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EC number: 224-924-0 | CAS number: 4553-89-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Acute
toxicity: oral
The
median lethal dose (LD50) for the substance Disodium
4,4'-[[2,4-dihydroxy-5-(hydroxymethyl)-1,3-phenylene]bis(azo)]bisnaphthalene-1-sulphonate
is determined to be > 2000 mg/Kg in female Evans albino mice.
Acute
toxicity: inhalation
In
accordance with column 2 of Annex VIII of the REACH regulation, testing
by the inhalation route is appropriate if exposure of humans via
inhalation is likely taking into account the vapour pressure of the
substance. This chemical has a very low vapour pressure of 1.48E-33
Pascal at 25°C and thus exposure by the inhalation route for this
chemical is unlikely.
Acute
toxicity: dermal
The
median lethal dose (LD50) for the substance Disodium
4,4'-[[2,4-dihydroxy-5-(hydroxymethyl)-1,3-phenylene]bis(azo)]bisnaphthalene-1-sulphonate
is estimated to be 35636.23 mg/Kg in rabbits via dermal route.
Key value for chemical safety assessment
Acute toxicity: via oral route
Link to relevant study records
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data is from a peer reviewed publication
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- Aqueous solutions of Chocolate Brown HT in a dose volume of 10 ml/kg were administered to groups of 10 mice by oral intubation. Survivors were observed and number of deaths noted for 14 days.
- GLP compliance:
- not specified
- Test type:
- other: No data available
- Species:
- mouse
- Strain:
- other: Evans Albino
- Sex:
- female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: No data available
- Age at study initiation: No data available
- Weight at study initiation: 16-20 g
- Fasting period before study: 18 hrs
- Housing: No data available
- Diet (e.g. ad libitum): No data available
- Water (e.g. ad libitum): No data available
- Acclimation period: No data available
ENVIRONMENTAL CONDITIONS
- Temperature (°C): No data available
- Humidity (%):No data available
- Air changes (per hr): No data available
- Photoperiod (hrs dark / hrs light): No data available
IN-LIFE DATES: From: To: No data available - Route of administration:
- oral: gavage
- Vehicle:
- water
- Details on oral exposure:
- Aqueous solutions of Chocolate Brown HT in a dose volume of 10 ml/kg were administered.
- Doses:
- upto 2000 mg/kg
- No. of animals per sex per dose:
- 10
- Control animals:
- not specified
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: No data available
- Necropsy of survivors performed: yes/no
- Other examinations performed: mortality, clinical signs, urine analysis
other: - Statistics:
- The acute LD50 value was calculated by the method of Litchfield & Wilcoxon (1949)
- Key result
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- No death observed during the observation period
- Clinical signs:
- other: Brown coloration especially of the extremities was observed
- Gross pathology:
- No data available
- Other findings:
- Elimination of orally-administered Chocolate Brown HT proceeded slowly, as evidenced by the persistence of brown colouring in the urine after a single dose
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- The median lethal dose (LD50) for the substance Disodium 4,4'-[[2,4-dihydroxy-5-(hydroxymethyl)-1,3-phenylene]bis(azo)]bisnaphthalene-1-sulphonate is determined to be > 2000 mg/Kg in female Evans albino mice.
- Executive summary:
Acute toxicity study was conducted on 10 female Evans albino mice for the test material Chocolate Brown HT ( Disodium 4,4'-[[2,4-dihydroxy-5-(hydroxymethyl)-1,3-phenylene]bis(azo)]bisnaphthalene-1-sulphonate). Aqueous solution of the test material was administered at a dose volume of 10mL/Kg to the mice by oral intubation. On the basis of mortality, clinical signs observed and urinalysis performed, the LD50 for the given test material is determined to be >2000 mg/Kg. This value indicates that the substance is non-toxic by oral route as per the CLP criteria.
