1-chloro-2,5-dimethoxybenzene

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1977-01-05 to 1977-01-24

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well documented study report which meets basic scientific principles. Study was conducted prior to GLP and OECD guideline implementation.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1977

Report date:

1977

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: company guideline previous to OECD guideline implementation that is similar to OECD 402

Deviations:

not applicable

Principles of method if other than guideline:

Dermal toxicity was tested using 6 female SPF-Wistar-rats per dose. The rats were obtained from in-house breeding and had a mean body weight of 163g (ranging from 154-182g) before the experiment.
The liquid test substance was applied undilution in different doses directly onto the mechanically depilated intact skin. The treated skin area had a size of about 30cm2 and was covered with alumina foil (6x8cm) for 24hrs of exposure. The foil was fixated using elastic adhesive bandage (Elastoplast 8cm wide, Company Baiersdorf AG, Hamburg). After 24hrs application time the foil was removed and residues carefully washed away with a sponge and lukewarm water. All animals were observed at the day of application. During 14d post exposure observation period all surviving animals were weighed on a daily basis (except for the weekends) and their behaviour was observed. Animals were kept in single cages. Lethally intoxicated animals were dissected and macroscopically evaluated. At the end of the observation period surviving animals were narcotized and killed. After that they were dissected and macroscopically evaluated.

GLP compliance:

no

Remarks:

- Performed prior to GLP implementation

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

1-chloro-2,5-dimethoxybenzene

EC Number:

218-267-9

EC Name:

1-chloro-2,5-dimethoxybenzene

Cas Number:

2100-42-7

Molecular formula:

C8H9ClO2

IUPAC Name:

2-chloro-1,4-dimethoxybenzene

Details on test material:

- Name of test material (as cited in study report): CME = Chlorhydrochinondimethyläther (2-Chlor-1,4-dimethoxybenzol)

Test animals

Species:

rat

Strain:

Wistar

Sex:

female

Details on test animals or test system and environmental conditions:

- Single cages
- Food: Altromin 1324, ad libitum, Company: Altromina GmbH, Lage/Lippe
- Water: tap water, ad libitum

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

- Exposure 24hrs
- Different doses (1600, 2000, 4000, 5000 mg/kg bw)
- Skin was mechanically depilated, only non-injured areas were used
- Depilated area approx. 30cm2
- Applied substance was covered using alumina foil (6x8cm)
- Alumina foil was fixated using elastic adhesive bondage (Elastoplast)
- After exposure, residues of the substance were carefully removed using a sponge and warm water

Duration of exposure:

24hrs

Doses:

1600, 2000, 4000, 5000 mg/kg bw

No. of animals per sex per dose:

6 female SPF-Wistar-rats per dose

Control animals:

no

Statistics:

LD50 were calculated using Probit models (according to Linder and Weber), confidence intervals were calculated according to Cavalli-Sforza.

Results and discussion

Effect levels

Sex:

female

Dose descriptor:

LD50

Effect level:

2 194 mg/kg bw

95% CL:

1 169 - 4 119

Mortality:

1600 mg/kg bw: 3 out of 6 rats
2000 mg/kg bw: 2 out of 6 rats
4000 mg/kg bw: 4 out of 6 rats
5000 mg/kg bw: 5 out of 6 rats

Clinical signs:

other: Lethally intoxicated animals showed: - ataxic gate - squatting posture - prone position - decreased breathing rate - bad general state of health - narcosis-like condition

Gross pathology:

Pathology of the animals that died during the observation period:
- bright brown discoloured liver
- plethora of lungs

Pathology of surviving animals dissected after observational period:
- without pathological findings

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: expert judgment

Conclusions:

LD50: 2194mg/kg bw

Executive summary:

Testing for acute dermal toxicity of CME resulted in a LD50 of 2194mg/kg body weight.