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Diss Factsheets
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EC number: 906-936-4 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to other aquatic organisms
Administrative data
- Endpoint:
- toxicity to other aquatic vertebrates
- Type of information:
- read-across based on grouping of substances (category approach)
- Adequacy of study:
- supporting study
- Study period:
- Oct 28, 1994 - Dec 19, 1994
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Non-GLP study following ASTM (1988) protocol
- Justification for type of information:
- Read across is based on the category approach. Please refer to attached category document.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: ASTM (American Society for Testing and Materials). 1988. Guide for Conducting Static Acute Toxicity Tests with Fishes, Macroinvertebrates, and Amphibians. Annual Book of ASTM Standards. Volume 1..04.E 729 -88el.
- GLP compliance:
- no
Test material
- Reference substance name:
- 2,2'-oxydiethanol
- EC Number:
- 203-872-2
- EC Name:
- 2,2'-oxydiethanol
- Cas Number:
- 111-46-6
- Molecular formula:
- C4H10O3
- IUPAC Name:
- 2,2'-oxydiethanol
- Test material form:
- other: clear liquid
- Details on test material:
- Diethylene glycol was purchased from Sigma Chemical Company (St. Louis, MO). The test substance was contained in opaque polypropylene containers (4 L size) or in amber glass bottles (1 L size). Prior to use in toxicity tests, the test substance was stored in darkness at room temperature. The test substance was analytical grade and was tested 100% active ingredient.
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not applicable.
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Chemical analyses were conducted at MDS Environmental Services (Environmental Laboratory, Mississauga, Ontario) with samples collected during the toxicity testing phase (at the beginning and end of each exposure period for each concentration, or when there was complete mortality of test organisms). Submission of samples for chemical analysis were selected based on test organism response (to bracket the concentration with no mortality, partial mortality, and/or complete mortality). Samples from control solutions were submitted for chemical analysis from the end of the test and for "background check" only. Chemical analyses were conducted for each replicate for each concentration bracketing the LC50 concentration. Glycol analyses were conducted using GC/FID direct aqueous injection.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Dilution water quality was continuously monitored and documented. Laboratory dilution water was analyzed for metals, organic and inorganic chemicals according to various daily, weekly, monthly, quarterly and annual schedules, as appropriate for each parameter.
All aqueous testing is conducted using the well-established practice of negative (clean) controls consisting of organisms handled and treated in an identical manner to sample exposed organisms but exposed only to dilution water (i.e., no test substance).
Test organisms
- Test organisms (species):
- Xenopus laevis
- Details on test organisms:
- Adult Zenopus laevis (African clawed frog) were obtained from Carolina Biological Supply. The adults were injected with chlorionic gonadotropin hormone, and were mated to produce fertilized eggs in-house. Juvenile frogs were raised from eggs until the appropriate stage was reached.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- No additional information.
Test conditions
- Hardness:
- 130-140 mg/l
- Test temperature:
- 20-21 degrees Celsius
- pH:
- 7.74-8.2
- Dissolved oxygen:
- 7.6-8.6
- Salinity:
- Alkalinity as CaCO3: > 20 mg/l
- Nominal and measured concentrations:
- 0, 6250, 12500, 25000, 50000, 100000 (nominal)
The concentrations of DEG in exposure vessels were measured at the end of tests and were within 14% of initial concentrations. - Details on test conditions:
- 5 organisms/test vessel
Duplicate test vessels
Test vessels were plastic, 200 ml - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 20 358 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: 17285-23978
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 20 496 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: 15679-26484
- Details on results:
- In replicate A, the 48 hr LC50 was 20,358 mg/l with a 95% confidence interval of 17,285-23,978 mg/l.
In replicate B, the 48 hr LC50 was 20,496 mg/l with a 95% confidence interval of 15,679-26,484 mg/l. - Results with reference substance (positive control):
- No data
- Reported statistics and error estimates:
- LC50 was calculated by the Spearman-Karber Method for the first replicate, and Probit Method for the 2nd replicate.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In replicate A, the 48 hr LC50 was 20,358 mg/l with a 95% confidence interval of 17,285-23,978 mg/l.
In replicate B, the 48 hr LC50 was 20,496 mg/l with a 95% confidence interval of 15,679-26,484 mg/l. - Executive summary:
In replicate A, the 48 hr LC50 was 20,358 mg/l with a 95% confidence interval of 17,285-23,978 mg/l.
In replicate B, the 48 hr LC50 was 20,496 mg/l with a 95% confidence interval of 15,679-26,484 mg/l.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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