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EC number: 293-165-5 | CAS number: 91052-08-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 18 Jul- 22 Jul 1988
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with acceptable restrictions (no analytics performed)
- Qualifier:
- according to guideline
- Guideline:
- ISO 7346-1 (Determination of the Acute Lethal Toxicity of Substances to a Freshwater Fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)] - Part 2: Semi-static method)
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Test substance was directly weighed into the test vessels, filled up to final volume of 5 L with standard dilution water and afterwards stirred 10 seconds by Ultraturrax
- Controls: yes - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebra fish
- Feeding during test: none
- Pre-treatment: none
ACCLIMATION
- Health during acclimation: Mortality prior to test < 0.1% - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- About 250 mg CaCO3/L
- Test temperature:
- 22.5 - 23.5°C
- pH:
- 7.5 - 8.0
- Dissolved oxygen:
- 60-98% air saturation values
- Nominal and measured concentrations:
- Nominal concentrations: 1000, 3000 and 10000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 10L fish basins, containing 5L test water
- Renewal rate of test solution: daily
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: standard dilution water
OTHER TEST CONDITIONS
- Photoperiod: 16 hours illumination per day
EFFECT PARAMETERS MEASURED: Mortality, recorded at 0, 6, 24, 48, 72 and 96 hours
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3 - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- >= 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: The fish did not show any abnormal behaviour.
- Mortality of control: no - Sublethal observations / clinical signs:
No effect up to the limit of the water solubility of the test substance was observed.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 08 - 12 Jan 1990
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP Guideline study with acceptable restrictions: No analytics of the test substance was performed.
- Qualifier:
- according to guideline
- Guideline:
- ISO 7346-1 (Determination of the Acute Lethal Toxicity of Substances to a Freshwater Fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)] - Part 2: Semi-static method)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test substance was directly added to the test vessels. The vessels were filled up to final volumes of 5L with standard solution water and then were stirred 3 minutes by ultraturrax. - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: zebra fish
- Feeding during test: none
ACCLIMATION
- Health during acclimation (any mortality observed): Mortality prior to test was < 0.1%. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 250 mg/L as CaCO3
- Test temperature:
- 22.0 - 23.5%
- pH:
- 7.6 - 7.8
- Dissolved oxygen:
- 45 - 97% saturation
- Nominal and measured concentrations:
- Nominal: 100, 300, 1000, 3000 and 10000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: fish basins
- Size, fill volume: 10 L, 5 L
- Renewal rate of test solution: daily
- No. of organisms per vessel: 10
- No. of vessels per concentration: 1
- No. of vessels per control: 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: standard dilution water
OTHER TEST CONDITIONS
- Photoperiod: 16h light per day
EFFECT PARAMETERS MEASURED: Mortality was recorded after 0, 6, 24, 48, 72 and 96h test duration. - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: No abnormal behaviour was observed.
- Mortality of control: No mortality was observed in the control and in any treatment. - Sublethal observations / clinical signs:
- 0% mortality reported in both treatment and control vessels
- Endpoint:
- short-term toxicity to fish
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- 18 Jul- 22 Jul 1988
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with acceptable restrictions (no analytics performed)
- Qualifier:
- according to guideline
- Guideline:
- ISO 7346-1 (Determination of the Acute Lethal Toxicity of Substances to a Freshwater Fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)] - Part 2: Semi-static method)
- GLP compliance:
- no
- Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- >= 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- 08 - 12 Jan 1990
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP Guideline study with acceptable restrictions: No analytics of the test substance was performed.
- Qualifier:
- according to guideline
- Guideline:
- ISO 7346-1 (Determination of the Acute Lethal Toxicity of Substances to a Freshwater Fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)] - Part 2: Semi-static method)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 20 Oct- 22 Oct 1986
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- other: DIN 38412/15 German standard method for the examination of water, waste water and sludge; bioassays (group L); determination of the effect of substances in water on fish- fish test (L 15)
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A specific amount of test substance was added directly to the test vessels (filled up to final volumes of 10 L) and the solution stirred for 5 minutes by ultraturrax - Test organisms (species):
- Leuciscus idus
- Details on test organisms:
- TEST ORGANISM
- Common name: Golden orfe
- Length at study initiation: approx. 6 cm
- Feeding during test: no - Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 48 h
- Hardness:
- Approximately 16° dH (about 93 mg Ca/L and 12 mg Mg/L)
- Test temperature:
- 20.0-20.5 °C
- pH:
- 7.7-7.8
- Dissolved oxygen:
- 62-92% air saturation
- Nominal and measured concentrations:
- Nominal: 100, 300, 1000, 3000 and 10,000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: basins containing 10 L test water
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Copper- and chlorine-free drinking water
OTHER TEST CONDITIONS
- Photoperiod: 16 hours light per day
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): mortality at 0, 6, 24 and 48 hours - Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- No mortalities or any sign of abnormal behaviour were reported at any of the concentrations tested
- Sublethal observations / clinical signs:
Table 1. Mortality %
Concentration
(mg/L)
Incubation time (h)
0
6
24
48
100
0
0
0
0
300
0
0
0
0
1000
0
0
0
0
3000
0
0
0
0
10000
0
0
0
0
- Endpoint:
- short-term toxicity to fish
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- supporting study
- Study period:
- 20 Oct- 22 Oct 1986
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- other: DIN 38412/15 German standard method for the examination of water, waste water and sludge; bioassays (group L); determination of the effect of substances in water on fish- fish test (L 15)
- GLP compliance:
- no
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
Referenceopen allclose all
Description of key information
No effects up to the limit of water solubility; read-across.
