Dialuminium tris[2-(2,4,5,7-tetrabromo-6-oxido-3-oxoxanthen-9-yl)-3,4,5,6-tetrachlorobenzoate]

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Justification for type of information:

As per mention in publication.

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Principles of method if other than guideline:

Determination of the inhibition of the mobility of daphnids using OECD guideline 202.

GLP compliance:

no

Specific details on test material used for the study:

- Name of test material :Dialuminium tris(2-(2,4,5,7-tetrabromo-6-oxido-3-oxoxanthen-9-yl)-3,4,5,6-tetrachlorobenzoate)
- Molecular formula :C20H4Br4Cl4O5.2/3Al
- Substance type: Organic
- Physical state: Solid

Analytical monitoring:

no

Details on sampling:

No data available

Vehicle:

no

Details on test solutions:

The stock solution 200 mg/L was prepared by dissolving dark red powder in reconstituted water. The test solutions of required concentrations were prepared by mixing the stock solution of the test sample in reconstututed test water.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM- Common name: water flea- Source:University of Chemistry and Technology Prague- Feeding during test: Without feeding

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Remarks on exposure duration:

No data available

Post exposure observation period:

No

Hardness:

no data

Test temperature:

20°C±1 °C

pH:

sample at concentration 200 mg/l:pH= 7.7 changed to pH=7.5during the test, control : pH=7.8 changed to pH=7.6 during the test,

Dissolved oxygen:

higher than 7.9 mg/1 both in the control and the sample

Salinity:

no data

Conductivity:

no data

Nominal and measured concentrations:

Nominal :0, 5, 10,25,50,100,200 mg/l

Details on test conditions:

TEST SYSTEM- No. of organisms per vessel: 5- No. of vessels per concentration (replicates): 4TEST MEDIUM / WATER PARAMETERS- Source/preparation of dilution water:The stock solution 200.0 mg/l was prepared by dissolving orange powder inreconstituted water.The stock solution was kept 5 min in ultrasonic bath.The test solutions of required concentrations were prepared by mixing the stock solution of the test sample inreconstituted test water.OTHER TEST CONDITIONS- Adjustment of pH:sample at concentration 200 mg/l:pH= 7.5 changed to pH=7.7during the test, control 1: pH=7.6 changed to pH=7.4 during the test, control 2: pH=7.7 didnot change during the test, control 3 : pH=7.7 changed to pH=7.6 during the test- Light intensity:darknessTEST CONCENTRATIONS- Test concentrations: 5,10,25,50,100,200 mg/l

Reference substance (positive control):

yes

Remarks:

K2Cr207

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

152.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 108.7-213.4

Details on results:

EC50 was calculated using nonlinear regression.

Results with reference substance (positive control):

- Results with reference substance valid?- EC50: 0.79 mg/l

Reported statistics and error estimates:

EC50 was calculated using nonlinear regression by the software Prism4.0 (GraphPadSoftware, Inc.,SanDiego CA)

Validity criteria fulfilled:

not specified

Conclusions:

On the basis of growth inhibition, the EC50 was 152.3 mg/L (95% C.I: 108.7-213.4 mg/L) after 48 hours of exposure in D. magna.

Executive summary:

An acute immobilisation test was used to test how a range of concentrations of CAS 15876-58-1 exerts different degrees of toxic effects on the swimming capability of Daphnia magna under otherwise identical test conditions. The test was performed in close resemblance to OECD guideline 202 by ABITEC in Prague. The standardized protocol used to obtain the herein described results was by the testing laboratory referred to as “CSN EN ISO 6341”. The testing aim was to determine a EC50 after 48 hours of exposure to D. magna. The 48 hours EC50 was by the testing laboratory defined as the concentration estimated to immobilise 50 per cent of theDaphniaafter 48 hours of exposure. The stock solution used for the exposure assessment was prepared by dissolving dark red powder in reconstituted water. The stock solution was kept at 5 min in an ultrasonic bath. The test exposure concentrations were prepared by mixing the stock solution of the test sample with reconstituted water. Daphnids were exposed to CAS 15876-58-1 in 50 ml beakers in a volume of 25 ml of liquid solution containing both the chemical and media as specified in OECD 202. The beakers were placed in a temperature controlled room at 20±1 degrees Celsius. The D. magna (age ≤24) used for the test had been breed at ABITEC. The breeding stock of D. magna originated from University of Technology in Prague. The animals were exposed to medium (i.e.a beaker containing only medium) and/or the tested chemical during 48 hours (±1 hour). None of the exposed animal’s immobilization were affected by exposure to only medium. The nominal concentrations used were: 5, 10, 25, 50, 100 and 200 mg/L. There were 5 Daphnia per test vessels and 4 replicates per concentration. The pH in test vessels were 7.5-7.8 mg/L. The positive control/reference substance used in the tested showed an expected result and gave a EC50 that corresponded to previous exposures with this chemical in D. magna. Immobilisation effects in D. manga by chemical exposure was evident after 48 hours in exposure concentrations above 10 mg/L. The EC50 was defined as a concentration that immobilizes 50% of the exposed D. magna. The % of immobilization in D. magna after 48 hours of exposure to CAS 15876-39-8 were used in a nonlinear regression by Graphpad Software Prism 4.0 (San Diego, US). The EC50 was estimated based on the nonlinear regression. In conclusion, the EC50 for CAS 15876-58-1 was 152.3 mg/L (95% C.I: 108.7-213.4 mg/L) after 48 hours of exposure in D. magna.

