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EC number: 252-961-2 | CAS number: 36306-87-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Qualifier:
- according to guideline
- Guideline:
- other: Commission Regulation (EC) No 440/2008 of 30 May 2008, Part C: Methods for the determination of ecotoxicity, Publication No. L142, C.1. "Acute Toxicity for Fish".
- Qualifier:
- according to guideline
- Guideline:
- other: Guidance document on aquatic toxicity testing of difficult substances and mixtures, OECD series on testing and assessment number 23, December 14, 2000.
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Identification: Kephalis
Appearance: Pale yellow liquid
Batch: PE00097828
Purity/Composition: 85.2% (sum of two peaks)
Test substance storage: In refrigerator (2-8°C) protected from light
Stable under storage conditions until: a14 March 2015 (expiry date)
Chemical name (IUPAC): 4-(1-ethoxyvinyl)-3,3,5,5,-tetramethylcyclohexanone (main component)
CAS Number: 36306-87-3
EC Number: 252-961-2
Molecular formula: C14H24O2
Molecular weight: 224.4 g/mol - Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- Kephalis: Solutions containing 4.6, 10, 22, 46 and 100% of the SS prepared at a loading rate of 100 mg/l.
Control: Test medium without test substance or other additives - Test organisms (species):
- Cyprinus carpio
- Details on test organisms:
- Species: Carp (Cyprinus carpio, Teleostei, Cyprinidae) Linnaeus, 1758
Source: Zodiac, proefacc, "De Haar Vissen", Wageningen University and Research Centre, The Netherlands.
Mean length:
Range-finding test: 2.1 ± 0.2 cm
Final test: 2.3 ± 0.1 cm
Mean weight:
Range-finding test: 0.24 ± 0.06 g
Final test: 0.31 ± 0.05 g
Characteristics: F1 from a single parent-pair bred in UV-treated water.
Reason for selection: This system has been selected as an internationally accepted species.
Total fish used: 54 - Test type:
- semi-static
- Water media type:
- freshwater
- Total exposure duration:
- 96 h
- Remarks on exposure duration:
- Semi-static, with one renewal of test solutions (48 hours)
- Hardness:
- Adjusted ISO medium with a hardness of 180 mg CaCO3 per litre
- Test temperature:
- 20-24°C
- pH:
- pH: 6.0-8.5
- Dissolved oxygen:
- oxygen > 60% of air saturation
- Nominal and measured concentrations:
- Nominal: 4.6, 10, 22, 46 and 100% of a saturated solution
Measured: 2.6, 5.9, 14, 198 and 53 mg/L - Reference substance (positive control):
- yes
- Remarks:
- pentachlorophenol (PCP); WIL Research Europe Project 508111 (Batch K 14-06)
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 5.9 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 9.1 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% confidence interval could not be determined
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 14 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Results with reference substance (positive control):
- The study procedure described in this report was based on the OECD guideline No. 203, 1992.
This reference test was carried out to check the sensitivity of the test system as used by WIL Research Europe. The reference substance was pentachlorophenol (PCP, SIGMA, Art. P2604, Batch MKBK1499V).
Concentrations: 0.10, 0.22 and 0.46 mg/l in ISO-medium.
During the test the pH, oxygen concentration and the temperature of the medium were within the optimal ranges for fish.
Under the conditions of the present test PCP induced no lethal effects in carp at 0.10 mg/l. The
96h-LC50 for carp exposed to PCP was 0.32 mg/l. This effect was already reached within 48 hours of exposure.
The range of the 96h-LC50 for carp is generally between 0.10 and 0.46 mg/l based on historical data of reference tests performed approximately every 3 months from April 1988 until the end of 2000, and annually since then. Hence, the sensitivity of carp originating from the present batch for PCP falls within the range of sensitivities generally observed during the past years.
The raw data and report from this study are kept in the WIL Research Europe archives. The test described above was performed under GLP-conditions with a QA-check. - Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of the present study the 96h-LC50 was 9.1 mg/l (95% confidence interval could not be determined).
Reference
Description of key information
The acute toxicity of the test item to Carp has been investigated and based on the mean measured test concentrations gave a 96-Hour LC50 value of 9.1 mg/l (95% confidence interval could not be determined). The study was performed according to GLP and OECD Guideline 203.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 9.1 mg/L
Additional information
Based on the results of a range-finding test, the definitive test was conducted at test concentrations of 4.6, 10, 22, 46 and 100% of a saturated solution prepared at a loading rate of 100 mg/L. The test was performed under semi-static conditions for 96 hours. Test solutions were renewed after 48 hours.
Samples for analysis of test concentrations were taken at the start and at the end of both refreshment periods or at the time when all fish in a given concentration were found dead. Because Kephalis was light sensitive and volatile, the exposure took place under dimmed light conditions and in airtight closed vessels with headspace reduced to minimum.Based on the analytical results, the average exposure concentrations were calculated to be 2.6, 5.9, 14, 19 and 53 mg/l in solutions corresponding respectively to the 4.6, 10, 22, 46 and 100% SS. The study met the acceptability criteria prescribed by the protocol and was considered valid.
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