3-cyano-3,5,5-trimethylcyclohexanone

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1991-03-06 to 1991-03-07

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with acceptable restrictions: No analytical monitoring, duration only 24 hours

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1991

Report date:

1991

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

1984

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3-cyano-3,5,5-trimethylcyclohexanone

EC Number:

411-490-4

EC Name:

3-cyano-3,5,5-trimethylcyclohexanone

Cas Number:

7027-11-4

Molecular formula:

C10H15NO

IUPAC Name:

3-cyano-3,5,5-trimethylcyclohexanone

Details on test material:

Test substance: Isophorone nitrile of Degussa AG. Batch No. 64-II/78 I,90. Purity 99.6 % (GC).

Sampling and analysis

Analytical monitoring:

no

Details on sampling:

- at 0 and 24 hours

Test solutions

Vehicle:

no

Details on test solutions:

STOCK AND TEST SOLUTION AND THEIR PREPARATION
- Dispersion: Accurate weighing and dissolving
- Vehicle, solvent: Dilution water
- Concentration in vehicle/ solvent: 1000 mg/l

STABILITY OF THE TEST CHEMICAL SOLUTIONS: Visually clear solutions

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISMS
- Age: < 24 hours at study initiation
- Feeding during test: No

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

24 h

Test conditions

Test temperature:

- Test temperature: 19.9-20.3 °C

pH:

- pH: 8.0-8.1

Dissolved oxygen:

- Dissolved oxygen: 7.6-8.7 mg/l

Nominal and measured concentrations:

100; 180; 320; 560; 1000 mg/l (nominal concentrations)

Details on test conditions:

DILUTION WATER
- Source: Groundwater from a locality near Linschoten (NL), several salts  added
- Aeration: Only prior to test
- Hardness: 217 mg/l as CaCO3
- TOC: 1.5 mg total inorganic carbon/l
- Ca/Mg ratio: 1.43/0.74 [mmol/l]
- Na/K ratio: 1.33/0.24 [mmol/l]
- 2.79 mmol Cl-/l; 0.74 mmol SO4--/l
- pH: 8.0-8.2

TEST SYSTEM
- Exposure vessel type: 150 ml beaker with 100 ml test solution
- Number of replicates, individuals per replicate: 4 replicates with 5  individuals each
- Control group: dilution water
- Photoperiod: 16 h light / 8 h dark with 2 x 30 min transition period

TEST PARAMETER: 
- Main: Immobility as defined in OECD Guideline 202.  Additionally comparison of visually observable criteria (swimming behavior, color,  others) with control, and microscopic check of all 1000 mg/l and half of the 560 mg/l daphnids.#

SAMPLING: At beginning and end (Temperature, dissolved oxygen, pH, test  parameters)

STATISTICS
- EC50 values and their corresponding confidence intervals were calculated by means of a parametric model developed by Kooijman

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

NOEC

Effect conc.:

320 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: mobility and condition

Effect concentrations 2

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

560 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

827 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 714-956 mg/L

Effect concentrations 4

Duration:

24 h

Dose descriptor:

EC100

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Other effects: 100-320 mg/l: condition similar to control animals; 560 mg/l: slow and irregular movements and lower positions of all mobile 
daphnia, 1000 mg/l: 3 animals dead, 12 immobile, 5 with slow movements

Results with reference substance (positive control):

- no data available

Reported statistics and error estimates:

- Concentration / response curve: Slope 0.17 (95 % confidence interval: 0.08-0.26)

Any other information on results incl. tables

RESULTS
- Effect data (Immobilization): 
  Control:    0/20
   100 mg/l:  0/20
   180 mg/l:  0/20
   320 mg/l:  0/20
   560 mg/l:  2/20
  1000 mg/l: 15/20
  Since 10 % immobilization in the controls is allowed by the test guideline, 560  mg/l with 2/20 immobile daphnia was considered as EC0 by the authors.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The EC50 (24 hours) was calculated to be 827 mg/L indicating that the test substance poses no significant hazard to Daphnia magna.

Executive summary:

The acute toxictiy of isophorone nitrile on Daphnia magna was investigated over a period of 24 hours by TNO (1991) according to OECD 202. Five concentrations ranging from 100 to 1000 mg/L plus controls were tested. The following enpoints were derived:

EC50 (24 hours): 827 mg/L (CL: 714 -956 mg/L)

NOEC (24 hours): 320 mg/L

The study was assessed as "reliable with restrictions".