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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Link to relevant study record(s)

Description of key information

Two disregarded studies were available, examining the test substance in a non-pure quality. 

Key value for chemical safety assessment

Additional information

Disregarded study inhibitory effect

The inhibitory effect of the test substance on the cell multiplication of the bacterium Pseudomonas putida was investigated in a 16-hour static test according to DIN EN ISO 10712 (February 1996). The test substance was not tested in a pure quality. The test substance was tested in the concentration range between 1250 and 10000 mg/L (nominal). The dilution factor was 2. The following results show the nominal concentrations of the test sample that causes after 16 h an inhibition of bacterial cell multiplication by 10% (EC10), 50% (EC50) and 90% (EC90) compared to the untreated control: EC10 (16 h) => 10000 mg/L (nominal), EC50 (16 h) > 10000 mg/L (nominal), EC90 (16) > 10000 mg/L (nominal). The validity criteria were fulfilled by the test.

Disregarded study respiratory inhibition test

The toxicity to microorganisms of the test item was tested in a study according to OECD guideline 209. The test substance was not tested in pure quality. The respiratory inhibition was tested with activated sludge predominantly domestic sewage. A static teset was performed with nominal concentrations of 1000, 500 and 100 mg/L. As reference substance 3,5-Dichlorphenol was used. The test substance was tested in only pure quality. The EC20 value was determined to be ca. 900 mg/L. No EC50 value were measured.