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EC number: 254-296-3 | CAS number: 39108-47-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Read across from GLP compliant guideline study (RL1) with supporting substance (structural analogue)
- Justification for type of information:
- Please refer to section "Additional Information" in the Endpoint Summary for "Read Across Justification".
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 30 min
- Dose descriptor:
- EC10
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Key result
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 372 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 30 min
- Dose descriptor:
- EC10
- Effect conc.:
- > 372 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1999-05-12
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Qualifier:
- according to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- GLP compliance:
- yes
- Specific details on test material used for the study:
- - CAS number: 28141-13-1
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- The test solution was prepared by direct addition of test substance to the test medium.
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- Activated sludge from laboratory wastewater plants treating municipal and synthetic sewage. The inoculum was washed, brought to a concentration of 5 g/L dry substance and aerated for 24 hours. 50 mL were added to a total volume of 250 mL to obtain a concentration of 1 g/L dry substance in the test.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 30 min
- Test temperature:
- 20 ± 2 °C
- pH:
- - Before correction: 3.4
- After correction: 7.6
- After 30 min incubation: 8.0 - Dissolved oxygen:
- - During aeration: >2.5 mg/L
- Immediately before measurement: >6.5 mg/L - Nominal and measured concentrations:
- - Nominal concentrations: 0 (control) and 1000 mg/L
- Measured concentrations: no analytics performed. - Details on test conditions:
- TEST DESIGN:
- Test vessels: Erlenmeyer-vessels (nominal volume 250 mL)
- Test volume: 250 mL
TEST MEDIUM / WATER PARAMETERS:
- Synthetic medium: 8 mL/vessel 100-fold concentrated OECD Medium - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 30 min
- Dose descriptor:
- EC10
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Key result
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 372 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 30 min
- Dose descriptor:
- EC10
- Effect conc.:
- > 372 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- Change in oxygen consumption rate compared with the blank control was 0%.
- Results with reference substance (positive control):
- 30-min EC50: ca. 10 mg/L
- Validity criteria fulfilled:
- yes
Referenceopen allclose all
Description of key information
Based on data available for the close structural analogue 1-Ethyl-2-hydroxy-3-cyan-4-methylpyridon-6 (CAS 28141-13-1), the 30-min EC50 and EC10 values are >372 mg/L (nominal; based on a.i.) in activated sludge.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 372 mg/L
- EC10 or NOEC for microorganisms:
- 372 mg/L
Additional information
No experimental data on the toxicity to microorganisms are available for the substance itself. Therefore, the microorganisms toxicity potential is assessed based on data available for the close structural analogue 1-Ethyl-2-hydroxy-3-cyan-4-methylpyridon-6 (CAS 28141-13-1). This analogue differs from the substance only in that it has an ethyl- instead of a butyl-chain attached to the nitrogen atom in the pyridine ring.
The toxicity of the close structural analogue 1-Ethyl-2-hydroxy-3-cyan-4-methylpyridon-6 (CAS 28141-13-1) to microorganisms was investigated in an activated sludge respiration inhibition study according to OECD guideline No 209 and in compliance with GLP criteria (BASF AG, 1999). In this limit test activated sludge from laboratory wastewater plants treating municipal and synthetic sewage was exposed to nominal test substance concentrations of 0 (control) and 1000 mg/L (based on test substance) for 30 minutes. Test concentrations were not analytically verified. After the 30 minutes incubation period, the change in oxygen consumption rate compared with the blank control was 0%. Based on these findings, the 30-min EC50 and EC10 values are determined at >372 mg/L (nominal, based on a.i.).
As in the read-across study no effects are observed up to the highest concentration tested, the effect values are not corrected for molecular weight. As the target chemical has a slightly higher molecular weight, this may be considered a worst case approach.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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