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Diss Factsheets
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EC number: 297-025-4 | CAS number: 93281-13-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From November 6 to December 4, 1990
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
- Version / remarks:
- adopted in 1981
- Deviations:
- yes
- Remarks:
- not impairing the study results (details below)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-A (Determination of the "Ready" Biodegradability - Dissolved Organic Carbon (DOC) Die-Away Test)
- Principles of method if other than guideline:
- Internal test method: SOP: MBA 001 02
- GLP compliance:
- yes
- Oxygen conditions:
- not specified
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- - Source of inoculum: effluent of a domestic treatment plan (ARA-Rein).
- Inoculation: mixture of polyvalent bacteria collected on 06.11.90.
- Initial cell/biomass concentration: 7 x 10^6/ml. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 40 mg/L
- Based on:
- DOC
- Remarks:
- nominal
- Details on study design:
- TEST CONDITIONS
- Composition of medium: according to the specification of the EEC Annex V, part C.4 resp. OECD 301 A.
- Test temperature: 23 ± 1 °C.
- Lighting: indirect daylight.
TEST SYSTEM
- Number of culture flasks/concentration: 3 flasks per concentration (only one concentration was tested).
- Estimation technique: TOC/DOC Shimadzu TOC-500.
CONTROL AND BLANK SYSTEM
- Inoculum blank: only inoculum, one replicate.
- Abiotic sterile control: test medium, without inoculum, one replicate.
- Toxicity control: test material and glucose, one replicate. - Reference substance:
- other: glucose
- Parameter:
- % degradation (DOC removal)
- Value:
- 0
- Sampling time:
- 28 d
- Details on results:
- Test item is not readily biodegradable.
From the results of the inhibition control, no inhibition on the activity of the bacteria can be found. - Results with reference substance:
- Biodegradation (28 d): 100 % based on DOC removal.
- Validity criteria fulfilled:
- not specified
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The substance is not readilly biodegradable.
- Executive summary:
The biodegradability of the substance was assessed by using an effluent of a domestic sewage tretment plant for 28 days, according to the EU Method C.4 - A and the OECD 301 A. The biodegradation was assessed in terms of elimination of Dissolved Organic Carbon (DOC). For this purpose, the DOC of the solution was measured at fixed time intervals (0, 3, 7, 14 and 28 days). The DOC was assessed in flasks containing the reference substance (glucose), while the inhibitory action of the substance was also evaluated. The amount of dissolved organic carbon removed from the test solutions was corrected for that derived from the blank inoculum.
Biodegradation (28 d): 0 % based on DOC removal.
Biodegradation (28 d): 100 % based on DOC removal.
Conclusion
The substance is not readily biodegradable.
Reference
Carbon determination | Flask | theor.conc mg/l |
DOC- concentration afte (mg/l) | ||||
Culture medium (nutrient solution..) |
0 (Co) | 3d | 7d | 14d | 28d | ||
with test material, with inocolum (Co,Ct) |
1 | 40 | 47.9 | 44.8 | 44.9 | 45.0 | 43.2 |
with test material, with inocolum (Co,Ct) |
2 | 40 | 47.4 | 44.9 | 43.9 | 44.6 | 42.3 |
with test material, with inocolum (Co,Ct) |
3 | 40 | 47.5 | 46.3 | 44.1 | 43.6 | 42.3 |
with test medium, without inocolum (sterile control) | 4 | 40 | 48.6 | - | - | - | 43.5 |
without test material, with glucose, with inocolum (glucose control) | 5 | 40 | 46.3 | 9.1 | 8.7 | 0 | 0 |
with test material, with inocolum, with glucose (inhibitory action) |
6 | 80 | 87.3 | 47.2 | 50.0 | 47.5 | 45.9 |
with inocolum (inocolum control ≈ Coi,Cti) |
7 | - | 10.2 | 6.2 | 5.5 | 0 | 0 |
Calculation | DOC- concentration after (mg/l) | ||||
Flask | 3d | 7d | 14d | 28d | |
% DOC removal (corrected by the black control) Dt =[((Co-Coi)-(Ct-Cti))/(Co-Coi)]*100 |
1 | 0 | 0 | 0 | 0 |
2 | 0 | 0 | 0 | 0 | |
3 | 0 | 0 | 0 | 0 | |
4 | 0 | 0 | 0 | 0 | |
5 | 92.0 | 91.1 | 100 | 100 | |
6 | 46.8 | 42.3 | 38.4 | 40.5 |
Description of key information
Not readily biodegradable
Key value for chemical safety assessment
Additional information
The biodegradability of the substance was assessed, according to the EU Method C.4 - A and the OECD 301 A. The biodegradation was assessed in terms of elimination of Dissolved Organic Carbon (DOC). Test item resulted as not readily biodegradable, i.e. 0 % of degradation after 28 days, based on DOC removal. From the results of the inhibition control, no inhibition on the activity of the bacteria can be found.
The results of the key study are supported and confirmed also by the Biochemical Oxygen Demand (BOD) test assessed after 5 days. The test item BOD 5 resulted to be 0 mg/O2 g, confirming the conclusion that the substance is not readily biodegradable.
The substance is a polyazo dye, derivative; it is not expected to be ready biodegradable because of its chemical structure and its specific function. Commonly, dyes undergo a primary transformation, i.e. discolourization due to the interrupting the conjugation. Nevertheless, the degradation process involves more steps and take more time.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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