Hydrogen [4-[4-(diethylamino)-2',4'-disulphonatobenzhydrylidene]cyclohexa-2,5-dien-1-ylidene]diethylammonium, sodium salt

Short-term toxicity to fish

Description of key information

Fish Acute Toxicity test according to OECD Guideline 203 was conducted forHydrogen [4-[4-(diethylamino)-2', 4'-disulphonatobenzhydrylidene] cyclohexa-2, 5-dien-1-ylidene] diethyl ammonium, sodium salt. The nominal concentration selected for the experiment were 6.25 mg/L, 12.5mg/L,25mg/L, 50mg/L, 100mg/L and Zebra Fish (Danio rerio)were exposed to these concentration for 96 hours. The lethal concentrations LC50 was found to be > 100mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L

Additional information

Short term toxicity to fish was assessed for the target compound Hydrogen [4-[4-(diethylamino)-2', 4'-disulphonatobenzhydrylidene] cyclohexa-2, 5-dien-1-ylidene] diethylammonium, sodium salt (CAS no. 129-17-9) and its related read- across by reviewing a number of studies. The summary of the results are presented below:

In key study from UERL laboratory report; 2016 indicate that the Fish Acute Toxicity test conduct according to OECD Guideline 203 was conducted for Hydrogen [4-[4-(diethylamino)-2', 4'-disulphonatobenzhydrylidene] cyclohexa-2, 5-dien-1-ylidene] diethyl ammonium, sodium salt. The nominal concentration selected for the experiment were 6.25 mg/L, 12.5mg/L,25mg/L, 50mg/L, 100mg/L and Zebra Fish (Danio rerio)were exposed to these concentration for 96 hours. The lethal concentrations LC50 was found to be > 100mg/L.

Whereas predicted study (from ECOSAR version 1.10 predicted model from EPI suite) for target chemical (Cas no. 129-17-9) indicate that the lethal concentrations i.e LC50 of test substance for fish was predicted to be 4480000 mg/l in freshwater and 5530000 mg/l in salt water for 96 hrs.

And other experimental study (The journal of Toxicological sciences,Vol.7.193-203;1982) for read across Acid Violet 6B (Cas no. 1694-09-3)indicate that the Fish toxicity study was conducted for 24 hrs and 48 hrs for evaluating the lethal concentration of test substance Acid Violet 6B on fish Oryzias latipes on the basis of mortality effect.

During experiment the Median Tolerance Limit i.e lethal concentration (LC50) for test substance Acid Violet 6B on fish Oryzias latipes was found to be 950 mg/l for 24 hrs. and 760 mg/l for 48 hrs.

From same experimental source for another read across substance Light green SF (Cas no. 5141-20-8) indicate that the lethal concentration i.e LC50 on fish Oryzias latipes was found to be 1000 mg/l for 24 hrs. and 48 hrs.

And for read across substance Guinea green B (Cas no. 4680-78-8) indicate that the lethal concentration i.e LC50 on fish Oryzias latipes was found to be 1000 mg/l for 24 hrs. and 990 mg/l for 48 hrs.

From authorative database ECOTOX the lethal concentration for read across N-Ethyl-N-[4[[4-[ethyl[(3-sulfophenyl)methyl]amino]phenyl](2-sulfophenyl)methylene]-2,5-cyclohexadien-1-ylidene]-3-sulfobenzenemethanaminium hydroxide inner salt disodium salt (Cas no. 3844-45-9)on fish was found to be Oncorhynchus gorbuscha (Pink Salmon) was found to be 398 mg/l for 96 hrs.

Based on above available studies for target chemical and its read across it is concluded that the substance Hydrogen [4-[4-(diethylamino)-2', 4'-disulphonatobenzhydrylidene] cyclohexa-2, 5-dien-1-ylidene] diethylammonium, sodium salt (CAS no. 129-17-9) not qualify for the aquatic classification as per the CLP criteria.