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EC number: 700-316-5 | CAS number: 1155405-88-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- on day 1998-08-27
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 998
- Report date:
- 1998
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Tri (hexyl, octyl, decyl) citrate
- IUPAC Name:
- Tri (hexyl, octyl, decyl) citrate
- Reference substance name:
- -
- EC Number:
- 430-290-8
- EC Name:
- -
- IUPAC Name:
- 430-290-8
- Reference substance name:
- 2-Hydroxypropane-1,2,3-tricarboxylic acid, tri (hexyl, octyl, decyl) ester
- IUPAC Name:
- 2-Hydroxypropane-1,2,3-tricarboxylic acid, tri (hexyl, octyl, decyl) ester
- Details on test material:
- - Name of test material (as cited in study report): Tri (hexyl, octyl, decyl) citrate
- Substance type: pure active substance
- Physical state: liquid
- Stability under test conditions: in water for at least 2 hours
- Storage condition of test material: in original container, at room temperature, in the dark
Constituent 1
Constituent 2
Constituent 3
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Melting point: -29°C (freezing point: -35.5°C)
- Boiling point: test substance showed decomposition above 260°C
- Vapour pressure: 8.5 x 10E-3 p at 25°C
- Water solubility (under test conditions): < 1 mg/L
- Solubility in organic solvents: soluble
- log Pow: > 6
- pKa: not applicable
- Base or acid catalysis of test material: no data
- UV absorption: no data
- Stability of test material at room temperature: stable
- pH dependance on stability: no data
OTHER PROPERTIES (if relevant for this endpoint)
- Adsorption characteristics: no data
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The test substance was directly weighed into the test flasks. Then, the test flasks were filled each with 284 mL tap water.
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Laboratory culture: no
- Method of cultivation: not applicable
- Preparation of inoculum for exposure: The sludge was washed by centrifugation and the supernatant liquid phase was decanted. The solid material was resuspended in tap water and again centrifuged. The latter procedure was repeated twice. An aliquot of the final sludge suspension was weighed, dried and the ratio of wet sludge to its dry weight determined.
- Pretreatment: Calculated aliquots of washed sludge suspension, corresponding to 3 g dry material/L were made up with tap water. To this mixture, 50 mL synthetic sewage feed/L was added daily, starting 3 days prior to use, and the sludge was kept at room temperaure under continuous aeration until use. Just before the start of the test the dry weight was again measured and adjusted to 2 g dry weight/L.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Post exposure observation period:
- none
Test conditions
- Hardness:
- not mentioned
- Test temperature:
- 19 °C
- pH:
- 7.6 - 8.3
- Dissolved oxygen:
- 5.4 - 9.2 mg/L
- Nominal and measured concentrations:
- Nominal: 10, 32, 100, 320, 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 1 L glass flask with a fill volume of 500 mL
- Aeration: compressed air at an air-flow rate of approx. 0.6 L/min
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water, synthetic sewage feed according to guideline
OTHER TEST CONDITIONS
- Adjustment of pH: no
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Respiration rate of each test medium after 3 hours was measured with an oxygen electrode (ECM-multi, Dr. Lange, Germany), and was recorded for 10 minutes. During measurement, the samples were continuously stirred on a magnetic stirrer. The pH amd the oxygen concentrations were determined before the start of the test and at the end of the incubation period in all test concentrations and controls. The water temperature was measured in one control medium at the start and the end of the incubation period.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: not mentioned
- Range finding study. Not performed - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol (final concentrations in the test: 10, 32 and 100 mg/L)
Results and discussion
Effect concentrations
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- - Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: yes - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: 3-hour EC50 = 15 mg/L - Reported statistics and error estimates:
- no statistics performed
Any other information on results incl. tables
Table: Influence of test substance and reference substance on oxygen consumption of activated sludge
Treatment | Concentration [mg/L] | Oxygen consumption[mg O2/L min] | Inhibition[%] |
Control | - | 0.411 / 0.379* | - |
Test substance | 10 | 0.337 | 14.8 |
Test substance | 32 | 0.362 | 8.3 |
Test substance | 100 | 0.378 | 4.4 |
Test substance | 320 | 0.316 | 20.1 |
Test substance | 1000 | 0.349 | 11.7 |
3,5 -DCP** | 10 | 0.288 | 27.1 |
3,5 -DCP** | 32 | 0.036 | 90.9 |
3,5 -DCP** | 100 | 0.039 | 90.1 |
* repeated test ** reference substance 3,5 -Dichlorophenol
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- The respiration rates of the two control did not differ by more than 15%. The 3-hour EC50 fo the reference substance was in the range of 5 to 30 mg/L. The concentration of dissolved ocygen did not drop below 2.5 mg/L during the incubation period.
- Conclusions:
- In comparison to the inoculum controls the respiration rate of the activated sludge was inhibited between 4.4 % and 20.1 % up to the highest tested concentration of nominal 1000 mg test substance/L. However, no concentration-effect relationship could be determined. Thus, the respiration rate was not significantly inhibited up to the highest tested concentration. Concentrations exceeding 1000 mg/L nominal were not tested.
From these inhibition rates, the 3-hour EC20 and EC50 could not be quantified. Nevertheless, the 3-hour EC50 was clearly higher than 1000 mg test substance/L under the present test conditions.
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