under test conditions:
- Solubility and stability of the test substance in the solvent/vehicle:
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium:
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing:
- Preliminary purification step (if any):
- Final dilution of a dissolved solid, stock liquid or gel:
- Final preparation of a solid:
FORM AS APPLIED IN THE TEST (if different from that of starting material)
TYPE OF BIOCIDE/PESTICIDE FORMULATION (if applicable)
OTHER SPECIFICS: - Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: all test concentrations and the water control
- Sampling method: one sample from the test medial of all test concentrations and the control were collected at the end of the test (after 96 hours) from the approximate center of the aquaria
- Sample storage conditions before analysis: = 20 degrees Celsius - Vehicle:
- no
- Details on test solutions:
- A stock solution of 1000 mg test item/L was prepared by dissolving 2000.2 mg test item into 2000.2 mL test water by intense stirring for 40 minutes and ultrasonic treatment for 5 minutes. Adequate volumes of this stock solution were diluted with test water to prepare the test media of the desired test concentrations.
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: Rainbow Trout
- Strain:
- Source: Forellenzuchtbetrieb Stork, 88348 Bad Saulgau, Germany
- Age at study initiation (mean and range, SD): juveniles,
- Length at study initiation (length definition, mean, range and SD): 4.65 cm +/- 0.3 cm
- Weight at study initiation (mean and range, SD): 0.88 g +/- 0.1 g
- Method of breeding:
- Maintenance of the brood fish:
ACCLIMATION
- Acclimation period: 12 days
- Acclimation conditions (same as test or not): same as test
- Type and amount of food during acclimation:
- Feeding frequency during acclimation: 3x per week
- Health during acclimation (any mortality observed): mortality below 5%
QUARANTINE (wild caught)
- Duration:
- Health/mortality:
FEEDING DURING TEST
- Food type:
- Amount:
- Frequency: - Test type:
- static
- Water media type:
- freshwater
- Remarks:
- reconstituted water (ISO Method)
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- none
- Hardness:
- 25 mmol/L (250 mg/L) as CaCO3
- Test temperature:
- 15 to 15 degrees Celsius
- pH:
- 7.8 to 8.0
- Dissolved oxygen:
- 98 to 101% of air saturation
- Salinity:
- not applicable
- Conductivity:
- = 10 uS/cm
- Nominal and measured concentrations:
- Nominal test concetrations: 100, 45.5, 20.7, 9.4 and 4.3 mg test item/L and a control
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 12 litre glass aquaria
- Material, size, headspace, fill volume: 10 kitre test medium
- Aeration: yes
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): not applicable
- Biomass loading rate: < 1g fish/L water
- Range finding study : yes
- Test concentrations: 100, 45.5, 20.7, 9.4, and 4.3 mg/L - Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 67.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- no abnormal behaviors were observed and no mortality was reported in the control
- Reported statistics and error estimates:
- The LC50, LC20 and LC10 at the observation times were calculated by Probit analysis. The NOEC, the LOEC, the LC0 and the LC100 were determined directly from the raw data. The software used to perform the statistical analysis was ToxRat Professional, Version 3.2.1, ToxRat Solutions GmbH.
- Validity criteria fulfilled:
- yes
- Conclusions:
- No mortality was seen in the control vessel. The 96-hour LC50 value was determined to be 67.3 mg/L, with a no observed effect concentration at 20.7 mg/L.
- Executive summary:
The toxic effect of the test item Eastman ™ EEH Solvent to Rainbow Trout (Oncorhynchus mykiss) was assessed in a static concentration response test. Based on the test results the 96-hour LC50 was determined to be 67.3 mg test item/L based on nominal concentrations. The NOEC was determined to be 20.7 mg test item/L also based on nominal concentrations. The quantification of the test item Eastman ™ EEH Solvent in the test samples was performed using gas chromatography with flame ionization detection. At the start of the test 90% of the nominal test concentrations were found (average of the nominal concentrations of 100, 45.5 and 20.7 mg test item/L). After 96 hours test duration, 93% of the nominal value was determined (average of the nominal concentrations of 100, 45.5 and 20.7 mg test item/L). During the test the fish were exposed to a mean of 92% of nominal. The nominal concentrations of 9.4 and 4.3 mg test item/L were below the Limit of Quantification (LOQ). As these concentrations were below the 96-hour NOEC, dose verification was not required according to the study plan. Since the nominal concentrations of 100, 45.5 and 20.7 mg test item/L were within 100±20% recovery at test start and test end, all biological results were calculated based on nominal concentrations.
Reference
Description of key information
The toxic effect of the test item Eastman ™ EEH Solvent to Rainbow Trout (Oncorhynchus mykiss) was assessed in a static concentration response test. Based on the test results the 96-hour LC50 was determined to be 67.3 mg test item/L based on nominal concentrations. The NOEC was determined to be 20.7 mg test item/L also based on nominal concentrations.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 67.3 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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