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EC number: 616-628-9 | CAS number: 78543-37-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- July 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 997
- Report date:
- 1997
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- 1996-21-01
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- potassium dibenzyl phosphate
- EC Number:
- 616-628-9
- Cas Number:
- 78543-37-0
- Molecular formula:
- C14-H14-O4-P.K
- IUPAC Name:
- potassium dibenzyl phosphate
- Test material form:
- solid
- Details on test material:
- Batch 97/8
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102
- Metabolic activation:
- with and without
- Test concentrations with justification for top dose:
- Dose: 0, 50, 150, 500, 1500, 5000 µg/plate
At 5000 µg/plate the test material caused no visible reduction in the growth of the bacterial lawn at any of the dose levels to anyof the strains of Salmonella tested. - Vehicle / solvent:
- Distilled water
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- 4-nitroquinoline-N-oxide
- 9-aminoacridine
- N-ethyl-N-nitro-N-nitrosoguanidine
- mitomycin C
- other: 2-Aminoanthracene, 1,8-dihydroxyanthroquinone
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in medium; in agar (plate incorporation)
DURATION
- Preincubation period:
- Exposure duration: 48-hour incubation at 37°C
NUMBER OF REPLICATIONS:
Duplicate in the preliminary study
Triplicate in the main test
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 102
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
Any other information on results incl. tables
Experiment 1 – Without metabolic activation
with or Without S9-mix |
concentration µg/plate |
Nimber of revertants (Number of colonies per plate) |
|||||||||
Base-pair Substitution tuype |
Frameshift type |
||||||||||
TA100 |
TA1535 |
TA102 |
TA98 |
TA1537 |
|||||||
- |
0 |
66 |
|
12 |
|
219 |
|
21 |
|
7 |
|
|
(67) |
|
(15) |
|
(220) |
|
(19) |
|
(9) |
||
73 |
|
14 |
|
226 |
|
24 |
|
13 |
|
||
|
5.6 |
|
3.1 |
|
6.0 |
|
5.7 |
|
3.8 |
||
62 |
|
18 |
|
214 |
|
13 |
|
6 |
|
||
- |
50 |
76 |
|
16 |
|
224 |
|
14 |
|
5 |
|
|
(72) |
|
(16) |
|
(224) |
|
(15) |
|
(11) |
||
70 |
|
20 |
|
218 |
|
17 |
|
16 |
|
||
|
3.8 |
|
3.5 |
|
5.5 |
|
2.1 |
|
5.5 |
||
69 |
|
13 |
|
229 |
|
13 |
|
11 |
|
||
- |
150 |
65 |
|
11 |
|
20 |
|
15 |
|
7 |
|
|
(71) |
|
(14) |
|
(223) |
|
(18) |
|
(11) |
||
75 |
|
18 |
|
220 |
|
16 |
|
12 |
|
||
|
5.3 |
|
3.5 |
|
5.8 |
|
4.4 |
|
3.6 |
||
73 |
|
14 |
|
230 |
|
23 |
|
14 |
|
||
- |
