Ethyl undec-10-enoate

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

29 Jul - 01 Sep 1993

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1993

Report date:

1993

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

Deviations:

yes

Remarks:

activated sludge was used instead of effluent

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Isopropyl laurate

EC Number:

233-560-1

EC Name:

Isopropyl laurate

Cas Number:

10233-13-3

Molecular formula:

C15H30O2

IUPAC Name:

isopropyl laurate

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Secondary activated sludge obtained from the Waste water treatment plant Nieuwgraaf in Duiven, The Netherlands on 29th July 1993; treating predominantly domestic waste water
- Pretreatment: Sludge was preconditioned to reduce the endogenous respiration rates; aerated for one week
- Concentration of sludge: 200 mg DW/L
- Test concentration of sludge: 2 mg DW/L

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

7.1 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Composition of medium: nutrient medium containing 8.5 mg/L KH2PO4, 21.75 mg/L K2HPO4, 33.3 mg/L Na2HPO4, 22.5 mg/L MgSO4, 27.5 mg/L CaCl2 and 0.25 mg/L FeCl3
- Test temperature: 21 ± 1 °C
- pH: 7.1 at begin, 6.8 and 6.6 at test end
- Continuous darkness: yes

TEST SYSTEM
- Culturing apparatus: BOD bottles, 250 or 300 mL volume
- Number of culture flasks/concentration: 10 bottles containing only inoculum, 10 bottles containing inoculum + test substance, 10 bottles containing sodium acetate and inoculum
- Measuring equipment: oxygen electrode (WTW Trioxmatic EO 200) and oxygen meter (WTW OXI 530);

SAMPLING
- Sampling frequency: Days 0, 7, 14, 21 and 28
- Sampling method: Removal of two bottles per measuring date

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes, 10 replicates

Reference substance

Reference substance:

acetic acid, sodium salt

Results and discussion

% Degradation

Parameter:

% degradation (O2 consumption)

Value:

67

Sampling time:

28 d

Details on results:

The substance is readily biodegradable (pass level > 60% within 14 days)

BOD5 / COD results

Results with reference substance:

The reference substance (sodium acetate; 6.7 mg/L) was biodegraded to 77% after 28 days.

Any other information on results incl. tables

Table 1: Biodegradation in %:

	Biological degradation
Test substance	% BOD 0 days	% BOD 7 days	% BOD 14 days	% BOD 21 days	% BOD 28 days
CAS 10233-13-3	0	48	62	68	67
Na-acetate-3-hydrate	0	69	77	-	-

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable