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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2008.05.20-2008.05.22
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes
Remarks:
The chemical analysis was carried out as accredited analysis but not in compliance with the GLP principles.
Specific details on test material used for the study:
Batch no. 512051
Manufacturing data: December 2005
Origin: Dr. Straetmans Chemische Produkte GmbH, Germany
Form: Almost colourless viscous liquid
Details on test solutions:
The test product is not soluble but dispersible in water.
Test organisms (species):
Daphnia magna
Details on test organisms:
Daphnia magna Straus
The strain has been collected in Langedam, Birkerød, Denmark and cultured at DHI since 1979. The culture is fed with Pseudokirchneriella subcapitata three times a day.
Test type:
semi-static
Water media type:
other: ISO-medium
Total exposure duration:
48 h
Hardness:
252 mg/L expressed as CaCO3
Test temperature:
20.1 ± 0.1°C
pH:
pH 7.7
Nominal and measured concentrations:
Nominal concentrations: 0 (control); 5.0; 10; 20; 50; and 200 mg/L
Details on test conditions:
All test solutions were renewed after 24 hours of exposure.
Reference substance (positive control):
yes
Remarks:
Potassium dichromate, analytical grade (Merck 4864)
Key result
Duration:
24 h
Dose descriptor:
EC10
Effect conc.:
6.01 mg/L
Nominal / measured:
nominal
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
15.1 mg/L
Nominal / measured:
nominal
Basis for effect:
mobility
Results with reference substance (positive control):
A 24-h acute toxicity test on the reference substance was performed at the following concentrations: 0.20; 0.40; 0.70; 1.0; 1.4 and 2.0 mg/L.
The EC50 value for potassium dichromate was 0.99 mg/L based on nominal concentrations.
Reported statistics and error estimates:
The EC10 and EC50 values for the acute toxicity of the test product and reference substance were calculated by use of a standard procedure probit analysis.
Validity criteria fulfilled:
yes
Conclusions:
The results from the 48-hour toxicity test showed that the EC10 (48h) was 6.01 mg/L (3.55 -8.19) and EC50 (48h) was 15.1 mg/L (11.7 -19.5) based on nominal concentrations.
Executive summary:

The toxicity of Dermosoft DGMC to aquatic invertebrates was studied in a test performed according to the OECD guideline no. 202 and following GLP. The test was carried out as a 48-hour semi-static test, i.e. all test solutions were renewed after 24 hours of exposure.

Dermosoft DGMC was tested in the nominal concentrations: 0 (control); 5.0; 10; 20; 50; 100 and 200 mg/L.

The chemical analyses of NVOC showed that the average recovery for the total exposure period was very high in all subsamples collected. The mean recovery was ≥80% in all test solutions from 5 mg/mL to 200 mg/L during the test. The statistical calculations were thus made on basis of the nominal concentrations.

The data analysis showed that the EC10 (48h) was 6.01 mg/L (3.55 -8.19) and EC50 (48h) was 15.1 mg/L (11.7 -19.5) based on nominal concentrations.

Description of key information

The results from the 48-hour toxicity test showed that the EC10 (48h) was 6.01 mg/L (3.55 -8.19) and EC50 (48h) was 15.1 mg/L (11.7 -19.5) based on nominal concentrations.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
15.1 mg/L

Additional information