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EC number: 415-490-5 | CAS number: 141773-73-1 HELVETOLIDE
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Adsorption / desorption
Administrative data
Link to relevant study record(s)
- Endpoint:
- adsorption / desorption: screening
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 28 May 2012 to 30 May 2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- This study was performed according to EU Method C.19 and OECD Guideline 121 with GLP statement. This study is well documented and all validity criteria were fulfilled. No deviations were observed.
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
- Deviations:
- no
- Principles of method if other than guideline:
- Not applicable
- GLP compliance:
- yes (incl. QA statement)
- Type of method:
- HPLC estimation method
- Media:
- soil
- Specific details on test material used for the study:
- - Storage condition of test material: Room temperature, dry area, unopened containers, optimum temperature 11-25°C
- Radiolabelling:
- no
- Test temperature:
- 25°C (Column temperature)
- Details on study design: HPLC method:
- EQUIPMENT
- Apparatus: HPLC System
- Type: Agilent 1200 Series Liquid Chromatograph.
- Type, material and dimension of analytical (guard) column: Column = Hyperclone CN (CPS) (25 cm x 4.6 mm id)
- Detection system: UV detector wavelength (210 nm)
MOBILE PHASES
- Type: Acetonitrile:water (55:45 v/v)
- pH: no data
- Solutes for dissolving test and reference substances: HPLC mobile phase.
DETERMINATION OF DEAD TIME
- Method: by inert substances which are not retained by the column (formamide)
REFERENCE SUBSTANCES
- Identity: Acetanilide, Nitrobenzene, Naphthalene, 2-Methylnaphthalene, Anthracene, DDT.
DETERMINATION OF RETENTION TIMES
- Quantity introduced in the column: Injection volume 20 µL; Flow rate 1.0 mL/min.
REPETITIONS
- Number of determinations: 2
EVALUATION
- Calculation of capacity factors k' = (tr - t0) / t0, which tr = retention time (min) and t0 = dead time (min).
- Determination of the log Koc value = (log k' - A) / B, which A = intercept of the calibration curve and B = slope of the calibration curve. - Details on sampling:
- Not applicable
- Details on matrix:
- Not applicable
- Details on test conditions:
- Not applicable
- Computational methods:
- Not applicable
- Key result
- Type:
- log Koc
- Value:
- 4.3 dimensionless
- Temp.:
- 25 °C
- Key result
- Type:
- Koc
- Value:
- 21 000 L/kg
- Temp.:
- 25 °C
- Details on results (HPLC method):
- - Retention times of reference substances used for calibration: See table 5.4.1/1 in "Any other information on results incl. tables".
- Details of fitted regression line (log k' vs. log Koc): log Koc = 4.49*log k' + 4.07 (r² = 0.94)
- Graph of regression line attached: Yes. See in "Illustration"
- Average retention data for test substance: See table 5.4.1/1 in "Any other information on results incl. tables". - Adsorption and desorption constants:
- Not applicable
- Recovery of test material:
- Not applicable
- Concentration of test substance at end of adsorption equilibration period:
- Not applicable
- Concentration of test substance at end of desorption equilibration period:
- Not applicable
- Transformation products:
- not measured
- Details on results (Batch equilibrium method):
- Not applicable
- Statistics:
- Not applicable
- Validity criteria fulfilled:
- yes
- Conclusions:
- The substance was determined to have a log Koc value of 4.3 (equivalent to Koc = 21000 L/kg).
- Executive summary:
This study was performed according to OECD Guideline 121 and EU Method C.19 with GLP statement, to assess the adsorption coefficient of the test substance. The test utilized a high performance liquid chromatograph. A commercially available analytical column packed with a solid phase containing a moderately polar stationary phase with lipophilic and polar moieties was used.
In order to correlate the measured capacity factor (k') of the test substance with its Koc, six reference materials were selected from a list of recommended compounds. These reference compounds were selected to have log Koc values between 1.3 and 5.6. An internal standard (formamide) with no retention on the HPLC column was used to determine the deadtime of the HPLC system.
As the slope of the calibration curve for the reference standards showed good first order correlation and as the retention times between duplicate injections for each solution was consistent, the HPLC method was considered valid for the determination of adsorption coefficient.
All validity criteria were fulfilled.
The substance was determined to have a log Koc value of 4.3 (equivalent to Koc = 21000 L/kg).
Reference
Table 5.4.1/1: Determination of log Koc for the test substance
Sample |
Component |
tr (min) |
k’ |
Log k’ |
Log Koc |
Standard Run A (t0 = 2.834 min) |
Acetanilide Nitrobenzene Naphthalene 2-methylnaphthalene Anthracene DDT |
3.401 4.181 4.872 5.172 5.765 8.640 |
0.200 0.475 0.719 0.825 1.034 2.049 |
-0.699 -0.323 -0.143 -0.084 0.015 0.311 |
1.3 2.4 2.8 3.9 4.4 5.6 |
Sample A (t0 = 2.831 min) |
Test substance |
6.062 |
1.141 |
0.057 |
4.3 |
Sample B (t0 = 2.832 min) |
Test substance |
6.064 |
1.141 |
0.057 |
4.3 |
Standard Run B (t0 = 2.832 min) |
Acetanilide Nitrobenzene Naphthalene 2-methylnaphthalene Anthracene DDT |
3.400 4.180 4.871 5.171 5.764 8.642 |
0.201 0.476 0.720 0.826 1.035 2.052 |
-0.698 -0.322 -0.143 -0.083 0.015 0.312 |
1.3 2.4 2.8 3.9 4.4 5.6 |
Description of key information
OECD Guideline 121, EU Method C.19, GLP, key study, validity 1:
log Koc = 4.3; Koc = 21000
Immobile in soils (according to P.J. McCall et al., 1980)
Key value for chemical safety assessment
- Koc at 20 °C:
- 21 000
Additional information
One valid key study is available to assess the adsorption coefficient of the registered substance. This study was performed according to OECD Guideline 121 and EU Method C.19 with GLP statement. The test utilized a high performance liquid chromatograph. A commercially available analytical column packed with a solid phase containing a moderately polar stationary phase with lipophilic and polar moieties was used.
In order to correlate the measured capacity factor (k') of the substance with its Koc, six reference materials were selected from a list of recommended compounds. These reference compounds were selected to have log Koc values between 1.3 and 5.6. An internal standard (formamide) with no retention on the HPLC column was used to determine the deadtime of the HPLC system.
All validity criteria were fulfilled.
The substance was determined to have a log Koc value of 4.3 (equivalent to Koc = 21000). This result indicates that the registered substance is immobile in soils (according to P.J. McCall et al., 19801).
1McCall P.J., Swann RL, Laskowski D.A., Unger SM, Vrona SA and Dishburger H.J., (1980), “Estimation of chemical mobility in soil from liquid chromatographic retention times”, Bull Environ Contam Toxicol 24 (2) 190 -5.
Mobility classification scheme:
Range of Koc |
Mobility class |
0 - 50 |
Very high |
50 - 150 |
High |
150 - 500 |
Medium |
500 - 2000 |
Low |
2000 - 5000 |
Slightly |
> 5000 |
Immobile |
[LogKoc: 4.3]
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.