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EC number: 214-686-6 | CAS number: 1185-57-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Remarks:
- Experimental data of read across chemicals
- Justification for type of information:
- Data from handbook or collection of data
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- other: Refer below principle
- Principles of method if other than guideline:
- WoE was prepared to determine the effect of test chemical on the mobility of aquatic invertebrates.
- GLP compliance:
- no
- Analytical monitoring:
- not specified
- Vehicle:
- not specified
- Details on test solutions:
- WoE 2: The stock solution 200.0 mg/l was prepared by dissolving white powder in reconstituted water. The test solutions of required concentrations were prepared by mixing the stock solution of the test sample in reconstituted water.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- WoE 2: TEST ORGANISM
- Common name: Water flea
- Strain: Straus
- Source: Own breeding at University of Chemistry and Technology, Prague
- Age at study initiation (mean and range, SD): The animals used for the test shall be less than 24 h old and should not be first brood progeny
- Feeding during test: No feeding
ACCLIMATION - No data available
- Acclimation period:
- Acclimation conditions (same as test or not):
- Type and amount of food:
- Feeding frequency:
- Health during acclimation (any mortality observed): - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- ± 1 hr
- Test temperature:
- WoE 2: 20±1°C
- pH:
- WoE 2: sample at concentration 200.0 mg/l: pH = 6.2 changed to pH = 7.0 during the test,
control: pH = 8.0 did not change during the test - Dissolved oxygen:
- WoE 2: higher than 8.2 mg/L at the end of test
- Nominal and measured concentrations:
- WoE 2: 0,18,30,49,78,125,200 mg/l nominal concentrations were used
- Details on test conditions:
- WoE 2: TEST SYSTEM
- Test vessel: 50 ml glass vessel
- fill volume: 25 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
Natural water (surface or ground water), reconstituted water or dechlorinated tap water are acceptable as culturing and dilution water if D. magna survives in it for the duration of the culturing, acclimation and testing without showing signs of stress. Waters in the range pH 6 to pH 9, with hardness between 140 mg/l and 275 mg/l (as CaCO3) are recommended.
As an example, the preparation of dilution water meeting the requirements is described below.
Dissolve known quantities of reagents in water. The dilution water prepared shall have a pH of 7.8 ± 0.5, a hardness of (225 ± 50) mg/l (expressed as CaCO3), a molar Ca + Mg ratio close to 4 + 1 and a dissolved oxygen concentration above 7 mg/l.
Prepare the solutions specified below:
- Calcium chloride solution: Dissolve 117.6 g of calcium chloride dihydrate (CaCl2.2H2O) in water (4.2) and make up to 1 l with water (4.2).
- Magnesium sulfate solution: Dissolve 49.3 g of magnesium sulfate heptahydrate (MgSO4.7H2O) in water (4.2) and make up to 1 l with water (4.2).
- Sodium bicarbonate solution: Dissolve 25.9 g of sodium bicarbonate (NaHCO3) in water (4.2) and make up to 1 l with water (4.2).
- Potassium chloride solution: Dissolve 2.3 g of potassium chloride (KCI) in water (4.2) and make up to 1 l with water (4.2).
Mixing
Mix 2.5 ml of each of the four solutions and make up to 1 l with water.
The dilution water shall be aerated until the dissolved oxygen concentration has reached saturation and the pH has stabilized. If necessary, adjust the pH to 7.8 ± 0.5 by adding sodium hydroxide (NaOH) solution or hydrochloric acid (HCI). The dilution water prepared in this way shall not be further aerated before use.
- Sodium hydroxide solution, e.g. [NaOH] : 1 mol/l.
- Hydrochloric acid, e.g. [HCl] : 1 mol/l.
Reference substance:
Dissolve 600 mg of potassium dichromate (K2Cr2O7) in water and make up to 1 l with water (4.2).
OTHER TEST CONDITIONS
- Adjustment of pH: no adjustment done
- Photoperiod: No - Darkness
- Light intensity:
CALCULATION:
EC50 was calculated using non linear regression by the software Prism 4.0 - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate (K2Cr2O7)
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 116.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95 % CI: 88.5-152.5 mg/l
- Remarks:
- WoE 2
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: WoE 3
- Results with reference substance (positive control):
- WoE 2: - Results with reference substance valid
- EC50: 0.73 mg/L (24 hours) - Reported statistics and error estimates:
- WoE 2: EC50 was calculated using non linear regression by the software Prism 4.0
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the overall result observations and effects on the mobility of aquatic invertebrate Daphnia magna, it was observed that the EC50 was ranges from > 100 mg/l to 116.2 mg/l.
- Executive summary:
Data available for the test chemical have been reviewed to determine the toxicity of test chemical on aquatic invertebrates Daphnia magna. The studies are as mentioned below:
Aim of this study was to assess the short term toxicity of test chemical to aquatic invertebrates Daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs. 0,18, 30, 49, 78,125, 200 mg/l nominal concentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. The median effective concentration (EC50) of the test substance on Daphnia magna was determined to be 116.2 mg/L on the basis of mobility inhibition effects in a 48 hour study. Based on the EC50 value, substance consider likely to be non-hazardous to aquatic invertebrate and cannot be classified as per the CLP classification criteria.
Above study was further supported by the study from authoritative database. Principle of this study was to determine the effect of test chemical on the immobility of Daphnia magna in accordance with OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test). Test conducted under the static system for 48 hours. Based on the immobility of Daphnia magna by the test chemical exposure for 48 hours, the EC50 was observed to be > 100 mg/l. Thus based on the EC50 value, test chemical consider to be nontoxic and not classified as per the CLP classification criteria.
Thus based on the above both studies, it is observed that the test chemical was nontoxic and not classified as per the CLP classification criteria.
Reference
Description of key information
Based on the overall result observations and effects on the mobility of aquatic invertebrate Daphnia magna, it was observed that the EC50 was ranges from > 100 mg/l to 116.2 mg/l.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 116.2 mg/L
Additional information
Data available for the test chemical, structurally and functionally similar read across chemicals have been reviewed to determine the toxicity of test chemical on aquatic invertebrates Daphnia magna. The studies are as mentioned below:
Aim of this study was to assess the short term toxicity of test chemical to aquatic invertebrates Daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs. 0,18, 30, 49, 78,125, 200 mg/l nominal concentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. The median effective concentration (EC50) of the test substance on Daphnia magna was determined to be 116.2 mg/L on the basis of mobility inhibition effects in a 48 hour study. Based on the EC50 value, substance consider likely to be non-hazardous to aquatic invertebrate and cannot be classified as per the CLP classification criteria.
Above study was further supported by the study from authoritative database. Principle of this study was to determine the effect of test chemical on the immobility of Daphnia magna in accordance with OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test). Test conducted under the static system for 48 hours. Based on the immobility of Daphnia magna by the test chemical exposure for 48 hours, the EC50 was observed to be > 100 mg/l. Thus based on the EC50 value, test chemical consider to be nontoxic and not classified as per the CLP classification criteria.
Thus based on the above both studies, it is observed that the test chemical was nontoxic and not classified as per the CLP classification criteria.
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