Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 947-036-1 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 17 April - 24 July 2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- not required
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
A stock solution with a nominal concentration of 3200 mg/L was prepared with direct addition of the powdered test item, mixed into deionised water using ultrasonic bath for approximately 6 minutes. The test formulation was freshly prepared at the beginning of the experiment, in the testing laboratory. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Species: Activated sludge, microorganisms from a domestic waste water treatment plant.
Source: The activated sludge was supplied from the sewage plant for domestic sewage in Veszprém, Hungary
Conditioning: The activated sludge was supplied by the sewage plant for domestic sewage two days before the start of the experiment. During holding prior to use the sludge was fed daily with 50 mL synthetic sewage per litre and kept aerated at 20 ± 2°C until use.
The activated sludge used for this study was washed and centrifuged and the supernatant liquid phase was decanted. The solid material was re-suspended in chlorine-free tap water and again centrifuged.
An aliquot of the final sludge suspension was weighed, dried and the ratio of wet sludge to dry weight determined. Based on this ratio, calculated amounts of wet sludge were suspended in chlorine-free tap water to yield a concentration equivalent to 3 g per litre (on dry weight basis). The pH of the activated sludge inoculum was determined to be pH 7.75. The activated sludge was used directly after conditioning. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Hardness:
- No data
- Test temperature:
- 21.0 – 21.9 °C
- pH:
- 7.56 - 8.19
- Salinity:
- No data
- Conductivity:
- No data
- Nominal and measured concentrations:
- Preliminary test: 10, 100, 1000 mg/L (nominal)
Definitive test: 59.60, 95.37, 152.59, 244.14, 390.63, 625.0, 1000.0 mg/L (nominal) - Details on test conditions:
- TEST SYSTEM
- Test vessel: Appropriate glass beakers for 500 mL volume and BOD bottles with 300 mL volume.
- Aeration: With compressed air (about 1 L/min)
- No. of vessels per concentration (replicates): 5
- No. of vessels per reference control (replicates): 1
- No. of vessels per untreated control (replicates): 8
- No. of vessels per abiotic control (replicates): Not required as no oxygen uptake in the preliminary test.
- Sludge concentration (weight of dry solids per volume): 3g sludge innoculum/L of suspended solids
- Nutrients provided for bacteria: Synthetic Sewage Feed : Peptone 16.0 g; Meat extract 11.0 g; Urea 3.0 g; NaCl 0.7 g; CaCl2 x 2H2O 0.4 g; MgSO4 x 7H2O 0.2 g; K2HPO4 2.8 g; Deionised water add. 1000.0 mL
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Deionised water
OTHER TEST CONDITIONS
- Adjustment of pH: no
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Inhibition of respiration rate.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.6
- Range finding study
- Test concentrations: 10, 100, 1000 mg/L (nominal)
- Results used to determine the conditions for the definitive study: 10 mg/L: 3.49 % inhibition; 100 mg/L: 36.73 % inhibition; 1000 mg/L: 67.51 % inhibition - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol at nominal test concentrations of 1.0; 3.2; 10 and 32 mg/L
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 99.54 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- - The average specific total respiration rate of the untreated (blank) controls was 30.07 mg O2/L/h/g solid, so the validity criteria was met.
- The variation coefficient of oxygen uptake rate in above untreated replicates was 17.94 % and thus, the validity criteria was met. - Results with reference substance (positive control):
- - Results with reference substance valid? Yes. The EC50 values of the positive control substance 3,5-Dichlorophenol (3,5-DCP) were in the recommended range for total respiration using activated sludge derived from domestic sewage.
- Relevant effect levels: The 3-hour EC50 of 3,5-Dichlorophenol was calculated to be 12.88 mg/L with 95% confidence limits of 10.51 – 15.79 mg/L. - Reported statistics and error estimates:
- The 3-hour EC20, EC50 and EC80 values of the test item and the 3-hour EC50 values of the reference item with their 95 %-confidence limits were calculated by Probit analysis using TOXSTAT software.
- Validity criteria fulfilled:
- yes
- Conclusions:
- With respect to the inhibitory effect of the test item on the total respiration rate, the 3-hour EC50 value of the test item was determined to be in the range of 10 mg/L to 100 mg/L (99.54 mg/L was calculated, with 95 % confidence limits of 65.74 – 150.71 mg/L).
- Executive summary:
The effect of the substance on the respiration rate of activated sludge microorganisms was investigated by the method as described in the OECD Guidelines for Testing of Chemicals, No.: 209 Adopted: 22 July 2010.
Since significant inhibition effect (on the total respiration rate of the microorganisms of the activated sludge) was detected in the preliminary test, a further definitive test was conducted with the following concentrations: 59.60, 95.37, 152.59, 244.14, 390.63, 625.0 and 1000.0 mg/L.
The abiotic control did not need to be tested as there was no oxygen uptake in the preliminary test.
The purpose of the test was only to assess the effects on total oxygen uptake, the inhibition effect in respect of nitrification process was not determined.
The reference control results showed that in this study, the inoculum and methodology used provided a good measure of inhibition of activated sludge respiration rate.
With respect to the inhibitory effect of the test item on the total respiration rate, the 3-hour EC50 value of Amphosol CDB special Dried was determined to be in the range of 10 to 100 mg/L (99.54 mg/L was calculated, with 95 % confidence limits of 65.74 – 150.71 mg/L).
Reference
Description of key information
3 -h EC50 = 99.54 mg/L (OECD TG 209).
Key value for chemical safety assessment
- EC50 for microorganisms:
- 99.54 mg/L
Additional information
The effect of the substance on the respiration rate of activated sludge microorganisms was investigated by the method as described in OECD TG 209 Adopted: 22 July 2010.
Since significant inhibition effect (on the total respiration rate of the microorganisms of the activated sludge) was detected in the preliminary test, a further definitive test was conducted with the following concentrations: 59.60, 95.37, 152.59, 244.14, 390.63, 625.0 and 1000.0 mg/L.
The abiotic control did not need to be tested as there was no oxygen uptake in the preliminary test.
The purpose of the test was only to assess the effects on total oxygen uptake, the inhibition effect in respect of nitrification process was not determined.
The reference control results showed that in this study, the inoculum and methodology used provided a good measure of inhibition of activated sludge respiration rate.
With respect to the inhibitory effect of the test item on the total respiration rate, the 3-hour EC50 value of Amphosol CDB special Dried was determined to be in the range of 10 to 100 mg/L (99.54 mg/L was calculated, with 95 % confidence limits of 65.74 – 150.71 mg/L).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.