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EC number: 206-557-8 | CAS number: 354-33-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 100 mg/L
Additional information
The assessment of the possible aquatic toxicity of HFC 125 has been carried out by considering available data on structurally analogue substances.
Experimental data for acute toxicity on Daphnia magna are available for HFC 134a (1,1,1,2 -tetrafluoroethane), HFC 143a (1,1,1 -trifluoroethane), HFC 245fa (1,1,1,3,3-pentafluoropropane) and HFC 365mfc (1,1,1,2,2 -pentafluorobutane).
In all cases studies were conducted in closed systems to avoid substance volatilisation.
All substances showed a low toxicity to D. magna. 48 hour-EC50 could be identified only for HFC 134a (LC50 = 980 mg/l) and for HFC 143a (LC50 = 300 mg/l), as low immobility was observed up to the highest tested concentrations for the other substances. Overall, a 48 -hour LC50= 100 mg/l (i.e. the lowest experimental value determined for sturctural analogs) can be considered as a conservative estimation of the HFC 125 acute toxicity to aquatic invertebrates.
HFC 245fa: Pentafluoropropane was tested for acute toxicity in D. magna following OECD Guidelines. Water fleas were exposed for 48 consecutive hours to a nominal limit concentration of 100 mg/l. A concurrent negative control group was included in the test. The test substance concentration was monitored at the test initiation and termination. The average measure concentration was 97.9 mg/l HFC 245fa. No immobility was observed in the control and treatment groups. A 48 -h EC50 > 100 mg/l was determined.
HFC365mfc: The acute toxicity of pentafluorobutane to Daphnia magna was tested following OECD Guidelines. Water fleas (3 replicate of 10 organisms/concentration) were exposed to 0, 100, 150, 200 mg/l pentafluorobutane for 48 hours in a closed system to avoid the test substance evaporation. The substance concentration in the samples was monitored at the test initiation and termination. A difference of less than 20% was observed among the nominal and measure concentrations.
No immobility was observed in controls. Some immobile water fleas were observed at 150 and 200 mg/l (6.7 and 13% of immobility, respectively), but the NOEC calculated by means of the Fisher's exact test was 200 mg/l. A NOEC of 100 mg/l was set for sublethal effects (water fleas were lying at the bottom of the flasks after 48 h exposure to 150 and 200 mg/l). A 48 -h EC50> 200 mg/l was determined.
HFC 134a: The 48-hour EC50 for HFC-134a in Daphnia magna under static conditions was 980 mg/l. A steep concentration/immobility curve was observed, mean measured concentrations of 870 and 1,100 mg/l causing 0 and 100% immobility respectively.
HFC 143a: 48 -hour EC50 for daphnia magna was calculated to be 300 mg/l with 95% confidence limits of 260 to 350 mg/l.under static conditions at 20 deg. C. The mean measured concentrations in the test flasks ranged from 81 to 111% of the nominal concentrations.
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