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Diss Factsheets
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EC number: 211-743-7 | CAS number: 693-13-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / bone marrow chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- The detailed protocols are described by Witt et al. (1999). Bone marrow tests were initially performed using the standard three-exposure protocol described in detail by Shelby et al. (1993).
- GLP compliance:
- not specified
- Type of assay:
- other: bone marrow micronucleus test
Test material
- Reference substance name:
- N,N'-methanetetraylbis(1-methylethylamine)
- EC Number:
- 211-743-7
- EC Name:
- N,N'-methanetetraylbis(1-methylethylamine)
- Cas Number:
- 693-13-0
- Molecular formula:
- C7H14N2
- IUPAC Name:
- (propan-2-yl)({[(propan-2-yl)imino]methylidene})amine
- Test material form:
- liquid
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- B6C3F1
- Sex:
- male
Administration / exposure
- Route of administration:
- intraperitoneal
- Vehicle:
- corn oil
- Details on exposure:
- Single injection bone marrow micronucleus tests were conducted with diisopropylcarbodiimide in B6C3F1 male mice. These studies were designed to permit the testing of higher doses than could be administered in the multiple treatment protocol described above. The positive control was again cyclophosphamide. Two different posttreatment samples were collected; peripheral blood smears were prepared 48 hours after injection and bone marrow slides were prepared 24 and 48 hours after injection.
- Duration of treatment / exposure:
- single injection
Examinations
- Tissues and cell types examined:
- Air-dried smears from all the bone marrow micronucleus tests were fixed in absolute methanol and stained with acridine orange (Tice et al., 1990); 2,000 PCEs were scored per animal for frequency of micronucleated cells in blood, bone marrow, or both from up to five animals per group. In addition, the percentage of PCEs among the total erythrocyte population in the bone marrow was scored for each dose group as a measure of toxicity.
Results and discussion
Test results
- Key result
- Sex:
- male
- Genotoxicity:
- negative
- Toxicity:
- not specified
- Vehicle controls validity:
- not specified
- Negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Additional information on results:
- Diisopropylcarbodiimide was then tested for induction of micronucleated PCEs in male B6C3F1 mice, the same strain employed in the 3-month dermal study, and the results of this test, assaying the frequency of micronucleated PCEs in bone marrow, were negative (Table E3).
Applicant's summary and conclusion
- Conclusions:
- Diisopropylcarbodiimide was then tested for induction of micronucleated PCEs in male B6C3F1 mice, the same strain employed in the 3-month dermal study, and the results of this test, assaying the frequency of micronucleated PCEs in bone marrow, were negative (Table E3).
- Executive summary:
Diisopropylcarbodiimide was then tested for induction of micronucleated PCEs in male B6C3F1 mice, the same strain employed in the 3-month dermal study, and the results of this test, assaying the frequency of micronucleated PCEs in bone marrow, were negative (Table E3). The values of PCE percentages in this mouse study were unchanged with increasing dose, even though chemical-related toxicity was noted at the two highest doses tested. To permit the administration of a higher dose of diisopropylcarbodiimide in mice and to allow scoring of both blood and bone marrow erythrocytes in the same animals, single injection micronucleus studies were conducted with sampling at 24 and 48 hours posttreatment.
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