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EC number: 257-827-7 | CAS number: 52301-70-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Specific investigations: other studies
Administrative data
- Endpoint:
- methaemoglobinaemia
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Start of experiment: 12 March 1981 - End of experiment: 13 March 1981
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Pre GLP study, conducted according to sound scientific principles
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 981
- Report date:
- 1981
Materials and methods
Test guideline
- Qualifier:
- no guideline available
- GLP compliance:
- no
- Type of method:
- in vivo
- Endpoint addressed:
- other: To investigate the Methaemoglobin-inducing capacity of the substance
Test material
- Reference substance name:
- Hexasodium 2,2'-[vinylenebis[(3-sulphonato-4,1-phenylene)imino[6-morpholino-1,3,5-triazine-4,2-diyl]imino]]bis(benzene-1,4-disulphonate)
- EC Number:
- 257-827-7
- EC Name:
- Hexasodium 2,2'-[vinylenebis[(3-sulphonato-4,1-phenylene)imino[6-morpholino-1,3,5-triazine-4,2-diyl]imino]]bis(benzene-1,4-disulphonate)
- Cas Number:
- 52301-70-9
- Molecular formula:
- C40H40N12O20S6.6Na
- IUPAC Name:
- hexasodium 2,2'-[vinylenebis[(3-sulphonato-4,1-phenylene)imino[6-morpholino-1,3,5-triazine-4,2-diyl]imino]]bis(benzene-1,4-disulphonate)
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: Tif: RAIf (SPF) strain
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- Young adult rats of both sexes (Tif: RAIf (SPF) strain) weighing 200-250 g, bred and raised on the premises were used in the experiment. The rats were identified by individual tail and cage marking. They were maintained in groups of two in Macrolon Cages, Type 3 (Ehret, 783 Emmendingen / Germany) with wire mesh tops and saw dust bedding (granular form).
The animal room had a temperature of 22 ± 2°C with 55 ± 10% relative humidity and a 10 hour/day light cycle.
Rat chow No. 890 (NAFAG, Gossau SG) and water were available ad libitum. The adaptation period was at least 7 days.
Test Material and Formulation:
The substance was dissolved in tap water in order to achieve a dosage level of 20 ml/kg body weight. Prior to the administration the formulation was homogeneously dispersed with an Ultra-Turrax and subsequently placed on a magnetic stirrer.
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- water
- Details on exposure:
- Prior to the treatment the animals were fasted overnight. The formulated test compound was administered orally by gavage at a dosage of 5000 mg/kg. Symptoms and mortality were assessed 1, 2, 4, 6 and 24 hours after the administration.
- Analytical verification of doses or concentrations:
- no
- Duration of treatment / exposure:
- See above
- Frequency of treatment:
- See above
Doses / concentrations
- Remarks:
- Doses / Concentrations:
5000 mg/kg
Basis:
Examinations
- Examinations:
- Haematological investigations:
Blood sampling: Blood was collected by puncture of the retroorbital plexus using heparlnized micro-haematocrit tubes at the following times:
prior to the administration (all animals);
- 15, 30, 60, 90 minutes, 2, 4, 6 and 24 hours (each time hal f group).
The blood samples were immediately cooled and the haematological investigation was performed without delay.
Methods used in Haematology:
Haemoglobin and Methaemogl obin was determined with a IL 282 C0-0XYMETER and expressed as percentage of Haemoglobin. - Positive control:
- The identical test as described above was performed with a negative control group of 16 rats, which received 20 ml/kg of tap water and a positive control group which received 40 mg/kg sodium nitrite solved in tap water, administered at a rate of 20 ml/kg.
Results and discussion
- Details on results:
- One animal died immediately after the administration of FAT 65'037/D. Clinical signs of including sedation, dyspnea, ruffled fur, diarrhea and curved body position from moderate to severe were noted in all animals.
Applicant's summary and conclusion
- Conclusions:
- The substance has been found to be devoid of Methaemoglobin-inducing capacity after single oral administration of 5000 mg/kg in young adult rats.
- Executive summary:
The purpose of the present study was to Methaemoglobin-inducing capacity of the substance comparison with a negative control (tap control group (sodium nitrite). The substance has been found to be devoid of Methaemoglobin-inducing capacity after single oral administration of 5000 mg/kg in young adult rats.
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