Description of key information

An acute immobilisation test was used to test how a range of concentrations of CAS 15876-58-1 exerts different degrees of toxic effects on the swimming capability of Daphnia magna under otherwise identical test conditions. The test was performed in close resemblance to OECD guideline 202 by ABITEC in Prague. The standardized protocol used to obtain the herein described results was by the testing laboratory referred to as “CSN EN ISO 6341”. The testing aim was to determine a EC50 after 48 hours of exposure to D. magna. The 48 hours EC50 was by the testing laboratory defined as the concentration estimated to immobilise 50 per cent of the Daphnia after 48 hours of exposure. The stock solution used for the exposure assessment was prepared by dissolving dark red powder in reconstituted water. The stock solution was kept at 5 min in an ultrasonic bath. The test exposure concentrations were prepared by mixing the stock solution of the test sample with reconstituted water. Daphnids were exposed to CAS 15876-58-1 in 50 ml beakers in a volume of 25 ml of liquid solution containing both the chemical and media as specified in OECD 202. The beakers were placed in a temperature controlled room at 20±1 degrees Celsius. The D. magna (age ≤24) used for the test had been breed at ABITEC. The breeding stock of D. magna originated from University of Technology in Prague. The animals were exposed to medium (i.e.a beaker containing only medium) and/or the tested chemical during 48 hours (±1 hour). None of the exposed animal’s immobilization were affected by exposure to only medium. The nominal concentrations used were: 5, 10, 25, 50, 100 and 200 mg/L. There were 5Daphniaper test vessels and 4 replicates per concentration. The pH in test vessels were 7.5-7.8 mg/L. The positive control/reference substance used in the tested showed an expected result and gave a EC50 that corresponded to previous exposures with this chemical inD. magna. Immobilisation effects inD. mangaby chemical exposure was evident after 48 hours in exposure concentrations above 10 mg/L. The EC50 was defined as a concentration that immobilizes 50% of the exposedD. magna. The % of immobilization in D. magna after 48 hours of exposure to CAS 15876-39-8 were used in a nonlinear regression by Graphpad Software Prism 4.0 (San Diego, US). The EC50 was estimated based on the nonlinear regression. In conclusion, the EC50 for CAS 15876-58-1 was 152.3 mg/L (95% C.I: 108.7-213.4 mg/L) after 48 hours of exposure inD. magna.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

152.3 mg/L

Additional information

Based on the various experimental data for the target chemical study have been reviewed to determine the toxic nature of Dialuminium tris[2-(2,4,5,7-tetrabromo-6-oxido-3-oxoxanthen-9-yl)-3,4,5,6-tetrachlorobenzoate] (15876 -58 -1). The studies are as mentioned below:

