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EC number: 228-536-2 | CAS number: 6290-17-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 13 March 2002 to 15 March 2002
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- The test item was analytically verified via DOC analysis. However. no further detail on analysis is provided in the study report.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 1984
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- 1992
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Niedersächsisches Umweltministerium, Hannover, Germany (d.d. 2002-03-11)
- Specific details on test material used for the study:
- - Solubility in water: 71.70 g/L (at room temperature)
- pH value: 6.4 (20 °C)
- Storage at the test facility: Refrigerator 7 ± 2 °C, protected from light and moisture
- ThTOC: 56.64%
- Expiry date: 2002-08-31 - Analytical monitoring:
- yes
- Details on sampling:
- After 0 h (new media) and 48 h (old media).
- Vehicle:
- no
- Details on test solutions:
- - Stock solution: 100 mg/L, prepared with dilution water
- Dispersion treatment: Agitation
- Evidence of undissolved material: No, the test item was clearly dissolved throughout exposure. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Strain/clone: STRAUS (Clone 5)
- Source: Institut für Wasser-, Boden- und Lufthygiene des Bundesgesundheitsamtes, Corrensplatz, 14195 Berlin, Germany.
- Breeder: Dr.U.Noack-Laboratorium, 31157 Sarstedt, Germany.
- Cullture conditions: In 2-3 L glass vessels with approximatively 1.8 L culture medium (Elendt M4 modified to a total hardness of 160-180 mg CaCO3/L), at 21 °C (temperatures of 20-25 °C were tolerated), in an incubator, 16 h illumination, illumination strength max 20 µmol·m-2·s-1.
- Age of test organisms: 2 - 24 h old (obtained by removing the mother animals twice within 22 h)
- Food type/amount/frequency: 5x weekly ad libitum with a mix of Scenedesmus subspicatus and Chlorella vulgaris (algae cell density of >1.0E+06 cells/mL).
- Feeding during test: No
ACCLIMATION
- Acclimation period: 2 h
- Acclimation conditions: same as test - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 248 mg/L CaCO3 (dilution water)
- Test temperature:
- 20 ± 1 °C.
- pH:
- - Start: 7.95 (limit concentration), 7.79 (control)
- End: 7.74 - 7.64 (limit concentration), 7.54 - 7.59 (control) - Dissolved oxygen:
- - Start: 8.25 mg/L (limit concentration), 8.20 mg/L (control)
- End: 7.80 - 7.85 mg/L (limit concentration), 7.80 - 7.87 mg/L (control) - Conductivity:
- 659 µS/cm (dilution water)
- Nominal and measured concentrations:
- - Nominal test concentrations: 0 (control) and 100 mg/L.
- Measured test concentrations: Recovery was see 'Any other information on materials and methods incl. tables'. - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers (5 cm ID x 8 cm H) with a nominal volume of 50 mL
- Fill volume: 20 mL
- Type (open / closed): not specified
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: According to EEC 92/69 L383A C.2.
- Physico-chemical parameters: Prior to the start of the test, the pH-value, dissolved oxygen concentration, temperature, conductivity and total hardness of the test medium were measured. At the start of the test, the pH-value and dissolved oxygen concentration were measured in one additional replicate per concentration and control. After 48 h the same water parameters were measured in all replicates per concentration and control. The temperature was recorded throughout the test with a thermohygrograph.
OTHER TEST CONDITIONS
- Illumination: Diffuse light, 16 h illumination strength max. 20 µmol·m-2·s-1 (corresponding to 1200 lux).
- Photoperiod: 16/8 h light/dark cycle
- Equipment used: Oxygen: Oximeter, Oxi 530 (WTW); pH value: pH-Meter inoLab pH Level 1 (WTW); Temperature: Hygro-thermograph (Lufft); Water hardness: Spectrophotometer Cadas 100 LPG 158 (Dr. Lange); DOC-analyzer: multi N/C 3000, Analytik Jena
EFFECT PARAMETERS MEASURED
Immobilisation of daphnia after 24 and 48 hours exposure.
RANGE-FINDING STUDY
- Test concentrations: 1, 10 and 100 mg/L (non-GLP)
- Results used to determine the conditions for the definitive study: Yes. No biologically significant effect (≤10%) was seen at the highest test concentration (nominal 100 mg/L) after 48 h of exposure. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate (Feb/March 2002)
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- There was no biologically significant effect (≤10%) neither in the tested limit concentration nor in the control group.
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Relevant effect levels: 24-h EC50 = 1.4 mg/L (C.L. 1.1 - 1.8 mg/L). - Reported statistics and error estimates:
- EC50 values were only calculated for the reference item via probit analysis. Calculation of the confidence intervals was carried out using standard procedures according to BREMG & TÜMPLING (1982)
- Validity criteria fulfilled:
- yes
- Remarks:
- See 'Any other information on materials and methods incl. tables'.
- Conclusions:
- The 48-h EC50 is >100 mg/L in freshwater aquatic invertebrates (Daphnia magna) based on nominal concentration.
- Executive summary:
The acute toxicity of the substance to aquatic invertebrates was determined in a limit test according to OECD TG 202 and in compliance with GLP criteria. Daphnids (Daphnia magna, 4 replicates of 5 ) were exposed to 100 mg/L test item and a blank control for 48 hours under static conditions. The test item was measured via DOC analysis at the start of the test and after 48 hours of exposure, and recovery was determined to be 96.8 % (based on ThTOC).
After 24 and 48 hours of exposure, no biologically significant effect (≤10% immobilization) was observed in the tested limit concentration nor in the control group. Based on these findings the 48-h EC50 value was determined to be >100 mg/L (based on nominal concentration). All the OECD validity criteria were met.
Reference
Description of key information
The acute toxicity of the substance to aquatic invertebrates was determined in a limit test according to OECD TG 202 and in compliance with GLP criteria (Dr Noack, 2002). Daphnids (Daphnia magna, 4 replicates of 5 ) were exposed to 100 mg/L test item and a blank control for 48 hours under static conditions. The test item was measured via DOC analysis at the start of the test and after 48 hours of exposure, and recovery was determined to be 96.8 % (based on ThTOC).
After 24 and 48 hours of exposure, no biologically significant effect (≤10% immobilization) was observed in the tested limit concentration nor in the control group. Based on these findings the 48-h EC50 value was determined to be >100 mg/L (based on nominal concentration). All the OECD validity criteria were met.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 100 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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