Calcium fluoride

Bioaccumulation: aquatic / sediment

Link to relevant study record(s)

Reference

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2001-09-28 to 2002-01-28

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 305 (Bioconcentration: Flow-through Fish Test)

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Purity: 99.6%

Vehicle:

no

Test organisms (species):

Cyprinus carpio

Route of exposure:

aqueous

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

28 d

Details on test conditions:

RANGE-FINDING / PRELIMINARY STUDY
- Results used to determine the conditions for the definitive study: LC50 (96h) = 522 mg/L for rice fish (Oryzias latipes)

Nominal and measured concentrations:

5 mg/L (1st concentration area) and 0.5 mg/L (2nd concentration area)

Lipid content:

3.41 %

Time point:

start of exposure

Lipid content:

3.26 %

Time point:

end of exposure

Conc. / dose:

5 mg/L

Type:

BCF

Value:

<= 0.66 L/kg

Calculation basis:

steady state

Conc. / dose:

0.5 mg/L

Type:

BCF

Value:

<= 6.4 L/kg

Calculation basis:

steady state

		6d	12d	16d	22d	28d
1st area concentration	test substance concentration (mg/L)	4.65	5.12	4.97	4.18	4.69
	Bioconcentration factor (l/kg)	≤ 0.66	≤ 0.66	≤ 0.66	≤ 0.66	≤ 0.66
2nd area concentration	Test substance concentration (mg/L)	0.478	0.531	0.493	0.403	0.521
	Bioconcentration factor (l/kg)	≤ 6.4	≤ 6.4	≤ 6.4	≤ 6.4	≤ 6.4

Validity criteria fulfilled:

yes

Conclusions:

The BCF values established in a study with NaF are ≤ 0.66 L/kg when exposed to 5 mg NaF/L and < 6.4 L/kg when exposed to 0.5 mg NaF/L.

Executive summary:

A 28 -days bio-accumulation study was conducted according to OECD 305, a flow-through fish test. Cyprinus carpio was exposed to 5 and 0.5 mg NaF/L. The bioconcentration factors measured at steady state are ≤ 0.66 L/kg when exposed to 5 mg NaF/L and < 6.4 L/kg when exposed to 0.5 mg NaF/L.

Description of key information

They key study for the bioaccumulation endpoint is a 28 -days bio-accumulation study on the read-across source susbtance sodium fluoride (NaF) conducted according to OECD 305, a flow-through fish test. Cyprinus carpio was exposed to 5 and 0.5 mg NaF/L. The bioconcentration factors measured at steady state are ≤ 0.66 L/kg when exposed to 5 mg NaF/L and < 6.4 L/kg when exposed to 0.5 mg NaF/L.

Conversion of the BCF value to reflect the difference in molecular weight between sodium fluoride and calcium difluoride is not relevant, as the bioaccumulated species is fluoride, not calcium difluoride.

A number of additional bioaccumulation studies are mentioned in RIVM's Integrated criteria document on fluorides. Even though the original study reports are not available today, the information in the review report is considered relevant and adequate to serve as supporting information. The BCF values reported for fluoride in the publication range from <1 to 7.5 in aquatic plants and from 50 to 150 in fish and crustacea.

Overall, the available data indicate that fluoride has low BCF values, and is considered not bioaccumulative in accordance with the REACH criteria.

Key value for chemical safety assessment

BCF (aquatic species):

6.4 L/kg ww