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EC number: 255-785-4 | CAS number: 42373-04-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1989-05-04 to 1989-05-11
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1989
Materials and methods
Test guidelineopen allclose all
- Guideline:
- other: Short Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms
- Version / remarks:
- USEPA, 1985 (EPA/600/4-85/014)
- Guideline:
- other: North Carolina Ceriodaphnia Chronic Effluent Bioassay Procedure
- Version / remarks:
- December 1985, revised June 1988, North Carolina Department of Natural Resources and Community Development, Division of Environmental Management, Water Quality Section
- Principles of method if other than guideline:
- - Principle of test: the test item is diluted 5:1 with spring water and treated with sludge from City of Columbis WWTP in 1:4 ratio. Solution is aerated for 24 hours and filtered before tested. In an additional test the sludge without any test item is tested for toxicity.
- Parameters analysed / observed: mortality, offspring - GLP compliance:
- no
Test material
- Reference substance name:
- 3-methyl-2-[(1-methyl-2-phenyl-1H-indol-3-yl)azo]thiazolium chloride
- EC Number:
- 255-785-4
- EC Name:
- 3-methyl-2-[(1-methyl-2-phenyl-1H-indol-3-yl)azo]thiazolium chloride
- Cas Number:
- 42373-04-6
- Molecular formula:
- C19H17N4S.Cl
- IUPAC Name:
- 3-methyl-2-[(1-methyl-2-phenyl-1H-indol-3-yl)azo]thiazolium chloride
- Test material form:
- solid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Dye Preparation: 30 mg/L dye diluted 5:1 with spring water and treated with sludge from City of Columbis WWTP in 1:4 ratio. Solution aerated for 24 hours and the solution filtered before tested.
- Controls: one control 100% spring water, additional test for control of toxicity of sludge
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): stock solution was filtrated before using for dilutions
Test organisms
- Test organisms (species):
- Ceriodaphnia sp.
- Details on test organisms:
- TEST ORGANISM
- Age at test start: 2 to 24 hours
- Feeding during test: yes
- Food type: green alga Selenastrum capricornutum
- Amount: at rate of approx. 2000000 cells/mL per day
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 7 d
- Remarks on exposure duration:
- test solutions were renewed on days 2 and 5 with reproduction counts made at that time.
Test conditions
- Test temperature:
- 25 +/- 1°C
- pH:
- 6.92 - 7.60
- Dissolved oxygen:
- 6.50 - 8.50 ppm
- Nominal and measured concentrations:
- control, and dilutions of 5, 15, 30 and 50% of the stock solution
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 1ounce filled with 15 mL of solution
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
OTHER TEST CONDITIONS
- Photoperiod: 16h light/ 8h darkness - Reference substance (positive control):
- not specified
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 50 other: %
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Remarks on result:
- other: corresponding to 2.25 mg/L
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 50 other: %
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- Sludge control: the test was performed in the same way as the test with test item.
Concentrations of sludge (without test item): 5, 15, 30, 50% and a control.
Result: sludge is not chronically toxic to Ceriodaphnia at the tested concentrations.
Any other information on results incl. tables
Results of the main test
Concentration of the test item | Average Offspring | Mortality [%] |
Control | 15 | 10 |
5% | 19 | 0 |
15% | 20 | 0 |
30% | 17 | 10 |
50% | 15 | 10 |
Results of the sludge control test
Concentration of sludge | Average Offspring | Mortality [%] |
Control | 15 | 10 |
5% | 16 | 0 |
15% | 20 | 0 |
30% | 19 | 0 |
50% | 26 | 0 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- Mortality in negative control 10%, average # offspring = 19
- Conclusions:
- The 7-d NOEC (reproduction) to Ceriodaphnia sp. was determined at 2.25 mg/L nominal.
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