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EC number: 261-118-8 | CAS number: 58096-47-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 14.03.2018 to 22.03.2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- not specified
- GLP compliance:
- yes
Test material
- Reference substance name:
- Decahydro-1,1,7-trimethyl-3a,7-methano-3aH-cyclopentacyclooct-3-yl formate
- EC Number:
- 261-118-8
- EC Name:
- Decahydro-1,1,7-trimethyl-3a,7-methano-3aH-cyclopentacyclooct-3-yl formate
- Cas Number:
- 58096-47-2
- Molecular formula:
- C16H26O2
- IUPAC Name:
- decahydro-1,1,7-trimethyl-3a,7-methano-3aH-cyclopentacyclooct-3-yl formate
- Test material form:
- liquid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- In the range finding test, Neonates of daphnia were tested for immobility in test concentrations of Clovanyl-3-Formate (CARYOLAN) at 0.01, 0.1, 1, 10 and 100 mg/L along with a negative control.
In the definitive test, Neonates of daphnia were tested for immobility in test concentrations of 0.4, 0.88, 1.94, 4.27, 9.39, 20.66, 45.46 and 100 mg/L (factor of 2.2)
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- One day prior to the start of the treatment, about 100 daphnia during range finding test and definitive test were incubated in approximately 2 L of test medium. The following day, the young ones hatched which were aged less than 24 hour old were collected and used in the test.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 2.39 mmoles/L (Total harness as CaCO3)
- Test temperature:
- 20.2 to 20.5
- pH:
- 7.7 to 7.8
- Dissolved oxygen:
- 7.2 to 7.4
- Salinity:
- Not mentioned
- Conductivity:
- <5 µS/cm
- Nominal and measured concentrations:
- Nominal concentrations 0.4, 0.88, 1.94, 4.27, 9.39, 20.66, 45.46 and 100 mg/L
- Reference substance (positive control):
- yes
Results and discussion
Effect concentrations
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 3.27 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- There was no immobilization of daphnia in the negative control and at the lowest concentration of 0.4 mg/mL either at 24 or at 48 hours post-exposure. The immobilization of daphnia was 5, 20, 30, 50, 85, 95, and 100 % at 24 hours and 15, 45, 50, 70, 100, 100 and 100 % at 48 hours post-exposure at the tested concentrations of 0.88, 1.94, 4.27, 9.39, 20.66, 45.46 and 100 mg/L of Clovanyl-3-Formate (CARYOLAN).
At 0.32 mg/L reference substance, the immobilization of daphnia was 40% and 55 % observed at 24 and 48 hours post exposure, respectively.
The EC50 value for Clovanyl-3-Formate (CARYOLAN) at 24 hours was 6.952 mg/L based on nominal concentrations with fiducial limits at 95 % being 5.0398 and 9.5909 mg/L.
The EC50 value for Clovanyl-3-Formate (CARYOLAN) at 48 hours was 3.27 mg/L based on nominal concentrations with fiducial limits at 95 % being 2.2667 and 4.7176 mg/L. - Results with reference substance (positive control):
- At 0.32 mg/L reference substance, the immobilization of daphnia was 40% and 55 % observed at 24 and 48 hours post exposure, respectively.
- Reported statistics and error estimates:
- The EC50 value with 95% fiducial limits, and the equation for the dose-response curve was determined by Probit analysis by the method of Finney (1971) using developed and validated computer program.
Any other information on results incl. tables
TABLE 1. Definitive Test – Results on Daphnia Immobilization
Group |
Treatment (mg/L) |
No. of Daphnia immobilized (5 Daphnia per replicate) |
% Immobilization |
||||||||
24 h |
48 h |
24 h |
48 h |
||||||||
R1 |
R2 |
R3 |
R4 |
R1 |
R2 |
R3 |
R4 |
||||
G1 |
Negative control |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
G2 |
0.4 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
G3 |
0.88 |
1 |
0 |
0 |
0 |
1 |
1 |
1 |
0 |
5 |
15 |
G4 |
1.94 |
1 |
0 |
1 |
2 |
2 |
3 |
2 |
2 |
20 |
45 |
G5 |
4.27 |
1 |
1 |
2 |
2 |
2 |
2 |
4 |
2 |
30 |
50 |
G6 |
9.39 |
3 |
2 |
3 |
2 |
3 |
4 |
3 |
4 |
50 |
70 |
G7 |
20.66 |
4 |
4 |
5 |
4 |
5 |
5 |
5 |
5 |
85 |
100 |
G8 |
45.46 |
4 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
95 |
100 |
G9 |
100 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
100 |
100 |
G10 |
Reference substance 0.32 mg/L K2Cr2O7 |
2 |
3 |
1 |
2 |
3 |
3 |
3 |
2 |
40 |
55 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50 value for Decahydro-1,1,7-trimethyl-3a,7-methano-3aH-cyclopentacyclooct-3-yl formate [Clovanyl-3-Formate (CARYOLAN)] at 48 hours was 3.27 mg/L based on nominal concentrations with fiducial limits at 95 % being 2.2667 and 4.7176 mg/L.
- Executive summary:
The acute immobilization effect of the test item Clovanyl-3-Formate (CARYOLAN) was studied on Daphnia magna. The Daphnia magna of less than 24 hours old were exposed to concentrations of 0.4, 0.88, 1.94, 4.27, 9.39, 20.66, 45.46 and 100 mg/L of Clovanyl-3-Formate (Caryolan) along with a negative and reference substance in reconstituted water at 20 ± 1oC. The number of daphnia immobilized was recorded at 24 and 48 hours post exposure.
There was no immobilization of daphnia in the negative control and at the lowest concentration of 0.4 mg/mL either at 24 or at 48 hours post-exposure. The immobilization of daphniawas 5, 20, 30, 50, 85, 95, and 100 % at 24 hours and 15, 45, 50, 70, 100, 100 and 100 % at 48 hours post-exposure at the tested concentrations of 0.88, 1.94, 4.27, 9.39, 20.66, 45.46 and 100 mg/L of Clovanyl-3-Formate (CARYOLAN).
There was 40 and 55% immobilization of daphnia in thereference substance (potassium dichromate), at 24 and 48-hour post exposure, respectively, indicating the sensitivity of the test procedure used.
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