4-ethyl-2,3-dioxopiperazine-1-carbonyl chloride

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

secondary literature

Justification for type of information:

Data is from study report

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Principles of method if other than guideline:

Acute oral toxicity study of Diethylcarbamoyl chloride in rat

GLP compliance:

not specified

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

diethylcarbamic chloride

Cas Number:

88-10-8

Molecular formula:

C5H10ClNO

IUPAC Name:

diethylcarbamic chloride

Test material form:

liquid

Details on test material:

- Name of test material (as cited in study report): Diethylcarbamoyl chloride
- Molecular formula: C5H10ClNO
- Molecular weight: 135.59 g g/mol
- Substance type: Organic
- Physical state: Liquid

Specific details on test material used for the study:

- Name of test material (as cited in study report): Diethylcarbamoyl chloride
- Molecular formula (if other than submission substance): C5H10ClNO
- Molecular weight (if other than submission substance): 135.593 g/mole
- Substance type: Organic

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

No data available

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

unchanged (no vehicle)

Details on oral exposure:

DOSAGE PREPARATION (if unusual): Compound administered undiluted as a single dose in 2000, 2350, 2600 and 2900 mg/kg bw

Doses:

2000, 2350, 2600 and 2900 mg/kg bw

No. of animals per sex per dose:

Total: 20
2000 mg/kg bw: 2 male, 3 female
2350 mg/kg bw: 3 male, 2 female
2600 mg/kg bw: 4 male, 1 female
2900 mg/kg bw: 5 female

Control animals:

no

Details on study design:

- Duration of observation period following administration: No data
- Frequency of observations and weighing: No data
- Necropsy of survivors performed: yes
- Other examinations performed: Mortality and histopathology were examined.

Statistics:

After approximate MLD was found. Group of rat were dosed at level designed to higher +tv toxicity rang thereby supplying data for calculation of the LD50.

Results and discussion

Preliminary study:

No data available

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

2 700 mg/kg bw

Based on:

test mat.

Remarks on result:

other: 50 % mortality observed

Mortality:

When treated with 2900 mg/kg bw, all the five female rats were died. Half of deaths occurred in 30 minutes to two hours while the other occurred in 24 to 72 hours.
When treated with 2600 mg/kg bw, 1 male and 1 female rat died
When treated with 2350 mg/kg bw, 1 male rat died.
When treated with 2000 mg/kg bw, No mortality were observed in treated male and female rats.

Clinical signs:

other: Muscular in coordination, lethargy, convulsions and coma were observed in died rats, Moderately severe diarrhea was observed in all surviving rats.

Gross pathology:

Alteration In Gastric Secretion, severally irritant intestinal mucosa, abnormally dark liver and greenish colure spleen was observed in treated rats.

Other findings:

Hemorrhogic area in Liver and congestion of tubule in kidney were observed in treated rats.

Any other information on results incl. tables

The Acute Oral Toxicity (LD50) of Diethylcarbamoyl Chloride for rats

Animal No.- Sex	Weight in gm	Doses g/kg	Data
1.Male	220	2.00	Survived
2. Male	250		Survived
3. Female	195		Survived
4. Female	18S		Died
5. Female	210		Survived
6. Male	230	2.35	Survived
7 Male	245		Died
8 Male	235		Survived
9 Female	200		Survived
10 Female	210		Survived
11. Female	180	2.60	Died
12. Male	195		Survived
13. Male	230		Died
14. Male	215		Survived
15. Male	200		Survived
16. Female	175	2.90	Died
17. Female	190		Died
18 Female	180		Died
19. Female	205		Died
20. Female	220		Died

Applicant's summary and conclusion

Interpretation of results:

Category 5 based on GHS criteria

Conclusions:

LD50 was estimated to 2700 mg/kg bw when Sprague Dawlay male and female rats were treated with Diethylcarbamoyl chloride orally.

Executive summary:

In a acute oral toxicity study,Sprague Dawlay male and female rats were treated with Diethylcarbamoyl chloride undiluted in the concentration of2000, 2350, 2600 and 2900 mg/kg bw orally by gavage.All the five female rats were died at 2900 mg/kg bw. Half of deaths occurred in 30 minutes to two hours while the other occurred in 24 to 72 hours. 1 male and 1 female rat died at 2600 mg/kg bw, 1 male rat died at 2350 mg/kg bw and No mortality were observed in treated male and female rats at 2000 mg/kg bw. Muscular in coordination, lethargy, convulsions and coma were observed in died rats. Moderately severe diarrhea was observed in all surviving rats. Decreased in 8 to 15 % body weight was observed in treated rats. In addition, Alteration In Gastric Secretion, severally irritant intestinal mucosa, abnormally dark liver and greenish colure spleen was observed in treated rats. Hemorrhogic area in Liver and congestion of tubule in kidney were observed in treated rats. Therefore,LD50 was estimated to 2700 mg/kg bw whenSprague Dawlay male and female ratswere treated withDiethylcarbamoyl chloride orally by gavage.