Cyclohexane, oxidized, non-acidic by-products, distn. residues

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable well documented study report which meets basic scientific principles.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1962

Report date:

1962

Materials and methods

Principles of method if other than guideline:

BASF-Test: The substance was injected into the peritoneal cavity. Young adult laboratory mice were purchased from breeder. Usually the source and strain of animals were not documented. Several groups of 5 mice per sex and dose were treated simultaneously with preparations of the test substance in suitable vehicle. The concentrations of these preparations were used to achieve comparable volumes per kg body weight. Group-wise documentation of clinical signs was performed over the 7 day study period. Body weight was determined before the start of the study only, as it was needed for determination of dose.

GLP compliance:

no

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Cyclohexane, oxidized, non-acidic by-products, distn. residues

EC Number:

271-816-4

EC Name:

Cyclohexane, oxidized, non-acidic by-products, distn. residues

Cas Number:

68609-04-1

IUPAC Name:

(1s,4s)-cyclohexane-1,4-diol (cyclohexyloxy)cyclohexane 2-butylcyclohexan-1-one 8-oxatricyclo[7.4.0.0^{2,7}]trideca-1(9),2(7)-diene cyclohexane cyclohexane-1,3-diol cyclohexanol phenol hydrate

Details on test material:

- Name of test material (as cited in study report): Liquid Anon-destillation residue
- condensation products of cyclohexanol and cyclohexanon of unknown chemical structure. Ash-content approx. 2% (determined as sulfate-ash).

Test animals

Species:

mouse

Strain:

not specified

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Mean weight at study initiation:
2000 mg/kg bw: male 20-26 g, female 20-23 g
1600 mg/kg bw: male 26-35 g, female 16-23 g
1250 mg/kg bw: male 26-32 g, female 22-26 g
1000 mg/kg bw: male 23-30 g, female 18-26 g
800 mg/kg bw: male 27-33 g, female 23-27 g
640 mg/kg bw: male 22-30 g, female 21-25 g
500 mg/kg bw: male 22-33 g, female 16-32 g
400 mg/kg bw: male 22-27 g, female 21-23 g
200 mg/kg bw: female 17-23 g

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

other: traganth

Details on exposure:

VEHICLE
- Concentration in vehicle (%): 2, 4, 8 and 20

Doses:

2000, 1600, 1250, 1000, 800, 640, 500, 400 and 200 ml/kg bw (corresponding to 2103, 1682, 1314, 1052, 841, 673, 526, 421 and 210 mg/kg bw) Calculation based on the density of the test substance (1.0515 g/cm3 at 20 °C)

No. of animals per sex per dose:

5 for 400-2000 ml/kg bw dose groups,
5 (only females) for 200 ml/kg bw dose group

Control animals:

no

Details on study design:

- Duration of observation period following administration: 10 days
- Frequency of observations and weighing: mortality and clinical signs of toxicity were observed several times on the application day and thereafter once each working day, body weights were only recorded at the beginning of the study.
- Necropsy of survivors performed: yes

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LC50

Effect level:

ca. 1 682 mg/kg bw

Mortality:

see free text

Clinical signs:

agitation, trembling, apathy later atony, lateral position, narcosis state

Gross pathology:

Deceased animals: skicky organs in the abdominal cavity.
Sacrificed animals: substance rests on the serosa of abdominal organs

Any other information on results incl. tables

Dose (ml/kg bw)	Mortality in
	24 h	48 h	7 days	10 days
2000	5/10	8/10	9/10	10/10
1600	1/10	3/10	4/10	4/10
1250	0/10	0/10	3/10	3/10
1000	0/10	0/10	0/10	0/10
800	0/10	0/10	1/10	1/10
640	0/10	0/10	0/10	0/10
500	0/10	0/10	0/10	0/10
400	0/10	0/10	0/10	0/10
200	0/5	0/5	0/5	0/5

LD50 = 1600 ml/kg bw (corresponding to 1682 mg/kg bw)