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed
- Dose descriptor:
- LD50
- Value:
- 2 000 mg/kg bw
- Quality of whole database:
- Data is of K2 level from a peer reviewed publication
Acute toxicity: via inhalation route
Link to relevant study records
- Endpoint:
- acute toxicity: inhalation
- Data waiving:
- other justification
- Justification for data waiving:
- the study does not need to be conducted because exposure of humans via inhalation is not likely taking into account the vapour pressure of the substance and/or the possibility of exposure to aerosols, particles or droplets of an inhalable size
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed
Acute toxicity: via dermal route
Link to relevant study records
- Endpoint:
- acute toxicity: dermal
- Type of information:
- (Q)SAR
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- results derived from a valid (Q)SAR model and falling into its applicability domain, with limited documentation / justification
- Justification for type of information:
- The prediction is done using QSAR Toolbox version 3.3 and thr supportig QMRF has been attached.
- Principles of method if other than guideline:
- The prediction is done using QSAR Toolbox version 3.
- GLP compliance:
- no
- Test type:
- standard acute method
- Species:
- rabbit
- Strain:
- not specified
- Sex:
- not specified
- Type of coverage:
- not specified
- Vehicle:
- not specified
- Duration of exposure:
- 24 hr
- Doses:
- no data available
- No. of animals per sex per dose:
- no data available
- Control animals:
- not specified
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 35 636.23 mg/kg bw
- Based on:
- test mat.
- Mortality:
- no data available
- Clinical signs:
- other: no data available
- Gross pathology:
- no data available
- Other findings:
- no data available
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- The median lethal dose (LD50) for the substance Disodium 4,4'-[[2,4-dihydroxy-5-(hydroxymethyl)-1,3-phenylene]bis(azo)]bisnaphthalene-1-sulphonate is estimated to be 35636.23 mg/Kg in rabbits via dermal route.
- Executive summary:
The acute toxicity by dermal route of Disodium 4,4’-(2,4-dihydroxy-5- hydroxymethyl-1,3-phenylene bis-azo) di- (naphthalene-1-sulfonate) is estimated using QSAR Toolbox version 3.3. The median lethal dose (LD50) for the substance Disodium 4,4'-[[2,4-dihydroxy-5-(hydroxymethyl)-1,3-phenylene]bis(azo)]bisnaphthalene-1-sulphonate is estimated to be 35636.23 mg/Kg in rabbits via dermal route. This value indicates that the substance is not likely to exhibit toxicity by dermal route as per the CLP criteria.
Reference
The
prediction was based on dataset comprised from the following
descriptors: LD50
Estimation method: Takes average value from the 5 nearest neighbours
Domain logical expression:Result: In Domain
(((("a"
or "b" or "c" or "d" or "e" )
and ("f"
and (
not "g")
)
)
and ("h"
and (
not "i")
)
)
and ("j"
and "k" )
)
Domain
logical expression index: "a"
Referential
boundary: The
target chemical should be classified as Naphthalene sulfonic acids,
condensates by OECD HPV Chemical Categories
Domain
logical expression index: "b"
Referential
boundary: The
target chemical should be classified as No alert found by DNA binding by
OECD
Domain
logical expression index: "c"
Referential
boundary: The
target chemical should be classified as Non binder, MW>500 by Estrogen
Receptor Binding
Domain
logical expression index: "d"
Referential
boundary: The
target chemical should be classified as No alert found by Protein
binding by OECD
Domain
logical expression index: "e"
Referential
boundary: The
target chemical should be classified as Acid moiety AND Benzyl Alcohols
AND Phenols, Poly AND Salt by Aquatic toxicity classification by ECOSAR
Domain
