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Diss Factsheets
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EC number: 240-032-4 | CAS number: 15894-70-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 993
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 472 (Genetic Toxicology: Escherichia coli, Reverse Mutation Assay)
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Test material form:
- solid: crystalline
- Details on test material:
- The dose preparations were corrected for purity (68.9%)
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- E. coli WP2
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- Liver S9 from phenobarbital/beta-naphthaflavone induced male rats
- Test concentrations with justification for top dose:
- 5000, 2500, 1000, 500, 200, 100 ug/plate
- Vehicle / solvent:
- DMSO
Controls
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- N-ethyl-N-nitro-N-nitrosoguanidine
- mitomycin C
- other: Acridine, 2-aminoanthracene and Daunamycin
- Details on test system and experimental conditions:
- HMBDA was assayed using standard plate incorporation protocol both in the presence and absence of liver S9-mix prepared from phenobarbital/beta-naphthaflavone induced rats. HMBDA was subsequently re-tested in all 6 strains over the same dose range. The +S9 phase of the second assay was conducted using the preincubation protocol.
- Evaluation criteria:
- Test data are considered valid if: (a) the concurrent solvent control data are acceptable (b) the positive control data show unequivocal positive responses (c) at least the lowest test compound dose shows no evidance of toxicity and at least 3 test doses show significant toxicity. Failure of one or more test strain/S9 combinations does not invalidate the data for the remainder of a concurrent experiment. A positive response is achieved when (a) a statistically significant dose related increase in mean number of revertant colonies is obtaines, and/or (b) a 2 fold or greater increase in the mean number of revertant colonies (compared to the concurrent solvent plate) which is statistically signigicant is observed at at least 1 dose level. A negative result is achieve when (a) there is no statistically significant dose related increase in mean number of revertant colonies per plate and (b) in the absence of any such dose response, no increase in colony numbers is observed which exceeds 2x concurrent solvent control. For a positive response to be considered unequivocal i.e. mutagenic the observed effect muct be consistently reproducible.
- Statistics:
- An assessment of statistical significance was carried out using a one tailed student's t test. The corresponding probability for each dose level was derived by computer using the appropriate degrees of freedom. Values of p<0.01 are treated as significant, with calues of 0.01
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity, but tested up to precipitating concentrations
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity, but tested up to precipitating concentrations
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity, but tested up to precipitating concentrations
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- In 2 separate assays HMBDA did not induce any significant reproducible increases in the observed number of revertant colonies in strains TA1535, TA1537, TA98, TA100, WP2P and WP2P uvrA either in the presence or absence of S9. The positive controls for each experiment induced the expected responses indicating the strains were responding satisfactorily in each case.
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
HMBDA was considered non-mutagenic to bacterial in vitro.
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Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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