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EC number: 228-711-3 | CAS number: 6334-18-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 429 (Skin Sensitisation: Local Lymph Node Assay)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of study:
- mouse local lymph node assay (LLNA)
Test material
- Reference substance name:
- 2,3-dichlorobenzaldehyde
- EC Number:
- 228-711-3
- EC Name:
- 2,3-dichlorobenzaldehyde
- Cas Number:
- 6334-18-5
- Molecular formula:
- C7H4Cl2O
- IUPAC Name:
- 2,3-dichlorobenzaldehyde
- Test material form:
- solid: crystalline
- Details on test material:
- Test item: 2,3-dichlorobenzaldehydeCas No.: 6334-18-5Storage: 15-30 °C
Constituent 1
In vivo test system
Test animals
- Species:
- mouse
- Strain:
- CBA/Ca
- Sex:
- female
- Details on test animals and environmental conditions:
- Species and strain: CBA/Ca Ola Hsd miceSource: TOXI-COOP ZRT.Hygienic level at arrival: SPFHygienic level during the study: Good conventional.Number of animals: 28 animals/main test (4 animals/treatment group)Sex: Female, nulliparous, non-pregnantAge of animals: Young adult mice; 10-11 weeks old (at start of the main test)Body weight range at starting: 19.0-23.0 g The weight variation in animals involved in the study did not exceed ± 20 % of the mean weight.Acclimatization time: 7 daysAnimal health: Only healthy animals were usedHousing during acclimatization period: Grouped caging in small groupsHousing during the test: Grouped caging (4 animals/cage)Light: 12 hours daily, from 6.00 a.m. to 6.00 p.m.Temperature: 22 ± 3 °CRelative humidity: 30 – 70 %
Study design: in vivo (LLNA)
- Vehicle:
- dimethylformamide
- Concentration:
- Test item concentration: 0.5; 0.25; 0.1 ; 0.05 % w/v
- No. of animals per dose:
- 4 animals/treatment group
- Details on study design:
- Animals in the treatment groups were treated with the relevant vehicles (DMF or AOO), appropriate formulations of the test item or 25 % concentration of the positive control substance. The test item was administered at four different concentrations according to the results of the dose range finding test.
- Positive control substance(s):
- hexyl cinnamic aldehyde (CAS No 101-86-0)
Results and discussion
- Positive control results:
- The positive control group animals were treated with 25 % (w/v) HCA solution (dissolved in AOO) concurrent to the test item groups. No mortality, cutaneous reactions or signs of toxicity were observed in the positive control group.Significant lymphoproliferative response (SI ≥ 3) was noted for HCA (SI = 5.0). The results of the positive control item demonstrated appropriate performance of the test in accordance with the relevant guidelines and confirmed validity of the assay.
In vivo (LLNA)
Resultsopen allclose all
- Parameter:
- EC3
- Value:
- 0.09
- Remarks on result:
- other: based on the dose response curve
- Parameter:
- EC3
- Value:
- 0.11
- Remarks on result:
- other: based on the regression curve
- Parameter:
- SI
- Value:
- 3.9
- Remarks on result:
- other: 2,3 -dichloro-benzaldehyde 0.5% in DMF
- Parameter:
- SI
- Value:
- 3.6
- Remarks on result:
- other: 2,3-dichloro-benzaldehyde 0.25 % in DMF
- Parameter:
- SI
- Value:
- 3.1
- Remarks on result:
- other: 2,3-dichloro-benzaldehyde 0.1 % in DMF
- Parameter:
- SI
- Value:
- 2.6
- Remarks on result:
- other: 2,3-dichloro-benzaldehyde 0.05% in DMF
Any other information on results incl. tables
Test GroupName |
Measured DPM/group |
Group* DPM |
DPN (DPM/Node) |
Stimulation IndexValues |
Vehicle control for the positive control: AOO |
23412 |
23388.5 |
2923.6 |
1.0 |
Positive control: |
117447 |
117423.5 |
14677.9 |
5.0 |
2,3 -dichloro-benzaldehyde |
16534 |
16510.5 |
2063.8 |
3.9 |
2,3-dichloro-benzaldehyde 0.25 % in DMF |
15018 |
14994.5 |
1874.3 |
3.6 |
2,3-dichloro-benzaldehyde |
12904 |
12880.5 |
1610.1 |
3.1 |
2,3-dichloro-benzaldehyde |
10880 |
10856.5 |
1357.1 |
2.6 |
Vehiclecontrol for the test item: DMF |
4231 |
4207.5 |
525.9 |
1.0 |
*Group DPM = measured DPMgroup- average DPMbackground
Applicant's summary and conclusion
- Interpretation of results:
- Category 1A (indication of significant skin sensitising potential) based on GHS criteria
- Conclusions:
- Under the conditions of the present Local Lymph Node Assay, 2,3-DICHLORO-BENZALDEHYDE (CAS No. 6334-18-5) tested at the maximum feasible (non-toxic, non-irritant) concentration of 0.5 % and at concentrations of 0.25 %, 0.1 % and 0.05 % (w/v) as formulations in an appropriate vehicle (DMF) was shown to have skin sensitization potential.Based on the EC3 values calculated using dose-response and regression curve analysis the test item was considered as strong/extreme skin sensitizer.
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