logical expression index: "f"
Referential
boundary: The
target chemical should be classified as Alcohol AND Anion AND Aromatic
compound AND Azo compound AND Cation AND Hydroxy compound AND Phenol AND
Primary alcohol AND Sulfonic acid derivative by Organic functional
groups, Norbert Haider (checkmol)
Domain
logical expression index: "g"
Referential
boundary: The
target chemical should be classified as 1,2-aminoalcohol OR 1,2-diol OR
Acetal OR Aldehyde OR Alkene OR Alkyl bromide OR Alkyl chloride OR Alkyl
fluoride OR Alkyl halide OR Alkylarylether OR Alkylthiol OR Alkyne OR
Alpha-hydroxyacid OR Amine OR Aryl bromide OR Aryl chloride OR Aryl
fluoride OR Aryl halide OR Carbamic acid ester (uretane) OR Carbonic
acid derivative OR Carbonic acid diester OR Carbonic acid ester halide
OR Carbonyl compound OR Carboxylic acid OR Carboxylic acid amide OR
Carboxylic acid anhydride OR Carboxylic acid derivative OR Carboxylic
acid ester OR Carboxylic acid orthoester OR Carboxylic acid prim. amide
OR Carboxylic acid salt OR Carboxylic acid sec. amide OR Carboxylic acid
tert. amide OR CO2 derivative (general) OR Dialkylether OR Diarylether
OR Disulfide OR Enolether OR Ether OR Guanidine OR Halogen derivative OR
Hemiacetal OR Heterocyclic compound OR Hydroperoxide OR Hydroxylamine OR
Imine OR Isothiourea OR Ketone OR Lactam OR Lactone OR Nitrate OR
Nitrile OR Nitro compound OR No functional group found OR N-oxide OR
Orthocarboxylic acid derivative OR Oxime OR Peroxide OR Phosphine OR
Phosphinoxide OR Phosphonic acid OR Phosphonic acid derivative OR
Phosphonic acid ester OR Phosphoric acid OR Phosphoric acid derivative
OR Phosphoric acid ester OR Primary aliphatic amine OR Primary amine OR
Primary aromatic amine OR Quaternary ammonium salt OR Secondary alcohol
OR Secondary aliphatic amine OR Secondary amine OR Secondary aromatic
amine OR Sulfenic acid derivative OR Sulfonic acid OR Sulfonyl halide OR
Sulfuric acid derivative OR Tertiary alcohol OR Tertiary aliphatic amine
OR Tertiary amine OR Thiocarbonic acid derivative OR Thioether OR Thiol
OR Thiolactone OR Thiophosphoric acid amide OR Thiophosphoric acid
derivative OR Thiophosphoric acid ester OR Thiourea by Organic
functional groups, Norbert Haider (checkmol)
Domain
logical expression index: "h"
Referential
boundary: The
target chemical should be classified as Alcohol AND Aryl AND Azo AND
Benzyl AND Fused carbocyclic aromatic AND Naphtalene AND Phenol AND
Sulfonic acid by Organic Functional groups
Domain
logical expression index: "i"
Referential
boundary: The
target chemical should be classified as Alkane branched with quaternary
carbon OR Alkane, branched with tertiary carbon OR Alkene OR Alkoxy OR
AlkoxySilane OR Alkyl arenes OR Alkyne OR Allyl OR Alpha,beta
unsaturated aldehyde OR Benzotriazole OR Biphenyl OR Cycloalkane OR
Ether OR Fused heterocyclic aromatic OR Fused saturated heterocycles OR
Heterocyclic Phenol OR Heterocyclic spiro rings OR Isopropyl OR Ketone
OR No functional group found OR Phosphite ester OR Pyridine OR
Quinoline/ Isoquinoline OR Saturated heterocyclic fragment OR Silane OR
tert-Butyl OR Tetralin OR Thiophosphate OR Tin, organo OR Triazine OR
Unsaturated heterocyclic amine OR Unsaturated heterocyclic fragment by
Organic Functional groups
Domain
logical expression index: "j"
Parametric
boundary:The
target chemical should have a value of log Kow which is >= 0.269
Domain
logical expression index: "k"
Parametric
boundary:The
target chemical should have a value of log Kow which is <= 1.03
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed
- Dose descriptor:
- LD50
- Value:
- 35 636.23 mg/kg bw
- Quality of whole database:
- Data is of K2 level estimated using OECD QSAR Toolbox (v 3.3)
Additional information
Acute
toxicity: oral
No.
