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EC number: 814-233-8 | CAS number: 444649-70-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From February 22, 2016 to March 22, 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Batch no.: JBGJ0045R
Purity: 100% (UVCB)
Appearance: clear yellowish liquid - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- - Specification: cctivated sludge from a biologic sewage treatment plant was used. The chosen plant is treating mostly domestic sewage.
- Source and pre-treatment: Source: the sludge was taken from the activation basin of the ESN (Stadtentsorgung Neustadt) sewage treatment plant, Im Altenschemel, NW-Lachen-Speyerdorf. Date of collection: 19. Feb. 2016, batch no: 20160219.
- Pre-treatment: the sludge was filtrated, washed with tap water (2x), then washed with and re-suspended in test medium. It was then aerated until use. The dry matter was determined as 4060 mg suspended solids/L. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 31.3 mg/L
- Based on:
- other: nominally 20 mg organic carbon/L
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- - The medium was prepared from the stock solutions. The stock solution of the positive control was p repared and its TOC was measured. The inoculum was taken from its source, washed, aerated and the d ry matter was determined. The test vessels were filled with medium and inoculum. Then, all flasks were aerated for 96 hours with purified, CO2-free, moistened air to purge the system of CO2. On the day of the start of the test, CO2-free medium and inoculum was filled into the test flask.
- Experimental parameters:
Flask volume: 1500 mL
Apparatus blanks: 2, containing mineral medium only
Blank controls: 2, containing mineral medium and inoculum
Positive control flasks: 2, containing positive control, mineral medium and inoculum
Test flasks: 2, containing test substance, mineral medium and inoculum
Abiotic: control 1, containing test substance, mineral medium and HgCl2
Toxicity control: 1, containing test substance, positive control, mineral medium and inoculum Inoculum concentration: 25.0 mg/L
Temperature: 19.1 – 21.6°C
Duration: 28 days
The test was performed with a nominal start concentration of 20 mg organic carbon/L.
- The emitted CO2 was trapped in 0.25 M NaOH. Two scrubbers containing 100 mL each were connected in series to the test vessels. The initial IC value of the 0.25 M NaOH was separately determined in each flask.
- Sampling: from each front scrubber flask, 10 samples were taken in order to determine the emitted CO2 (on day 0, 2, 4, 7, 9, 11, 14, 18, 23 and 29). The sample volume was 1 mL. The resulting change in th e volume of the front flask was considered in the calculation of emitted CO2. On day 28, 5 mL HCl 2 M was added to each test flask in order to drive off dissolved CO2. On day 29, samples from both scrubber flasks were taken.
- CO2 determination: analyses of the emitted CO2 were made by IC measurement using the carbon analyser TOC. - Reference substance:
- aniline
- Remarks:
- positive control
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Remarks:
- made by IC measurement
- Value:
- 36
- Sampling time:
- 28 d
- Remarks on result:
- other: not readily biodegradable (but no plateau was reached by the end of the test indicating possible further degradation)
- Remarks:
- inherently biodegradable
- Details on results:
- The following data were determined for the test substance:
- 10-day-window (day 13 – 23): 27%
- Degradation at the end of the test: 36%
(pass level following guideline: 60% at the end of 10-day-window for pure substances respective 60% at the end of the test for mixtures).
Because the test substance is a mixture the 10-day-window has not to be taken into account. As degradation missed 60% in the course of the test, the test substance was considered as not readily biodegradable within 28 days. However, at the end of the test no plateau of degradation was reached yet, indicating further biodegradation is possible. - Results with reference substance:
- The degradation of the positive control was 69% after 11 days.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- Under the study conditions,the substance was considered as 'not readily biodegradable' in a CO2 evolution test. Nevertheless, considering that no plateau of degradation was reached at the end of 28 days, further degradation is still possible.
- Executive summary:
A study was conducted to determine the biodegradation in water of the test substance, according to OECD guideline 301 B and EU Method C.4-C (CO2 evolution test), in compliance with GLP. The substance was tested in duplicate at the concentration of 20 mg organic carbon/L (corresponding to 31.3 mg test substance/L) for a duration of 28 d. Test and reference substances (positive control: aniline and toxicity control; test substance plus aniline) were added to the bottles containing the microbial organisms (domestic sludge) and mineral components followed by sampling on Day 0, 3, 6, 8, 10, 14, 17, 23 and 29 to measure the CO2 evolution. The amount of CO2 produced was determined by inorganic carbon (IC) measurement using the carbon analyser TOC. Each sample was measured in duplicate or triplicate depending upon the variation.The test substance revealed 36% biodegradation (based on IC) in the duplicate test bottles. No toxicity of the test substance was observed in the toxicity control at a concentration of 39.7 mg/L; i.e., there was 33% degradation after 14 d. Abiotic degradation was not observed. Degradation of the positive control was 69% after 11 d. All validity criteria were met. Under the study conditions, the substance was considered as 'not readily biodegradable' in a CO2 evolution test. Nevertheless, considering that no plateau of degradation was reached at the end of 28 days, further degradation is still possible (Muckle, 2016). Therefore, the test substance has been considered to be inherently biodegradable.
Reference
Description of key information
The test substance has been considered to be inherently biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- inherently biodegradable
Additional information
A study was conducted to determine the biodegradation in water of the test substance, according to OECD Guideline 301 B and EU Method C.4-C (CO2 evolution test), in compliance with GLP. The substance was tested in duplicate at the concentration of 20 mg organic carbon/L (corresponding to 31.3 mg test substance/L) for a duration of 28 d. Test and reference substances (positive control: aniline and toxicity control; test substance plus aniline) were added to the bottles containing the microbial organisms (domestic sludge) and mineral components followed by sampling on Day 0, 3, 6, 8, 10, 14, 17, 23 and 29 to measure the CO2 evolution. The amount of CO2 produced was determined by inorganic carbon (IC) measurement using the carbon analyser TOC. Each sample was measured in duplicate or triplicate depending upon the variation.The test substance revealed 36% biodegradation (based on IC) in the duplicate test bottles. No toxicity of the test substance was observed in the toxicity control at a concentration of 39.7 mg/L; i.e., there was 33% degradation after 14 d. Abiotic degradation was not observed. Degradation of the positive control was 69% after 11 d. All validity criteria were met. Under the study conditions, the substance was considered as 'not readily biodegradable' in a CO2 evolution test. Nevertheless, considering that no plateau of degradation was reached at the end of 28 days, further degradation is still possible (Muckle, 2016). Therefore, the test substance has been considered to be inherently biodegradable.
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