500 |
61 |
|
18 |
|
229 |
|
15 |
|
8 |
|
|
(66) |
|
(18) |
|
(226) |
|
(18) |
|
(11) |
||
68 |
|
15 |
|
215 |
|
20 |
|
11 |
|
||
|
6.0 |
|
3.0 |
|
9.5 |
|
2.5 |
|
3.6 |
||
68 |
|
21 |
|
233 |
|
18 |
|
11 |
|
||
- |
1500 |
72 |
|
11 |
|
213 |
|
12 |
|
10 |
|
|
(66) |
|
(14) |
|
(219) |
|
(12) |
|
(9) |
||
66 |
|
17 |
|
226 |
|
10 |
|
10 |
|
||
|
6.0 |
|
3.0 |
|
6.5 |
|
2.0 |
|
1.7 |
||
60 |
|
14 |
|
219 |
|
14 |
|
7 |
|
||
- |
5000 |
65 |
|
15 |
|
218 |
|
17 |
|
10 |
|
|
(66) |
|
(16) |
|
(225) |
|
(16) |
|
(7) |
||
71 |
|
20 |
|
235 |
|
18 |
|
4 |
|
||
|
6.0 |
|
3.6 |
|
8.9 |
|
3.2 |
|
3.0 |
||
63 |
|
13 |
|
222 |
|
12 |
|
7 |
|
||
Positive Controls S9-mix - |
Name |
ENNG |
ENNG |
MMC |
4NQO |
9AA |
|||||
Concentration (µg/plate |
3 |
5 |
0.5 |
0.2 |
80 |
||||||
N° colonies per plate |
890 |
|
791 |
|
529 |
|
247 |
|
874 |
|
|
|
(863) |
|
(825) |
|
(576) |
|
(218) |
|
(800) |
||
797 |
|
882 |
|
618 |
|
199 |
|
802 |
|
||
|
57.1 |
|
49.9 |
|
25.5 |
|
25.5 |
|
75.0 |
||
901 |
|
801 |
|
582 |
|
208 |
|
724 |
|
ENNG N-ethyl-N’-nitro-N-nitrosoguanidine
MMC Mitomycin C
4NQO 4-nitroquinoline-1-oxide
9AA 9-aminoacridine
Experiment 1 – With metabolic activation
with or Without S9-mix |
concentration µg/plate |
Nimber of revertants (Number of colonies per plate) |
|||||||||
Base-pair Substitution tuype |
Frameshift type |
||||||||||
TA100 |
TA1535 |
TA102 |
TA98 |
TA1537 |
|||||||
+ |
0 |
71 |
|
14 |
|
216 |
|
17 |
|
8 |
|
|
’76) |
|
(14) |
|
(221) |
|
(18) |
|
(13) |
||
81 |
|
19 |
|
226 |
|
24 |
|
17 |
|
||
|
5.0 |
|
4.5 |
|
5.0 |
|
5.1 |
|
4.7 |
||
76 |
|
10 |
|
222 |
|
14 |
|
15 |
|
||
+ |
50 |
77 |
|
13 |
|
224 |
|
14 |
|
9 |
|
|
(74) |
|
(13) |
|
(226) |
|
(16) |
|
(12) |
||
82 |
|
16 |
|
236 |
|
15 |
|
11 |
|
||
|
9.8 |
|
3.0 |
|
9.6 |
|
2.1 |
|
4.2 |
||
63 |
|
10 |
|
217 |
|
18 |
|
17 |
|
||
+ |
150 |
68 |
|
12 |
|
224 |
|
11 |
|
10 |
|
|
(75) |
|
(14) |
|
(217) |
|
(12) |
|
(12) |
||
77 |
|
17 |
|
217 |
|
10 |
|
16 |
|
||
|
5.9 |
|
1.5 |
|
6.5 |
|
3.2 |
|
3.8 |
||
81 |
|
10 |
|
211 |
|
16 |
|
9 |
|
||
+ |
500 |
68 |
|
14 |
|
213 |
|
12 |
|
9 |
|
|
(76) |
|
(15) |
|
(219) |
|
(18) |
|
(12) |
||
79 |
|
15 |
|
228 |
|
22 |
|
12 |
|
||
|
2.3 |
|
2.9 |
|
7.9 |
|
5.1 |
|
2.5 |
||
70 |
|
2 |
|
216 |
|
19 |
|
14 |
|
||
+ |
1500 |
75 |
|
13 |
|
214 |
|
17 |
|
14 |
|
|
(75) |
|
(13) |
|
(221) |
|
(16) |
|
(14) |
||
79 |
|
81 |
|
228 |
|
15 |
|
17 |
|
||
|
10.4 |
|
4.2 |
|
7.0 |
|
1.0 |
|
3.0 |
||
75 |
|
13 |
|
220 |
|
16 |
|
11 |
|
||
+ |
5000 |
63 |
|
12 |
|
214 |
|
20 |
|
7 |
|
|
|
|
|
|
(220) |
