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Diss Factsheets
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EC number: 946-329-1 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 1974
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods with acceptable restrictions
Data source
Reference
- Reference Type:
- publication
- Title:
- Mutagenicity of chromates in bacteria and its relevance to chromate carcinogenesis
- Author:
- Venitt S, Levy LS
- Year:
- 1 974
- Bibliographic source:
- Nature 250, 493 - 495 (09 August 1974)
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- E.coli WP2 strains were grown overnight in nutrient broth at 37° C and diluted 1:50 in enriched M9 medium. Cells were collected during mid-logarithmic phase. 5 x 10E7 bacteria were spread on agar plates containing M9 medium, 0.4% (w/v) casamino-acids and 1 µg/mL L-trypophan. Test compounds were dissolved in deionised water, filter-sterilised, and 5 µL applied to the centre of each plate. Pre-existing revertants were assayed on agar plates containing M9 medium. Plates were incubated at 37° C and the maximum yield of mutants (that is, revertants to tryptophan prototrophy) was obtained 4-5 d after plating.
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Zinc
- EC Number:
- 231-175-3
- EC Name:
- Zinc
- Cas Number:
- 7440-66-6
- IUPAC Name:
- zinc
- Test material form:
- not specified
Constituent 1
- Specific details on test material used for the study:
- not specified
Method
- Target gene:
- trpE locus
Species / strainopen allclose all
- Species / strain / cell type:
- E. coli WP2
- Species / strain / cell type:
- E. coli WP2 uvr A
- Species / strain / cell type:
- E. coli WP2
- Remarks:
- exrA
- Metabolic activation:
- without
- Test concentrations with justification for top dose:
- not specified
- Vehicle / solvent:
- deionised water, filter-sterilised
Controls
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- not specified
- Positive control substance:
- not specified
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
- Cell density at seeding (if applicable): 5x10E7
DURATION
- Preincubation period: overnight
- Exposure duration: 4-5 days - Statistics:
- t-test
Results and discussion
Test resultsopen allclose all
- Species / strain:
- E. coli WP2
- Metabolic activation:
- without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- not examined
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- not examined
- Species / strain:
- E. coli WP2
- Remarks:
- E. coli WP2 exrA
- Metabolic activation:
- without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- not examined
Applicant's summary and conclusion
- Conclusions:
- Zinc salts were negative in three different E. coli strains.
- Executive summary:
The mutagenicity of zinc salts on three different E. coli strains was tested in a bacterial reverse mutation assay on agar plates. Zinc salts were negative in all three strains tested.
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