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EC number: 209-940-8 | CAS number: 598-56-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study according to national standards without analytical concentration verification
- Qualifier:
- according to guideline
- Guideline:
- other: German industrial standard test guideline DIN 38412, Part 9
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: green algae, (Scenedesmus subspicatus)
- Strain: SAG 86.81
ACCLIMATION
- Culturing media and conditions: same as test
Erlenmeyer flasks (nominal volume 250 ml) plugged with gas permeable siliconsponge caps
Temperature: 20 °C
Culturing volume: 100 ml - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Test temperature:
- 20 °C
- pH:
- 8.1 -9.4
- Nominal and measured concentrations:
- Nominal concentrations: 0.029, 0.059, 0.117, 0.234, 0.469, 0.938, 1.875, 3.75, 7.5, 15, 30 mg/L. Aditionally a control was tested in parallel.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 10 mL test volume, test vessels not further specified
- Initial cells density: Initial count of algae cells/ml: 10000
- No. of vessels per concentration (replicates): 6; blank per concentration: 1
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes; OECD Medium
Temperature: 20 °C
Culturing volume: 100 ml
TEST MEDIUM / WATER PARAMETERS
Test substance preparation: none
- Culture medium different from test medium: no
- stock solution of test substance: 60 mg/L (pH: 10.5, conductivity: 54 µSiemens/cm); stock solution was diluted in test medium to reach the final test concentrations
OTHER TEST CONDITIONS
- pH: 8.5 ±0.5
- Light intensity and quality: 230/60 µE above /under test vessel, respectively - Reference substance (positive control):
- no
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 24.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CL: 21.9 to 27.3 mg/L; recalculated value
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 11.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CL: 9.33 to 13.4 mg/L; recalculated value
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 17 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: 95% CL: 8.45 to 96.4 mg/L; recalculated value
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 4.08 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Remarks on result:
- other: 95% CL: 1.08 to 6.83 mg/L; recalculated value
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 15.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Remarks on result:
- other: 95% CL: 9.85 to 30.5 mg/L; recalculated value
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 2.52 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: 95% CL: 0.111 to 5.54 mg/L; recalculated value
- Reported statistics and error estimates:
- The test was re-evaluated using ToxRatPro Version 2.09 using the measured fluorescence data which were equated with cell numbers.
- Validity criteria fulfilled:
- not applicable
- Executive summary:
Acute toxicity to algae has been investigated according to German industrial standard test guideline DIN 38412, Part 9.
Algae were exposed for 96 hours to an aqueous solution of ethyldimethylamine at nominal concentrations of 0.029, 0.059, 0.117, 0.234, 0.469, 0.938, 1.875, 3.75, 7.5, 15, 30 mg/L.
ErC 50 - 72h has been estimated based on results obtained and is of 24.2 mg/L and ErC10 of 11.6 mg/L.
Reference
The following nominal effect concentrations
related to yield (EyC), growth rate (ErC), and biomass integral (EbC)
were recalculated according to OECD TG 201 (adopted 23 Mar 2006) using
the fluorescence values for inhibition of biomass from the original
report. Since no calibration curve data were available, fluorescence
data were equated with cell numbers:
Yield (mg/L) |
||
72 h |
96 h |
|
EyC10 |
4.1 |
1.7 |
EyC20 |
6.4 |
2.9 |
EyC50 |
15.4 |
7.9 |
Growth rate (mg/L) |
||
72 h |
96 h |
|
ErC10 |
11.6 |
6.1 |
ErC20 |
14.9 |
9.6 |
ErC50 |
24.2 |
23.2 |
Biomass integral (mg/L) |
||
72 h |
96 h |
|
EbC10 |
2.5 |
3.7 |
EbC20 |
4.9 |
5.7 |
EbC50 |
17.0 |
12.8 |
Original effect values as given in the report (nominal concentrations in mg/L) |
||
EC20 |
0.82 - 9.2 |
0.77 - 3.0 |
EC50 |
19.6 |
7.6 |
EC90 |
> 30 |
29.6 |
Cell growth inhibition
Treatment [mg/L] |
Growth rate inhibition [%] |
Biomass decrease [%] |
Yield Decrease [%] |
Control |
0 |
0 |
0.0 |
0.029 |
4.2 |
15.9 |
12.7 |
0.059 |
-1.0 |
5.4 |
4.2 |
0.117 |
1.8 |
7.9 |
6.9 |
0.234 |
0.2 |
6.9 |
3.4 |
0.469 |
2.5 |
11.2 |
10.0 |
0.938 |
9.2 |
21.9 |
25.1 |
1.875 |
-0.2 |
4.8 |
-0.8 |
3.750 |
0 |
7.7 |
3.7 |
7.500 |
4.0 |
14.5 |
16.7 |
15.000 |
16.1 |
29.3 |
37.1 |
30.000 |
66.2 |
90.7 |
90.2 |
pH values
Concentration [mg/L] |
uninoculated |
inoculated |
|
0 h |
96 h |
96 h |
|
Control |
8.5 |
8.3 |
9.0 |
0.029 |
8.6 |
8.2 |
9.0 |
0.059 |
8.6 |
8.1 |
9.1 |
0.234 |
8.6 |
8.1 |
9.1 |
0.469 |
8.6 |
8.1 |
9.0 |
0.938 |
8.6 |
8.1 |
9.0 |
1.875 |
8.6 |
8.1 |
9.0 |
3.75 |
8.7 |
8.1 |
8.8 |
7.5 |
8.8 |
8.1 |
8.5 |
15 |
9.1 |
8.1 |
8.4 |
30 |
9.4 |
8.1 |
8.3 |
Description of key information
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 24.2 mg/L
- EC10 or NOEC for freshwater algae:
- 11.6 mg/L
Additional information
Ehyldimethylamine toxicity to green algae has been investigated by BASF in 1988. The methodology followed is the DIN 38412, Part 9. with exposure duration exceeding 72 h (96h). In this context, the extrapolated 72 h ErC50 determined is of 24.2 mg/L and a 72 h ErC10 of 11.6 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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