2-[2-[4-[(2-cyanoethyl)methylamino]phenyl]vinyl]-1,3,3-trimethyl-3H-indolium hydrogen sulphate

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms, other

Remarks:

Pseudomonas putida respiration inhibition study

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

04 Aug 1987 to 04 Aug 1987

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Qualifier:

no guideline followed

Principles of method if other than guideline:

Oxygen consumption test using Pseudomonas putida by the test method of Robra.

GLP compliance:

no

Specific details on test material used for the study:

- Name of test material (as cited in study report): Basacryl Brilliantrot x 4G
- Batch No.: P. 1/86

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test substance was added to the test water and stirred during 17 hours using a magnetic stirrer and stir bar. The test substance dissolved completely.

Test organisms (species):

Pseudomonas putida

Details on inoculum:

- Age of the bacterial suspension: 24 h
- E (436nm/1cm): 0.380 at 1:100 dilution

Test type:

static

Water media type:

not specified

Limit test:

no

Test temperature:

25 °C

Nominal and measured concentrations:

- Nominal test concentrations: 0 (control), 62.5, 125, 250, 500 and 1000 mg/L
- Test concentrations were not analytically verified.

Reference substance (positive control):

no

Dose descriptor:

EC10

Effect conc.:

62.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks on result:

other: reported as EC0 with 10% tolerance limit

Details on results:

The oxygen consumption rate decreased with exposure concentration in a concentration-dependent manner. See 'Any other information on results incl. tables'.

Table: Oxygen consumption

Concentration (mg/L)	Oxygen consumption (mg/L·min)
	1	2	3	MV	MV %
0 (Blanc control)	2.15	2.20	-	-	-
1000	-	-	-	-	-
500	0.95	0.95	-	0.95	42
250	1.50	1.55	1.60	1.55	68.5
125	2.00	2.05	-	2.025	89.5
62.5	2.15	2.00	-	2.075	91.7
0 (Blanc control)	2.30	2.40	-	2.2625*	-

*mean value over all four blanco control measurements

MV = mean value

Validity criteria fulfilled:

not specified

Additional information

The toxicity of the substance Basacryl-brilliantrot x 4G to microorganisms was investigated in an oxygen consumption test using Pseudomonas putida by the test method of Robra. In this study cultures of bacteria (Pseudomonas putida) were exposed to nominal test substance concentrations of 0 (control), 62.5, 125, 250, 500 and 1000 mg/L. Test concentrations were not analytically verified. Bacterial oxygen consumption rate (mg/L·min) was determined and compared to a blank control. A concentration-dependent decrease in oxygen consumption rate was observed. At a test concentration of 62.5 mg/L 8.3% inhibition of oxygen consumption rate was recorded. At higher test concentrations the inhibition was >10% and therefore the EC0 value with 10% tolerance was determined at 62.5 mg/L. This value may be considered the EC10.