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EC number: 269-522-6 | CAS number: 68259-31-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 8-isopropyl-6-methylbicyclo[2.2.2]oct-5-ene-2-carbaldehyde
- EC Number:
- 267-308-7
- EC Name:
- 8-isopropyl-6-methylbicyclo[2.2.2]oct-5-ene-2-carbaldehyde
- Cas Number:
- 67845-30-1
- Molecular formula:
- C13H20O
- IUPAC Name:
- 8-isopropyl-6-methylbicyclo[2.2.2]oct-5-ene-2-carbaldehyde
- Reference substance name:
- 7-isopropyl-5-methylbicyclo[2.2.2]oct-5-ene-2-carbaldehyde
- EC Number:
- 299-795-7
- EC Name:
- 7-isopropyl-5-methylbicyclo[2.2.2]oct-5-ene-2-carbaldehyde
- Cas Number:
- 93904-56-4
- Molecular formula:
- C13H20O
- IUPAC Name:
- 7-isopropyl-5-methylbicyclo[2.2.2]oct-5-ene-2-carbaldehyde
- Reference substance name:
- rel-(1S,2R,4R)-1-isopropyl-4-methylbicyclo[2.2.2]oct-5-ene-2-carbaldehyde
- Cas Number:
- 36208-34-1
- Molecular formula:
- C13H20O
- IUPAC Name:
- rel-(1S,2R,4R)-1-isopropyl-4-methylbicyclo[2.2.2]oct-5-ene-2-carbaldehyde
- Test material form:
- liquid
Constituent 1
Constituent 2
impurity 1
- Specific details on test material used for the study:
- Test substance name: Maceal
Chemical name: 5(or 6)-methyl-7(or 8)-(1-methylethyl)-bicyclo[2.2.2]oct-5-ene-2-carboxaldehyde
CAS number: 68259-31-4
Molecular formula: C13H20O
Molecular weight: 192 g/mol
Water solubility: 27.11 mg/L at 20 ºC
Vapour pressure: 1.647 Pa at 20 ºC 2.448 Pa at 25 ºC
Octanol water partition co-efficient (Log Pow): 3.98 to 5.41 at 25 ºC
Batch number: SC00008034
Description: Clear to Pale Yellow Liquid
Purity: 99.2%
Storage: Refrigerated (2 – 8°C), in the dark
Expiry date: 16 October 2015
Date received: 02 June 2014
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- At approximately 24-hour intervals after the start of the incubation period, pre-determined volumes of test media were removed from each incubated test vessel, and transferred to individually identified cell counting vials. The contents of each vial were diluted to a 10 mL final volume with an electrolyte solution. The cell density of the vial contents was then determined using a particle counter.
Test solutions
- Vehicle:
- yes
Test organisms
- Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- The test organism, Pseudokirchneriella subcapitata (Strain 278/4), was originally obtained from the Culture Collection of Algae and Protozoa (CCAP) and is a representative species of the freshwater aquatic phytoplankton. This species is recommended for testing in accordance with the OECD regulatory guidelines.
Prior to testing, duplicate starter cultures were prepared and incubated under test conditions to obtain sufficient algal cells in exponential growth and to achieve a starting algae cell density of 1 x 104 cells/mL.
Positive control tests are conducted periodically to assess the performance of the test species.
Study design
- Test type:
- not specified
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- The laboratory temperature was set within the range 21 to 24°C and maintained within ± 2°C for the duration of the test. The temperature was recorded continuously using a digital temperature logger.
- pH:
- At the start of the test, the pH of freshly prepared test media, before and after alga cell inoculation was determined. The pH in each test vessel was also determined at the end of the test.
- Nominal and measured concentrations:
- The definitive test was conducted at nominal test concentrations of 1.0, 3.2, 10, 32 and 100% saturated solution (0.0729, 0.140, 0.311, 0.426 and 1.449 mg/L based on geometric mean measured concentrations).
- Details on test conditions:
- Orbital Shaker Speed: 100 rpm
Light Intensity (Lux): 6660 - 6670 - Reference substance (positive control):
- yes
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.912 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Remarks on result:
- other: 95% confidence limits: 0.626-1.147 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.877 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: 95% confidence limits: 0.660 - 1.116 mg/L
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1.449 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.616 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% confidence limits: 0.468-0.935
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.426 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield, biomass and growth rate
- Details on results:
- Based on geometric mean measured test concentrations, the 72-hour EyC50 and the 0-72 hour EbC50 and ErC50 values were calculated to be 0.912, 0.877 and greater than 1.449 mg/L, respectively. The 72-hour EyC10 and the 0-72 hour EbC10 and ErC10 values were calculated to be 0.378, 0.327 and 0.616 mg/L, respectively. The corresponding NOEC values for yield, biomass and specific growth rate after 72 hours were 0.426 mg/L.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The effects of Maceal on the growth of the unicellular green alga, Pseudokirchneriella subcapitata, were determined during a 72-hour growth inhibition toxicity test conducted in accordance with OECD Chemicals Testing Guideline No. 201 Alga, Growth Inhibition Test (adopted 23 March 2006) (Annex 5 corrected 28 July 2011).
Based on geometric mean measured test concentrations, the 72-hour EyC50 and the 0-72 hour EbC50 and ErC50 values were calculated to be 0.912, 0.877 and greater than 1.449 mg/L, respectively. The 72-hour EyC10 and the 0-72 hour EbC10 and ErC10 values were calculated to be 0.378, 0.327 and 0.616 mg/L, respectively.The corresponding NOEC values for yield, biomass and specific growth rate after 72 hours were 0.426 mg/L.
The preferred observational end point in the algal growth inhibition test is growth rate because it is not dependent on the test design (ECHA guidance Chapter R.7b v1.1). The EU CLP regulation (No 1272/2008 and its adaption 286/2011) also states that classification should be based on the ErC50. The preferred observational endpoint in long-term studies is the EC10 value because it is derived from the dose response curve. In contrast the NOEC strongly depends on the experiment design (e.g. the concentrations used in the test). Thus the 72-h EC50 and EC10 based on growth rate are used for classification purposes, which were determined in this study to be > 1.499 mg/L and 0.616 mg/L respectively.
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