Thiosemicarbazide

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

OECD 202

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

other: The Notice on the Test Method Concerning New Chemical Substances

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Vehicle:

not specified

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method:Test solutions were prepared by mixing a stock solution of the test substance, which was prepared by dissolving the substance in test water.

Test organisms (species):

Daphnia sp.

Details on test organisms:

TEST ORGANISM
- Feeding during test: No

Test type:

semi-static

Water media type:

other: dechlorinated tap water

Limit test:

no

Total exposure duration:

48 h

Test temperature:

19.9 - 20.1 ºC

Nominal and measured concentrations:

Nominal:30, 20, 13, 8.9, 5.9 mg/L,(geometric ratio 1.5) control

Details on test conditions:

TEST SYSTEM
- Material, size, headspace, fill volume: Test solution volume 400 mL/concentration (100 mL/vessel)
- Aeration: No
- Renewal rate of test solution (frequency/flow rate): After 24 hours of exposure
- No. of organisms per vessel: 5 (20 /concentration)
- No. of vessels per concentration (replicates):4

OTHER TEST CONDITIONS
- Photoperiod: Room light
- Light intensity: 16 hours light / 8 hours dark

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

ca. 12 mg/L

Nominal / measured:

nominal

Conc. based on:

not specified

Basis for effect:

mobility

Remarks on result:

other: 95% CL= 10 - 14mg/L

Details on results:

- Lowest concentration causing 100 % immobility (48 h): > 30 mg/L
- Highest concentration causing 0 % immobility (48 h): 5.9 mg/L
All results were based on the nominal values.

Validity criteria fulfilled:

not specified

Conclusions:

The test item has effective concentration EC50 (48h) of 12mg/L . (Acute toxicity on daphnia magna)

Executive summary:

Based on available data from National instititute of technology and evaluation (NITE) test item was tested to the toxicity to freswater species of Daphnia Magna, in the nominal concentration: 30, 20, 13, 8.9, 5.9 mg/L , 0 mg/L (control) and the temperature 19.9 - 20.1 ºC according the OECD TG 202, followed GLP principles. Test was performed in semi-static condition during 48 hours. Five organism per vessels (20 per concentrations) were used. Test solutions were prepared by mixing a stock solution of the test substance, which was prepared by dissolving the substance in test water.The Effective concentration EC50 (48h) of 12 mg/L based on mobility was evaluated.

Description of key information

Key study: Based on available data from National instititute of technology and evaluation (NITE) test item has Effective concentration EC50 (48h) of 12 mg/L based on mortality.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

12 mg/L

Additional information

Key study:OECD TG 202, followed GLP principles.

Based on available data from National instititute of technology and evaluation (NITE) test item was tested to the toxicity to freswater species of Daphnia Magna, in the nominal concentration: 30, 20, 13, 8.9, 5.9 mg/L , 0 mg/L (control) and the temperature 19.9 - 20.1 ºC . Test was performed in semi-static condition during 48 hours. Test solutions were prepared by mixing a stock solution of the test substance, which was prepared by dissolving the substance in test water.The Effective concentration EC50 (48h) of 12 mg/L based on mobility was evaluated.