Key value for chemical safety assessment
Additional information
Since no studies investigating the short-term toxicity of Dub TGI 24 to fish are available, in accordance to Regulation (EC) No 1907/2006 Annex XI, 1.5 a read-across to the structurally related source substances, Glycerides, C16-18 and C18-unsatd. (CAS No. 67701-30-8), Docosanoic acid, ester with 1,2,3-propanetriol (CAS No. 77538-19-3) and Glycerides,C14-18 and C16-18 unsaturated, mono-, di- and tri- (CAS No. 91052-28-7) was conducted. The source substances are representative to evaluate the short-term toxicity of the target substance to fish. The target substance is characterized as a mono, di and triesters with glycerol and 2-decyltetradecanoic acid. The source substances are structurally very similar to the target substance. Glycerides, C16-18 and C18-unsatd. (CAS No. 67701-30-8) is an UVCB substance consisting of C16 – C18 (even) and C18 unsaturated fatty acids with glycerol mainly as triglycerides. Docosanoic acid, ester with 1,2,3-propanetriol (CAS No. 77538-19-3) could be a mono, di and triester mainly with C22 fatty acid and glycerol. Glycerides,C14-18 and C16-18 unsaturated, mono-, di- and tri- (CAS No. 91052-28-7) is characterized as mono, di and triester of glycerol with fatty acids of C14-18 and C16-18 unsaturated.
This read-across is justified in detail in the overall summary (IUCLID Section 6.1) and within the analogue justification in IUCLID Section 13. In this case of read-across, the best suited (highest degree of structural similarity, nearest physico-chemical properties) read-across substance was used for the assessment.
The first study investigated the short-term effects of the source substance Glycerides, C16-18 and C18-unsatd. (CAS 67701-30-8) to freshwater fish. The non-GLP study was performed according to ISO guideline 7346-1 using a semi-static test design. Danio rerio was exposed 96 hours to nominal test substance concentrations of 1000, 3000 and 10000 mg/L. No mortality was observed at any of the tested concentrations and therefore the LC50 (96 h) was determined to be > 10,000 mg/L (nominal).
The second study evaluating the acute toxicity of docosanoic acid, ester with 1,2,3-propanetriol (CAS 77538-19-3) to fish was conducted according to the ISO Guideline 7346-1, under GLP conditions. Danio rerio was exposed to the test substance for 96 hours within a semi-static water regime, at nominal concentrations ranging from 100 to 10000 mg/L. After the exposure period, no mortality was observed in treatment or control groups and therefore, the LC50 (96 h) was determined to be > 10,000 mg/L (nominal).
The third study investigated the short-term toxicity of the source substance Glycerides, C14-18 and C16-18 unsaturated, mono-, di- and tri- (CAS 91052-28-7) to freshwater fish. This test was performed according to the German national Guideline DIN 38412/15 (Examination of water, waste water and sludge; bioassays (L); determination of the effect of substances in water on fish- Fish test (L15)). In this static test, Leuciscus idus were exposed to the test substance for 48 hours. After this period, no mortality or any other effects were reported at any of the concentrations tested (LC50 (48 h) > 10000 mg/L nominal). According to the Guidance on information requirements and chemical safety assessment, chapter R.7b, results obtained at shorter test durations that the standard 96 hours (OECD 203) should be treated with caution and always in combination with other data (read-across, non-testing, etc.). As this study supported the results from both other read across substances, that no short-term effects to fish are expected up to the limit of water solubility, this study is used as supporting study.
Based on the available results from structurally related source substances (in accordance to Regulation (EC) No 1907/2006 Annex XI, 1.5) which are characterized by a similar ecotoxicological profile and comparable structure, it can be concluded that Dub TGI 24 will not exhibit short-term effects to fish up to the limit of water solubility.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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