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EC number: 270-393-3 | CAS number: 68427-35-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Bioaccumulation: aquatic / sediment
Administrative data
- Endpoint:
- bioaccumulation in aquatic species: fish
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data is from J-CHECK authoritative database
Data source
Reference
- Reference Type:
- other: authoritative database
- Title:
- Bioaccumulation: aquatic/sediment
- Author:
- National Institute of Technology and Evaluation.
- Year:
- 2 017
- Bibliographic source:
- Japan chemicals collaborative knowledge database (J-check), 2017
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 305 (Bioconcentration: Flow-through Fish Test)
- Qualifier:
- according to guideline
- Guideline:
- other: "Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances"
- Principles of method if other than guideline:
- Bioaccumulation study was conducted on test organism Cyprinus carpio for 28 days for evaluating the bioconcentration factor (BCF value) of 2-(3-diethylamino-6-diethylazaniumylidene-xanthen-9-yl)-5-sulfo-benzenesulfonate. The study was performed according to OECD Guideline 305 (Bioconcentration: Flow-through Fish Test) and other guideline "Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances", respectively.
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Hydrogen 3,6-bis(diethylamino)-9-(2,4-disulphonatophenyl)xanthylium, sodium salt
- EC Number:
- 222-529-8
- EC Name:
- Hydrogen 3,6-bis(diethylamino)-9-(2,4-disulphonatophenyl)xanthylium, sodium salt
- Cas Number:
- 3520-42-1
- Molecular formula:
- C27H30N2O7S2.Na
- IUPAC Name:
- 2-(3-diethylamino-6-diethylazaniumylidene-xanthen-9-yl)-5-sulfo-benzenesulfonate
- Test material form:
- solid
Constituent 1
- Specific details on test material used for the study:
- - Name of test material (IUPAC name): 2-(3-diethylamino-6-diethylazaniumylidene-xanthen-9-yl)-5-sulfo-benzenesulfonate
- Common name: C. I. Acid Red 52
- Molecular formula: C27H30N2O7S2.Na
- Molecular weight: 580.6551 g/mol
- Smiles notation: CCN(CC)c1ccc2c(c1)[o+]c3cc(ccc3c2c4ccc(cc4S(=O)(=O)[O-])S(=O)(=O)[O-])N(CC)CC.[Na+]
- InChI: 1S/C27H30N2O7S2.Na/c1-5-28(6-2)18-9-12-21-24(15-18)36-25-16-19(29(7-3)8-4)10-13-22(25)27(21)23-14-11-20(37(30,31)32)17-26(23)38(33,34)35;/h9-17H,5-8H2,1-4H3,(H-,30,31,32,33,34,35);/q;+1/p-1
- Substance type: Organic
- Physical state: solid
- Purity: 84.4%
- Water solubility: ≥ 100 g/l (20°C) - Radiolabelling:
- not specified
Test solutions
- Vehicle:
- not specified
Test organisms
- Test organisms (species):
- Cyprinus carpio
Study design
- Route of exposure:
- aqueous
- Test type:
- static
- Water / sediment media type:
- natural water: freshwater
- Total exposure / uptake duration:
- 28 d
Test conditions
- Details on test conditions:
- RANGE-FINDING / PRELIMINARY STUDY
- Test concentrations : LC50(96h) > 200 mg/L, Rice fish (Oryzias latipes) - Nominal and measured concentrations:
- Nominal concentrations:
1st concentration area: 1.69 mg/l (1690 µg/l)
2nd concentration area: 0.169 mg/l (169 µg/l) - Reference substance (positive control):
- not specified
Results and discussion
Lipid contentopen allclose all
- Lipid content:
- 5.09 %
- Time point:
- start of exposure
- Lipid content:
- 6 %
- Time point:
- end of exposure
Bioaccumulation factoropen allclose all
- Conc. / dose:
- 1.69 mg/L
- Type:
- BCF
- Value:
- <= 0.57 L/kg
- Basis:
- total lipid content
- Time of plateau:
- 28 d
- Remarks on result:
- other: Other details not known
- Conc. / dose:
- 0.169 mg/L
- Type:
- BCF
- Value:
- <= 5.3 L/kg
- Basis:
- total lipid content
- Time of plateau:
- 28 d
- Remarks on result:
- other: Other details not known
Any other information on results incl. tables
Bioaccumulation test results:
10 d |
14 d |
19 d |
24 d |
28 d |
||
1st concentration area |
Test substance concentration (microg/L) |
1610 |
1670 |
1620 |
1640 |
1650 |
Bioconcentration factor (L/kg) |
<= 0.57 |
<= 0.57 |
<= 0.57 |
<= 0.57 |
<= 0.57 |
|
<= 0.57 |
<= 0.57 |
<= 0.57 |
<= 0.57 |
<= 0.57 |
||
2nd concentration area |
Test substance concentration (microg/L) |
181 |
172 |
176 |
177 |
170 |
Bioconcentration factor (L/kg) |
<= 5.3 |
<= 5.3 |
<= 5.3 |
<= 5.3 |
<= 5.3 |
|
<= 5.3 |
<= 5.3 |
<= 5.3 |
<= 5.3 |
<= 5.3 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The bioconcentration factor (BCF value) of substance 2-(3-diethylamino-6-diethylazaniumylidene-xanthen-9-yl)-5-sulfo-benzenesulfonate on Cyprinus carpio was determined to be ≤ 0.57 L/Kg at a conc. of 1.69 mg/l and ≤ 5.3 L/Kg at a conc. of 0.169 mg/l, respectively.
- Executive summary:
Bioaccumulation study was conducted on test organism Cyprinus carpiofor 28 days for evaluating the bioconcentration factor (BCF value) of 2-(3-diethylamino-6-diethylazaniumylidene-xanthen-9-yl)-5-sulfo-benzenesulfonate (CAS no. 3520-42-1).The study was performed according toOECD Guideline 305 (Bioconcentration: Flow-through Fish Test) and other guideline"Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances", respectively. Cyprinus carpio was used as a test organism for the study. Test chemical nominal conc. used for the study were1.69 mg/land 0.169 mg/l, respectively. Analytical method involve the recovery ratio:Fish : 89.6 % - Limit of quantitation : Test water : 1st concentration area : 83 microg/L 2nd concentration area : 8.3 microg/L Fish: 940 ng/g.Range finding study involve the LC50(96h) > 200 mg/LonRice fish (Oryzias latipes). Lipid content of the test organism Cyprinus carpio was determined to be 5.09% and 6% at the start and end of the exposure. The bioconcentration factor (BCF value) of substance 2-(3-diethylamino-6-diethylazaniumylidene-xanthen-9-yl)-5-sulfo-benzenesulfonate on Cyprinus carpio was determined to be≤ 0.57 L/Kg at a conc. of 1.69 mg/l and ≤ 5.3 L/Kg at a conc. of 0.169 mg/l, respectively, which does not exceed the bioconcentration threshold of 2000, indicating that the chemical 2-(3-diethylamino-6-diethylazaniumylidene-xanthen-9-yl)-5-sulfo-benzenesulfonate is not expected to bioaccumulate in the food chain.
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