Cuprate(6-), [2-[[[[3-[[4-chloro-6-[[4-[[4-chloro-6-[(3-sulfophenyl)amino]-1,3,5-triazin-2-yl]amino]-2,5-disulfophenyl]amino]-1,3,5-triazin-2-yl]amino]-2-hydroxy-5-sulfophenyl]azo]phenylmethyl]azo]-5-sulfobenzoato(8-)]-, pentasodium hydrogen, (SP-4-3)-

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

May 19, 2016 - June 24, 2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

GLP compliance:

no

Type of method:

flask method

Key result

Water solubility:

205 g/L

Conc. based on:

test mat.

Loading of aqueous phase:

802 g/L

Temp.:

20 °C

pH:

> 8.2 - < 8.5

  Preliminary visual estimation of the water solubility

In a stepwise procedure, increasing volumes of demineralized water were added at room temperature to a defined amount of the test item. After each addition of an amount of water the mixture was treated with ultrasound for 15 minutes and visually checked for any undissolved particles.

Table4: Results of the preliminary visual estimation of the water solubility

Amount of test item / mg		Added volume of dist. water / mL		Total volume / mL		Appearance of mixture
100.8		0.1		0.1		not dissolved
		0.4		0.5		dissolved

The preliminary visual experiments showed that the water solubility of the test item is between 202 g/L and 1008 g/L. According to the preliminary visual estimation in the main test the water solubility was determined by the flask method.

 

Prior to the performance of the main test, an additional stirring test was performed to determine the highest amount of test item that can still be stirred sufficiently in a mixture with water.

Table5: Stirring test

Amount of test item [g]		Added volume of dist. water [mL]		Total volume [mL]		Observation
5.525		5		5		could not be stirred at 20 °C
		1		6		could not be stirred at 20 °C
		1		7		could be stirred at 20 °C

According to the stirring test, mixtures of approx. 800 g/L test item in dist. water were used in the main test.

Flask method

The flask method was performed as described in chapter4.1.2. The data for the evaluation are given inTable6.The water solubility was calculated from the measured and unrounded concentration values and not from the rounded values given inTable6.

Table6: Water solubility experiments of the test item

Experiment	24 h experiment	48 h experiment	72 h experiment	Blank experiment (72 h)
Amount of test item / g	8.0212	8.0013	8.0427	--
Amount of demineralized water / mL	10	10	10	10
Stirring time at 30 °C / h	24	48	72	72
pH at 24 °C	8.5	8.4	8.2	5.8
Aliquot of the flasks / mL	0.25	0.25	0.25	0.25
1^stDilution to volume / mL	25	25	25	25
Aliquot of the flasks / mL	0.1	0.1	0.1	0.1
2^ndDilution to volume / mL	1	1	1	1
Dilution factor	1000	1000	1000	1000
Measured concentration / mg/L	240 / 238	183 / 183	192 / 194	-- / --
Mean concentration / mg/L	239	183	193	--
Water solubility / g/L	239	183	193	--

The water solubility of the test item at 20 °C was determined to be205 g/L (± 30 g/L).

Executive summary:

The water solubility c_Sof the test item Reactive Blue 160 (Cu formazan) at a temperature of 20 °C was determined according to the flask method. It was found to be:

                                                                 c_S=205 g/L (± 30 g/L)

Description of key information

The water solubility c_Sof the test itemReactive Blue 160 (Cu formazan)at a temperature of 20 °C was determined according to the flask method. It was found to be:

                                                                 c_S=205 g/L (± 30 g/L)

Key value for chemical safety assessment

Water solubility:

205 g/L

at the temperature of:

20 °C