In the first key study for 15876 -58 -1, from report, An acute immobilisation test was used to test how a range of concentrations of CAS 15876-58-1 exerts different degrees of toxic effects on the swimming capability ofDaphnia magnaunder otherwise identical test conditions. The test was performed in close resemblance to OECD guideline 202 by ABITEC in Prague. The standardized protocol used to obtain the herein described results was by the testing laboratory referred to as “CSN EN ISO 6341”. The testing aim was to determine a EC50 after 48 hours of exposure toD. magna. The 48 hours EC50 was by the testing laboratory defined as the concentration estimated to immobilise 50 per cent of theDaphniaafter 48 hours of exposure. The stock solution used for the exposure assessment was prepared by dissolving dark red powder in reconstituted water. The stock solution was kept at 5 min in an ultrasonic bath. The test exposure concentrations were prepared by mixing the stock solution of the test sample with reconstituted water.Daphnidswere exposed to CAS 15876-58-1 in 50 ml beakers in a volume of 25 ml of liquid solution containing both the chemical and media as specified in OECD 202. The beakers were placed in a temperature controlled room at 20±1 degrees Celsius. TheD. magna(age ≤24) used for the test had been breed at ABITEC. The breeding stock ofD. magnaoriginated from University of Technology in Prague. The animals were exposed to medium (i.e.a beaker containing only medium) and/or the tested chemical during 48 hours (±1 hour). None of the exposed animal’s immobilization were affected by exposure to only medium. The nominal concentrations used were: 5, 10, 25, 50, 100 and 200 mg/L. There were 5Daphniaper test vessels and 4 replicates per concentration. The pH in test vessels were 7.5-7.8 mg/L. The positive control/reference substance used in the tested showed an expected result and gave a EC50 that corresponded to previous exposures with this chemical inD. magna. Immobilisation effects inD. mangaby chemical exposure was evident after 48 hours in exposure concentrations above 10 mg/L. The EC50 was defined as a concentration that immobilizes 50% of the exposedD. magna. The % of immobilization inD. magnaafter 48 hours of exposure to CAS 15876-39-8 were used in a nonlinear regression by Graphpad Software Prism 4.0 (San Diego, US). The EC50 was estimated based on the nonlinear regression. In conclusion, the EC50 for CAS 15876-58-1 was 152.3 mg/L (95% C.I: 108.7-213.4 mg/L) after 48 hours of exposure inD. magna.

In the another support study for RA chemical rose bengal (632 -68 -8),1977. Short term toxicity study of rose bengal to Artemia Salina was carried out for 24-48 hrs. The test chemical conc. used for the study was 1049.84 mg/l, respectively. A. salina eggs (encysted dried gastrulae) were commercially obtained, and were stored at -20°C, Eggs used in experiments were washed and stored at room temperature in a desiccator over anhydrous granular CaCl, . Larvae were obtained by incubating eggs in petri dishes containing muslin-filtered sea water at 30°C for 24 hours. The larvae were separated from shells, dead larvae and unhatched eggs by their phototactic movements toward a light source. Food dyes of various concentrations were placed in a petri dish, and sea water containing 20 to 30 larva ewas added. After this was incubated at 30°Cfor 24 hours and 48 hours, larvae surviving were measured by direct count. The same method was tested from 5 to 6 times for each concentration, and the death rate was calculated. The study was performed under static conditions for 24 – 48 hrs at 30°C. Death was assumed to have occurred when there was no movement. The death rate was defined as the average of the percentage of deaths observed for 24 hours and 48 hours. Based on death rate or mortality of test organism, the 48 hr LC100 value was found to be 1049.84 mg/l. According to the result the test chemical rose bengal was not classified according to the CLP regulations.

In the another supporting study for RA chemical 6441 -77 -6, Short term toxicity study of Phloxin to Artemia Salina was carried out for 24-48 hrs. The test chemical conc. used for the study was 792.96 mg/l, respectively. A. salina eggs (encysted dried gastrulae) were commercially obtained, and were stored at -20°C, Eggs used in experiments were washed and stored at room temperature in a desiccator over anhydrous granular CaCl, . Larvae were obtained by incubating eggs in petri dishes containing muslin-filtered sea water at 30°C for 24 hours. The larvae were separated from shells, dead larvae and unhatched eggs by their phototactic movements toward a light source. Food dyes of various concentrations were placed in a petri dish, and sea water containing 20 to 30 larva ewas added. After this was incubated at 30°C for 24 hours and 48 hours, larvae surviving were measured by direct count. The same method was tested from 5 to 6 times for each concentration, and the death rate was calculated.The study was performed under static conditions for 24 – 48 hrs at 30°C. Death was assumed to have occurred when there was no movement. The death rate was defined as the average of the percentage of deaths observed for 48 hours. Based on death rate or mortality of test organism, the LC 100 value was 792.96 mg/l. The chemical Phloxin was considered as non toxic and not classified according to the CLP regulation.

Based on the data for target chemical considering RA chemical and target chemical, Dialuminium tris[2-(2,4,5,7-tetrabromo-6-oxido-3-oxoxanthen-9-yl)-3,4,5,6-tetrachlorobenzoate] was consider as non toxic and can be classified as not classified as per CLP classification criteria.