|
(19) |
|
(11) |
||
82 |
|
10 |
|
219 |
|
21 |
|
11 |
|
||
|
|
|
|
|
6.6 |
|
3.2 |
|
3.5 |
||
80 |
|
18 |
|
227 |
|
15 |
|
14 |
|
||
Positive Controls S9-mix - |
Name |
2AA |
2AA |
DANTHRON |
2AA |
2AA |
|||||
Concentration (µg/plate |
1 |
2 |
10 |
0.5 |
2 |
||||||
N° colonies per plate |
1045 |
|
325 |
|
510 |
|
386 |
|
321 |
|
|
|
(1046) |
|
(302) |
|
(543) |
|
(312) |
|
(329) |
||
1121 |
|
288 |
|
503 |
|
259 |
|
350 |
|
||
|
74.0 |
|
19.9 |
|
62.7 |
|
65.9 |
|
18.7 |
||
973 |
|
294 |
|
615 |
|
292 |
|
315 |
|
2AA: 2-aminoanthracene
DANTHRON: 1.8-dihydroxyanthraquinone
Experiment 2 – Without metabolic activation
with or Without S9-mix |
concentration µg/plate |
Nimber of revertants (Number of colonies per plate) |
|||||||||
Base-pair Substitution tuype |
Frameshift type |
||||||||||
TA100 |
TA1535 |
TA102 |
TA98 |
TA1537 |
|||||||
- |
0 |
101 |
|
26 |
|
271 |
|
17 |
|
11 |
|
|
(99) |
|
(22) |
|
(270) |
|
(20) |
|
(8) |
||
96 |
|
21 |
|
277 |
|
20 |
|
6 |
|
||
|
2.9 |
|
3.6 |
|
7.0 |
|
3.0 |
|
2.5 |
||
101 |
|
19 |
|
263 |
|
23 |
|
8 |
|
||
- |
50 |
91 |
|
21 |
|
264 |
|
20 |
|
5 |
|
|
(93) |
|
(25) |
|
(274) |
|
(18) |
|
(8) |
||
78 |
|
29 |
|
270 |
|
16 |
|
9 |
|
||
|
16.6 |
|
4.0 |
|
12.5 |
|
2.0 |
|
2.3 |
||
111 |
|
26 |
|
288 |
|
18 |
|
9 |
|
||
- |
150 |
73 |
|
20 |
|
218 |
|
20 |
|
11 |
|
|
(81) |
|
(22) |
|
(232) |
|
(19) |
|
(9) |
||
93 |
|
29 |
|
262 |
|
19 |
|
7 |
|
||
|
10.6 |
|
6.2 |
|
25.7 |
|
1.0 |
|
2.1 |
||
77 |
|
17 |
|
217 |
|
18 |
|
8 |
|
||
- |
500 |
92 |
|
23 |
|
228 |
|
18 |
|
10 |
|
|
(94) |
|
(21) |
|
(233) |
|
(19) |
|
(10) |
||
99 |
|
18 |
|
254 |
|
23 |
|
14 |
|
||
|
4.7 |
|
2.9 |
|
19.4 |
|
3.6 |
|
3.5 |
||
90 |
|
23 |
|
216 |
|
16 |
|
7 |
|
||
- |
1500 |
109 |
|
25 |
|
264 |
|
22 |
|
15 |
|
|
(101) |
|
(24) |
|
(271) |
|
(21) |
|
(11) |
||
91 |
|
25 |
|
286 |
|
19 |
|
11 |
|
||
|
9.2 |
|
2.9 |
|
13.3 |
|
2.1 |
|
3.5 |
||
103 |
|
23 |
|
262 |
|
23 |
|
8 |
|
||
- |
5000 |
105 |
|
24 |
|
287 |
|
18 |
|
9 |
|
|
(89) |
|
(24) |
|
(255) |
|
(17) |
|
(10) |
||
74 |
|
24 |
|
244 |
|
12 |
|
9 |
|
||
|
15.5 |
|
0.6 |
|
28.5 |
|
4.2 |
|
1.2 |
||
89 |
|
25 |
|
233 |
|
20 |
|
11 |
|
||
Positive Controls S9-mix - |
Name |
ENNG |
ENNG |
MMC |
4NQO |
9AA |
|||||
Concentration (µg/plate |
3 |
5 |
0.5 |
0.2 |
80 |
||||||
N° colonies per plate |
1083 |
|
1088 |
|
595 |
|
213 |
|
742 |
|
|
|
(1017) |
|
(1052) |
|
(588) |
|
(228) |
|
(785) |
||
922 |
|
1115 |
|
544 |
|
197 |
|
708 |
|
||
|
84.3 |
|
86.8 |
|
41.0 |
|
41.2 |
|
105.9 |
||
1046 |
|
953 |
|
625 |
|
275 |
|
906 |
|