of studies of Chocolate Brown HT and its read across substance were
reviewed for acute toxicity by oral route from reliable sources having
Klimisch rating 2. The summary is represented as below:
For key study of target, Acute toxicity study was conducted on 10 female Evans albino mice for the Chocolate Brown HT (Disodium 4,4'-[[2,4-dihydroxy-5-(hydroxymethyl)-1,3-phenylene]bis(azo)]bisnaphthalene-1-sulphonate). Aqueous solution of the test material was administered at a dose volume of 10mL/Kg to the mice by oral intubation. On the basis of mortality, clinical signs observed and urinalysis performed, the LD50 for the given test material is determined to be >2000 mg/Kg. This value indicates that the substance is non-toxic by oral route as per the CLP criteria.
Similarly in supporting study of the target, Acute toxicity study was conducted on 5 male and female Porton SPF Wistar rats for the Chocolate Brown HT (Disodium 4,4'-[[2,4-dihydroxy-5-(hydroxymethyl)-1,3-phenylene]bis(azo)]bisnaphthalene-1-sulphonate). Aqueous solution of the test material was administered at a dose volume of 10mL/Kg to the rats by oral intubation. On the basis of mortality, clinical signs observed and urinalysis performed, the LD50 for the given test material is determined to be >2000 mg/Kg. This value indicates that the substance is non-toxic by oral route as per the CLP criteria.
Further in supporting data from Read across substance (CAS 3567-69-9), Acute oral toxicity study was conducted on mouse for the test material Azorubine (Disodium 4-hydroxy-3-[(4-sulphonatonaphthyl)azo]naphthalenesulphonate). The median lethal dose (LD50) for the substance was determined to be> 8000 mg/Kg. This value indicates that the substance is not toxic to mouse and hence is not classified under Acute oral toxicity.
Similarly, Acute oral toxicity study was conducted on rat for the same read across substance (CAS 3567-69-9) i.e. Azorubine (Disodium 4-hydroxy-3-[(4-sulphonatonaphthyl)azo]naphthalenesulphonate). The median lethal dose (LD50) for the substance was determined to be > 10000 mg/Kg. This value indicates that the substance is not toxic to rat and hence is not classified under Acute oral toxicity.
Considering all the above values, the target substance Chocolate Brown HT (Disodium 4,4'-[[2,4-dihydroxy-5-(hydroxymethyl)-1,3-phenylene]bis(azo)]bisnaphthalene-1-sulphonate) is not likely exhibit acute toxicity via oral route as per CLP regulation.
Acute
toxicity: inhalation
In
accordance with column 2 of Annex VIII of the REACH regulation, testing
by the inhalation route is appropriate if exposure of humans via
inhalation is likely taking into account the vapour pressure of the
substance. This chemical has a very low vapour pressure of 1.48E-33
Pascal at 25°C and thus exposure by the inhalation route for this
chemical is unlikely.
Acute
toxicity: dermal
The
acute toxicity by dermal route of Disodium 4,4’-(2,4-dihydroxy-5-
hydroxymethyl-1,3-phenylene bis-azo) di- (naphthalene-1-sulfonate) is
estimated using QSAR Toolbox version 3.3. The median lethal dose (LD50)
for the substance Disodium
4,4'-[[2,4-dihydroxy-5-(hydroxymethyl)-1,3-phenylene]bis(azo)]bisnaphthalene-1-sulphonate
is estimated to be 35636.23 mg/Kg in rabbits via dermal route. This
value indicates that the substance is not likely to exhibit toxicity by
dermal route as per the CLP criteria.
Justification for classification or non-classification
Based on the available information from reliable sources, the substance is likely to be non-toxic by oral and dermal route as per the CLP criteria.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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