Fatty acids, rape-oil, Me esters

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

ISO 10253 (Water quality - Marine Algal Growth Inhibition Test with Skeletonema costatum and Phaeodactylum tricornutum)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Fatty acids, rape-oil, Me esters

EC Number:

287-828-8

EC Name:

Fatty acids, rape-oil, Me esters

Cas Number:

85586-25-0

Molecular formula:

Unspecified

IUPAC Name:

methyl (11Z)-icos-11-enoate; methyl (13Z)-docos-13-enoate; methyl (9E)-octadec-9-enoate; methyl (9Z,12Z)-octadeca-9,12-dienoate

Details on test material:

- Name of test material (as cited in study report): Rapeseed oil, fatty acid ester
- Physical state: yellowish liquid
- Analytical purity: 100%

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Since the test substance is poorly soluble in water WAFs were prepared.

Test organisms

Test organisms (species):

Skeletonema costatum

Study design

Test type:

static

Water media type:

saltwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Test temperature:

19.1 - 21.9

pH:

7.91 - 8.26

Salinity:

according to guideline

Nominal and measured concentrations:

Nominal: 3.2, 10, 18, 32 and 100 mg/L loading rate WAFs

Details on test conditions:

TEST SYSTEM
- Initial cells density: 10065 cells/mL
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 4

GROWTH MEDIUM
- Standard medium used: yes

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ISO medium

OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Photoperiod: continuous light
- Light intensity and quality: 7390 - 8190 lux

EFFECT PARAMETERS MEASURED: Cell number was counted after 72h exposure time.
- Determination of cell concentrations: Haemocytometer, Sedgewick-Rafter cell

TEST CONCENTRATIONS
- Range finding study: yes
- Test concentrations: 1, 10, 100 and 1000 mg/L loading rate WAFs
- Results used to determine the conditions for the definitive study: EC100 at 100 mg/L loading rate WAF

Reference substance (positive control):

yes

Remarks:

3,5-Dichlorophenol

Results and discussion

Effect concentrations

Duration:

72 h

Dose descriptor:

EL50

Effect conc.:

33.92 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

(WAF)

Basis for effect:

growth rate

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The marine algae Skeletonema costatum showed a 72h-EC50 value of 33.92 mg/L loading rate WAF in a toxicity test performed according to GLP and ISO guideline 10253.