ENNG N-ethyl-N’-nitro-N-nitrosoguanidine
MMC Mitomycin C
4NQO 4-nitroquinoline-1-oxide
9AA 9-aminoacridine
Experiment 2 – With metabolic activation
with or Without S9-mix |
concentration µg/plate |
Nimber of revertants (Number of colonies per plate) |
|||||||||
Base-pair Substitution tuype |
Frameshift type |
||||||||||
TA100 |
TA1535 |
TA102 |
TA98 |
TA1537 |
|||||||
+ |
0 |
103 |
|
14 |
|
272 |
|
28 |
|
10 |
|
|
(108) |
|
(13) |
|
(276) |
|
(27) |
|
(10) |
||
101 |
|
15 |
|
279 |
|
30 |
|
8 |
|
||
|
9.9 |
|
2.1 |
|
3.5 |
|
3.6 |
|
2.5 |
||
119 |
|
11 |
|
276 |
|
23 |
|
13 |
|
||
+ |
50 |
117 |
|
17 |
|
284 |
|
31 |
|
7 |
|
|
(114) |
|
(18) |
|
(273) |
|
(29) |
|
(8) |
||
103 |
|
23 |
|
256 |
|
29 |
|
11 |
|
||
|
9.8 |
|
5.0 |
|
14.7 |
|
2.0 |
|
2.3 |
||
122 |
|
13 |
|
278 |
|
27 |
|
7 |
|
||
+ |
150 |
120 |
|
10 |
|
272 |
|
29 |
|
15 |
|
|
(118) |
|
(13) |
|
(253) |
|
(32) |
|
(12) |
||
112 |
|
16 |
|
249 |
|
29 |
|
11 |
|
||
|
4.9 |
|
3.0 |
|
17.3 |
|
4.6 |
|
3.1 |
||
121 |
|
13 |
|
238 |
|
37 |
|
9 |
|
||
+ |
500 |
117 |
|
25 |
|
275 |
|
24 |
|
10 |
|
|
(113) |
|
(18) |
|
(274) |
|
(22) |
|
(10) |
||
113 |
|
16 |
|
281 |
|
25 |
|
8 |
|
||
|
4.0 |
|
6.2 |
|
8.1 |
|
4.9 |
|
2.5 |
||
109 |
|
13 |
|
265 |
|
16 |
|
13 |
|
||
+ |
1500 |
113 |
|
15 |
|
291 |
|
19 |
|
8 |
|
|
(121) |
|
(14) |
|
(282) |
|
(23) |
|
(8) |
||
129 |
|
16 |
|
282 |
|
25 |
|
8 |
|
||
|
8.0 |
|
2.1 |
|
9.5 |
|
3.8 |
|
0.0 |
||
120 |
|
12 |
|
272 |
|
26 |
|
8 |
|
||
+ |
5000 |
111 |
|
15 |
|
280 |
|
25 |
|
13 |
|
|
(112) |
|
(14) |
|
(278) |
|
(27) |
|
(10) |
||
128 |
|
10 |
|
271 |
|
26 |
|
8 |
|
||
|
15.5 |
|
3.2 |
|
6.2 |
|
2.6 |
|
2.5 |
||
97 |
|
16 |
|
283 |
|
30 |
|
10 |
|
||
Positive Controls S9-mix - |
Name |
2AA |
2AA |
DANTHRON |
2AA |
2AA |
|||||
Concentration (µg/plate |
1 |
2 |
10 |
0.5 |
2 |
||||||
N° colonies per plate |
1060 |
|
347 |
|
523 |
|
483 |
|
171 |
|
|
|
(1038) |
|
(329) |
|
(556) |
|
(471) |
|
(197) |
||
1135 |
|
315 |
|
622 |
|
506 |
|
216 |
|
||
|
109.7 |
|
16.5 |
|
56.9 |
|
42.3 |
|
23.2 |
||
919 |
|
324 |
|
524 |
|
424 |
|
203 |
|
2AA: 2-aminoanthracene
DANTHRON: 1.8-dihydroxyanthraquinone
Applicant's summary and conclusion
- Conclusions:
- The Dibenzyl Potassium Phosphate was considered to be not genotoxic in an Ames test.
- Executive summary:
The genotoxicty od Dibenzyl Potassium Phosphate has been determined according to OECD 471 test guideline ijn compliance with GLP
In this test, the substance was determinated to be non- genotoxic.
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