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EC number: 219-983-4 | CAS number: 2591-76-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1988-05-16 to 1988-06-27
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 988
- Report date:
- 1988
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- Deviations:
- no
- GLP compliance:
- no
Test material
- Reference substance name:
- N,N-bis(2-methylpropyl)formamide
- EC Number:
- 219-983-4
- EC Name:
- N,N-bis(2-methylpropyl)formamide
- Cas Number:
- 2591-76-6
- Molecular formula:
- C9H19NO
- IUPAC Name:
- N,N-bis(2-methylpropyl)formamide
- Details on test material:
- - Name of the test substance used in the study report: Diisobutylformamid
- Purity: Please refer to section 1.2 for purity information.
Constituent 1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- Vienna White
- Details on test animals or tissues and environmental conditions:
- TEST ANIMALS
- Source: Gaukler, 6050 Offenbach/Main, Germany
- Age at study initiation:
- Weight at study initiation (mean): 2.92 kg
- Housing: Singly housing in stainless steel cages with wire mesh walk floors.
- Diet: Standardized animal laboratory diet (ca. 130 g/animal/day Kliba 341, Firma Klingentalmuehle AG, 4303 Kaiseraugstr, Switzerland)
- Water: About 250 mL tap water/animal/day
- Acclimation period: At least 8 days before the beginning of the study
- Animal identification. Ear tattoo
ENVIRONMENTAL CONDITIONS
- Temperature: 20 -24 °C
- Humidity: 30 - 70 %
- Photoperiod: 12 hours dark/ 12 hours light
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- other: untreated eye of the same animal
- Amount / concentration applied:
- 0.1 mL to the conjunctival sac of the right eye
- Duration of treatment / exposure:
- 24 h
- Observation period (in vivo):
- 21 d
- Number of animals or in vitro replicates:
- 3 males
- Details on study design:
- REMOVAL OF TEST SUBSTANCE
- The substance was not washed out
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- cornea opacity score
- Basis:
- mean
- Time point:
- other: 24, 48, 72 h
- Score:
- 1
- Max. score:
- 1
- Reversibility:
- not fully reversible within: 21 d
- Irritation parameter:
- iris score
- Basis:
- mean
- Time point:
- other: 24, 48, 72 h
- Score:
- 0.3
- Max. score:
- 1
- Reversibility:
- fully reversible within: 8 d
- Irritation parameter:
- conjunctivae score
- Basis:
- mean
- Time point:
- other: 24, 48, 72 h
- Score:
- 1.6
- Max. score:
- 2
- Reversibility:
- fully reversible within: 21 d
- Irritation parameter:
- chemosis score
- Basis:
- mean
- Time point:
- other: 24, 48, 72 h
- Score:
- 0.9
- Max. score:
- 2
- Reversibility:
- fully reversible within: 8 d
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #1
- Time point:
- other: 24, 48, 72 h
- Score:
- 1
- Max. score:
- 4
- Reversibility:
- fully reversible within: 21 d
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #2
- Time point:
- other: 24, 48, 72 h
- Score:
- 1
- Max. score:
- 4
- Reversibility:
- not fully reversible within: 21 d
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #3
- Time point:
- other: 24, 48, 72 h
- Score:
- 1
- Max. score:
- 4
- Reversibility:
- fully reversible within: 8 d
- Irritation parameter:
- iris score
- Basis:
- animal #1
- Time point:
- other: 24, 48, 72 h
- Score:
- 0.3
- Max. score:
- 2
- Reversibility:
- fully reversible within: 48 h
- Irritation parameter:
- iris score
- Basis:
- animal #2
- Time point:
- other: 24, 48, 72 h
- Score:
- 0.7
- Max. score:
- 2
- Reversibility:
- fully reversible within: 8 d
- Irritation parameter:
- iris score
- Basis:
- animal #3
- Time point:
- other: 24, 48, 72 h
- Score:
- 0
- Max. score:
- 2
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #1
- Time point:
- other: 24, 48, 72 h
- Score:
- 1.7
- Max. score:
- 3
- Reversibility:
- fully reversible within: 8 d
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #2
- Time point:
- other: 24, 48, 72 h
- Score:
- 2
- Max. score:
- 3
- Reversibility:
- fully reversible within: 21 d
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #3
- Time point:
- other: 24, 48, 72 h
- Score:
- 1
- Max. score:
- 3
- Reversibility:
- fully reversible within: 8 d
- Irritation parameter:
- chemosis score
- Basis:
- animal #1
- Time point:
- other: 24, 48, 72 h
- Score:
- 1
- Max. score:
- 4
- Reversibility:
- fully reversible within: 72 h
- Irritation parameter:
- chemosis score
- Basis:
- animal #2
- Time point:
- other: 24, 48, 72 h
- Score:
- 1.7
- Max. score:
- 4
- Reversibility:
- fully reversible within: 8 d
- Irritation parameter:
- chemosis score
- Basis:
- animal #3
- Time point:
- other: 24, 48, 72 h
- Score:
- 0
- Max. score:
- 4
Any other information on results incl. tables
Readings |
Animal |
Cornea |
Iris |
Conjunctiva |
Symptoms |
|||
op |
ar |
red |
sw |
di |
||||
1 h |
1 |
1 |
2 |
0 |
2 |
2 |
3 |
|
2 |
0 |
0 |
0 |
2 |
2 |
2 |
||
3 |
0 |
0 |
0 |
2 |
2 |
2 |
||
24 h |
1 |
1 |
4 |
1 |
2 |
2 |
2 |
|
2 |
1 |
4 |
0 |
2 |
1 |
1 |
||
3 |
1 |
4 |
0 |
1 |
0 |
1 |
||
48 h |
1 |
1 |
4 |
0 |
2 |
1 |
0 |
PC/RE/S/LC |
2 |
1 |
4 |
1 |
2 |
2 |
2 |
||
3 |
1 |
3 |
0 |
1 |
0 |
1 |
||
72 h |
1 |
1 |
4 |
0 |
1 |
0 |
0 |
PC/RE/S/LC |
2 |
1 |
4 |
1 |
2 |
2 |
2 |
||
3 |
1 |
2 |
0 |
1 |
0 |
0 |
||
8 days |
1 |
1 |
2 |
0 |
0 |
0 |
0 |
PC/RE/LC/MV |
2 |
1 |
2 |
0 |
2 |
0 |
2 |
||
3 |
0 |
0 |
0 |
0 |
0 |
0 |
||
15 days |
1 |
1 |
1 |
0 |
0 |
0 |
0 |
RE/LC/MV |
2 |
1 |
1 |
0 |
1 |
0 |
0 |
||
3 |
0 |
0 |
0 |
0 |
0 |
0 |
||
21 days |
1 |
0 |
0 |
0 |
0 |
0 |
0 |
RE/LC/MV |
2 |
1 |
1 |
0 |
0 |
0 |
0 |
||
3 |
0 |
0 |
0 |
0 |
0 |
0 |
||
Mean |
1 |
1.0 |
|
0.3 |
1.7 |
1.0 |
|
|
2 |
1.0 |
|
0.7 |
2.0 |
1.7 |
|
||
3 |
1.0 |
|
0.0 |
1.0 |
0.0 |
|
||
Mean |
|
1.0 |
|
0.3 |
1.6 |
0.9 |
|
|
LC - Loss of corneal tissue
MV - Marginal vascularization of the cornea
PC - Pupil contracted
RE - Small retractions in the eyelids
S - Suppuration
Applicant's summary and conclusion
- Interpretation of results:
- Category 1 (irreversible effects on the eye) based on GHS criteria
- Conclusions:
- Based in the effects of the test substance to the cornea, the test substance is regarded as causing irreversible damage to the eye.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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