Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 604-351-6 | CAS number: 143390-89-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to fish
Administrative data
- Endpoint:
- adult fish: sub(lethal) effects
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study with analytical monitoring
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study)
- Deviations:
- yes
- Remarks:
- , exposure period: 28 days
- GLP compliance:
- yes
Test material
- Reference substance name:
- methyl (2E)-2-methoxyimino-2-[2-[(2-methylphenoxy)methyl]phenyl]acetate
- EC Number:
- 604-351-6
- Cas Number:
- 143390-89-0
- Molecular formula:
- C18 H19 N O4
- IUPAC Name:
- methyl (2E)-2-methoxyimino-2-[2-[(2-methylphenoxy)methyl]phenyl]acetate
- Details on test material:
- - Name of test material (as cited in study report): Reg.No. 242 009
- Physical state: soild (powder)
- Analytical purity: 94.3% active ingredient
- Batch No.: N 36
- Storage condition of test material: room temperature
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling time: at Day 0, 2, 7, 14, 21, 28
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: One stock solution, containing 1.0 mg/L test compound (nominal), was prepared for all test concentrations by dissolving the respective quantity of the test compound in drinking water. To improve the stability of the test compound in water the pH of the stock solution was adjusted to pH 5 with sulforic acid (approx. 20%). To improve the dilution of the test substance in the test water 1 mg test component was predissolved in 1 ml methanol. This solution in methanol was introduced into the respective quantity of test water while being stirred vigorously.
Aliquot amounts of this stock solution containing 1 mg/L test water were transferred into separate vessels, one for each concentration, and then different quantities of methanol were added to obtain the same methanol content in all test solutions.
- Chemical name of vehicle: Methanol
Test organisms
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: rainbow trout
- Source: Forellenhof Fredelsloh, Trout Breeding, Moring 1, Germany
- Hatched: Nov 1993
- Length at study initiation (mean, range): 5.6 cm, 5.0 – 6.2 cm
- Weight at study initiation (mean, range): 1.5 g, 1.2 – 1.9 g
- Feeding during test: yes
- Food type: Ssniff trout starter FB-50/E size 1, of Ssniff, 59494 Soest, Germany
- Amount, frequency: 4% of the mean body weights on working days daily in 2 portions and on non-working days in one portion. Additionally on working days frozen artemia
ACCLIMATION
- Acclimation period: 2.5 months
- Acclimation conditions: same as test
- Type of food: Ssniff trout starter FB-50/E size 1, of Ssniff, 59494 Soest, Germany
- Amount, feeding frequency: About 1 to 2% of their mean body weight daily in 2 portions on workdays and in one portion on non-working days; additionally they received on workdays frozen artemia.
- Health during acclimation: After arrival in the laboratory the fish were treated prophylactically twice with 0.05 mg/L malachite green chloride and once with 10 mg/L tetracycline hydrochloride for about 24 hours each.
- Mortality during final adaption (ca. 1 week): 0.8%
Study design
- Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 28 d
Test conditions
- Hardness:
- 2.3 mmol/L
- Test temperature:
- 14 ± 1 °C
- pH:
- 8.1 - 8.3
- Dissolved oxygen:
- 9 -10 mg/L
- Nominal and measured concentrations:
- Nominal test concentrations: control, 0.004, 0.02, 0.1, 0.5 mg/L
Recovery rate: n.d., -, 79.6, 66.4, 91.3%
n.d. = non-detectable - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, fill volume: glass aquaria in a stainless steel frame, 60 x 35 x 40 cm, 60 L
- Aeration: continuously aerated with oil-free air
- Renewal rate of test solution (flow rate): 10 L/hour/test aquarium
- No. of organisms per vessel: 20
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: drinking water purified over active carbon and aerated
- Culture medium different from test medium: no
- Intervals of water quality measurement: temperature daily, pH and oxygen content twice weekly
OTHER TEST CONDITIONS
- Photoperiod: 16 hours light : 8 hours darkness
EFFECT PARAMETERS MEASURED: mortality daily; toxic signs on each working day; body length and weight were determined at the beginning and at the end of the study
TEST CONCENTRATIONS
- Range finding study: preceding invalid 28-day study was used for selecting the concentrations
- Test concentrations and mortality: at 0.05 and 1.0 mg/L 1/20 fish died. At 0.01 and 0.001 mg/L no mortality occurred. Toxic symptoms were observed in the concentrations 0.05 and 0.1 mg/L for only one day
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.013 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.013 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- act. ingr.
- Basis for effect:
- behaviour
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.013 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality
- Details on results:
- BODY WEIGHT
- At the end of the study (day 28) no significant differences in the mean body weight between the negative control (5.0 g), solvent control (5.1 g) and the treatment groups 0.004 and 0.02 mg/L (5.0 and 4.8 g) are observed. In the 0.1 mg/L group the mean weight was only 3.1 g. No body weight could be measured in the 0.5 mg/L group since there were no survivors.
- Threshold level for the impairment of the growth parameter body weight was greater than 0.02 mg/L and smaller than 0.1 mg/L.
BODY LENGTH
- At the end of the study (day 28) no significant differences in the mean body length between the negative control (7.7 cm), solvent control (7.7 cm) and the treatment groups 0.004 and 0.02 mg/L (7.6 cm) are observed. In the 0.1 mg/L group the mean weight was only 6.7 cm. No body length could be measured in the 0.5 mg/L group since there were no survivors.
- Threshold level for the impairment of the growth parameter body length was greater than 0.02 mg/L and smaller than 0.1 mg/L.
TOXIC SIGNS
- Compound-related toxic signs were observed in the concentration group 0.1 mg/L in the form of apathy, reduced or no uptake of food and discoloration (dark). Symptoms commenced on day 15 and lasted through day 28 of the study (as single occurrences or combined). No toxic signs were observed in all the other test groups containing test compound and in the controls.
- The threshold level for toxic signs (= symptoms) was greater than 0.02 mg/L and smaller than 0.1 mg/L.
MORTALITY
- Mortalities occurred in the two highest test groups: In the 0.1 mg/L group, starting at day 18 (20%) and with 35% dead fish at day 28 the end of the study. In the 0.5 mg/L group all fish died already on day zero of the study. In the control groups as well as in the lower concentrations (0.004 and 0.02 mg/L) no mortalities occurred. The lowest concentration at which mortalities occurred was 0.1 mg/L.
- The threshold level of lethal effects was greater than 0.02 and smaller than 0.1 mg/L.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.