Reaction mass of (E)-3,7-dimethylocta-2,6-dien-1-yl formate and 3,7-dimethyloct-6-en-1-yl formate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

April 1971

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

The study meets the EC Standards (conducted equivalent to OECD 401). Non GLP. Deviations: no details on test material, limited details on test animals and environmental conditions. However, dose, number of animals and observation period and considering mortality observed, the result is considered reliable with restrictions.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1971

Report date:

1971

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

GLP compliance:

no

Remarks:

study pre-dates introduction of GLP

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Geranyl formate

EC Number:

203-339-4

EC Name:

Geranyl formate

Cas Number:

105-86-2

Molecular formula:

C11H18O2

IUPAC Name:

(E)-3,7-dimethylocta-2,6-dien-1-yl formate

Constituent 2

Reference substance name:

Citronellyl formate

EC Number:

203-338-9

EC Name:

Citronellyl formate

Cas Number:

105-85-1

Molecular formula:

C11H20O2

IUPAC Name:

3,7-dimethyloct-6-en-1-yl formate

impurity 1

Reference substance name:

Geraniol

EC Number:

203-377-1

EC Name:

Geraniol

Cas Number:

106-24-1

Molecular formula:

C10H18O

IUPAC Name:

(E)-3,7-dimethylocta-2,6-dien-1-ol

impurity 2

Reference substance name:

Rhodinyl formate

EC Number:

205-456-6

EC Name:

Rhodinyl formate

Cas Number:

141-09-3

Molecular formula:

C11H20O2

IUPAC Name:

3,7-dimethyloct-7-en-1-yl formate

Test material form:

liquid

Specific details on test material used for the study:

- Name of test material (as cited in study report): CFI-70 Geranyl Formate
- Description: Colourless liquid
- Received: January 22, 1971

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 150 to 250 g
- Fasting period before study: overnight
- Housing: Individually housed
- Diet: Free access to commercial diets
- Water: Free access to water
- Acclimation period: No data

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

corn oil

Details on oral exposure:

VEHICLE
- Concentration in vehicle: 50%

MAXIMUM DOSE VOLUME APPLIED: 6 mL/kg

Doses:

6000 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations: Toxic signs and mortality were recorded at one and four hours and then once daily
- Necropsy of survivors performed: A necropsy was performed on all animals which died during the study and on those killed by cervical dislocation at termination.

Results and discussion

Preliminary study:

The results obtained from the range-finding study showed that no deaths occurred in rats which received CFI-70 at any dose level up to 10 mL/kg.

Effect levels

Sex:

not specified

Dose descriptor:

LD50

Effect level:

> 6 000 mg/kg bw

Based on:

test mat.

Mortality:

One animal died.

Clinical signs:

Piloerection and ataxia was observed in one animal.

Body weight:

No data.

Gross pathology:

The stomach of the animal that died 24 hours following administration of the test substance was filled with fluid. No tissue damage was seen in any animal killed at the termination of the study.

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

In an acute oral toxicity study with rats, performed equivalent to OECD 401 guidelines, an LD50 of >6000 mg/kg bw was determined.

Executive summary:

The test substance CFI-70 Geranyl Formate was tested in an acute oral toxicity study with rats, performed equivalent to OECD 401 guideline. One animal died. Piloerection and ataxia was observed in one animal. The stomach of the animal that died 24 hours following administration of the test substance was filled with fluid. No tissue damage was seen in any animal killed at the termination of the study.

Based on the results, an LD50 of >6000 mg/kg bw was determined.