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EC number: 206-033-9 | CAS number: 294-62-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1994-10-25 to 1994-10-28
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with acceptable restrictions: Test substance not stable under test condition
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Version / remarks:
- Cited as Directive 92/69/EEC, C.3
- GLP compliance:
- yes
Test material
- Reference substance name:
- Cyclododecane
- EC Number:
- 206-033-9
- EC Name:
- Cyclododecane
- Cas Number:
- 294-62-2
- Molecular formula:
- C12H24
- IUPAC Name:
- cyclododecane
- Details on test material:
- Test substance: cyclododecane of Hüls AG, produced 19 May 1993, purity 99.8% (GC-FID area); ID No. 0637/81524, PR No. 1452/931213
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
- Details on test solutions:
- STOCK AND TEST SOLUTION AND THEIR PREPARATION
- 1 g/l stirred in deionized water for about 18 h and filtered (Water Accomodated Fraction)
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISMS
- Strain: Scenedesmus subspicatus CHODAT (86.81 SAG)
- Source/supplier: Origin: Institut fuer Wasser-, Boden- und Lufthygiene, Berlin, further bred inhouse
- Laboratory culture: From a stock culture, a preculture is seeded three days before begin of test. Test cultures are seeded from the latter
- Method of cultivation: Erlenmeyer flasks under light benches
- Controls: yes
- Initial cell concentration: ca. 20 000 cells/ml
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 22.6-23.2 degree C
- pH:
- 8.3-8.9 (begin) 8.3-8.7 (end of study)
- Nominal and measured concentrations:
- - Concentrations: 0.14; 0.23; 0.39; 0.69; 1.2; 2.1 mg/l (nominal)
- Details on test conditions:
- GROWTH/TEST MEDIUM CHEMISTRY
- see Test Guideline
TEST SYSTEM
- Renewal of test solution: no
- Exposure vessel type: Erlenmeyer flasks under light benches
- Number of replicates: 5 or 8, probably 8 for control, 5 for each concentration > 0
- Intensity of irradiation: approximately 8,000 lux, white
TEST PARAMETER
- photometric determination of cell numbers at 685 nm, calibration with standard curve - Reference substance (positive control):
- not specified
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 2.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 2.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
Any other information on results incl. tables
- Cell density data: (x 1.0E+04 cells/ml)
concentration 0 hours 24 hours 48 hours 72 hours
control 2 3 18 73
0.14 mg/l 2 4 17 82
0.23 mg/l 2 4 16 80
0.39 mg/l 2 4 20 88
0.69 mg/l 2 5 19 78
1.20 mg/l 2 4 18 76
2.10 mg/l 2 4 17 85
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The EC50 (72 h, based on growth rate) was determined as >2.1 mg/L indicating that no toxic effects were observed on algae up to the highest soluble concentration.
- Executive summary:
The growth inhibition of cyclododecane on Scenedesmus subspicatus was studied in a 72 -h growth inhibion test by Hüls AG (1995) according to EU method C.3. Algae cell numbers were determined at six test concentrations ranging from 0.14 mg/L to 2.1 mg/L plus controls at dailiy intervals. No effects were observed at any test concentration within the study period. Thus the EC50 was determined to be >2.1 mg/L. The study was assessed as "reliable with restrictions".
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