Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 246-836-1 | CAS number: 25321-14-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with acceptable restrictions (pure 2,6- dinitrotoluene tested; exposure for 6 h)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 991
- Report date:
- 1991
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- Deviations:
- yes
- Remarks:
- 6 h exposure
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- 2,6-dinitrotoluene
- EC Number:
- 210-106-0
- EC Name:
- 2,6-dinitrotoluene
- Cas Number:
- 606-20-2
- Molecular formula:
- C7H6N2O4
- IUPAC Name:
- 2-methyl-1,3-dinitrobenzene
- Details on test material:
- - Name of test material (as cited in study report): 2,6-dinitrotoluene
- Analytical purity: >99%
- Physical state: yellow solid
- Stability under test conditions: stable under normal conditions of use
- Storage condition of test material: in the dark at 4 °C
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Fischer 344
- Sex:
- male/female
Administration / exposure
- Route of administration:
- other: inhalation: vapour (lowest dose) and aerosol
- Type of inhalation exposure:
- nose only
- Vehicle:
- other: vapour: clean dried air; aerosol: acetone/polyethylenglycol 200
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: nose-only exposure chambers made of perspex
- Exposure chamber volume: 50 L
- Method of holding animals in test chamber: perspex restraining tubes which were attached at evenly spaced ports in the chamber. Each rat was restained in a forward position by an adjustable plastic stopper which also provided a seal for the tube.
- Source and rate of air: 25 L/min
- System of generating vapour: The yapour generator was constructed from a 1 litre 2-necked flask, an air inlet tube and glass distillation column which connected the flask to the inlet port on the exposure chamber. The flask was maintained at approx. 90 °C in a hot water bath. The glass column connecting the flask to the exposure chamber was filled with glass wool to act as a particulate trap and also allowed the test atmosphere to coll to approx. 26 °C before entering the exposure chamber.
- System of generating particulates/aerosols: The aerosol generator was designed to produce and maintain an atmosphere containing a high proportion of respirable droplets. The generator (made of stainless steel or glass) was comprised af a concentric jet atomiser and a plastic elutriation column. The solution of test substance in acetone was supplied to the generator from a syringe driven at a constant rate by a syringe pump. The compressed air supply to the generator was dried, filtered and oil-free.
- Method of particle size determination:
- Treatment of exhaust air: absolute filter
- Temperature: 22 - 27 °C
TEST ATMOSPHERE
- Brief description of analytical method used: Gas chromatography fitted with a flame ionisation detector was used. The quatification was calibrated with prepared 2,6-DNT standard solutions.
- Samples taken from breathing zone: yes; seven air samples were taken during each exposure
VEHICLE
- Composition of vehicle (if applicable): acetone and polyethylen glycol 200
- Concentration of test material in vehicle: 15 g 2,6-dinitrotoluene (2,6-DNT) and 5 g polyethylene glycol 200 were dissolved in sufficient acetone to make 100 mL of solution.
- Justification of choice of vehicle: Polyethylene glycol 200 was necessary to eliminate crystalisation of 2,6-DNT at the tip of the concentric jet atomiser.
TEST ATMOSPHERE
- Particle size distribution: Approx. 25% of the paricles were smaller than 1.0 µm size. Approx. 87 - 96% of the 2,6-DNT present in the chamber atmosphere was in the form of respirable particles (< 5.5 µm).
- MMAD (Mass median aerodynamic diameter) / GSD (Geometric st. dev.): MMAD 1.7 - 2.0 µm/GSD 2.26 - 2.95 - Analytical verification of test atmosphere concentrations:
- yes
- Duration of exposure:
- 6 h
- Concentrations:
- mean analytical concentrations: 0.026, 0.196, 0.473 and 0.694 mg/L air
- No. of animals per sex per dose:
- 5
- Control animals:
- yes
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: The rats were observed continuously for signs of reaction to the test substance during exposure and at least twice daily throughout the observation period. Clinical signs were recorded at the end of the chamber equilibration period (5 min), at 0.25, 0.5 and 1 h and then at hourly intervals during the exposure. During the observation period the clinical signs were recorded once in the morning and then as necessary.
- Necropsy of survivors performed: yes; also all animals that died as a result of exposure
- Other examinations performed:body weight (daily), food and water consumption (dails), organ weights (lung), other: blood samples were taken from the orbital sinuses from all rats at approx. 24 h before exposure and then at 1, 24 and 48 h post exposure. The samples were analysed for methemoglobin using the method of Van Kampen and Zijlstra, 1975. Additional samples were taken at seven days post exposure for selected groups.
Results and discussion
Effect levelsopen allclose all
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- 0.43 mg/L air (analytical)
- 95% CL:
- 0.23 - 0.63
- Exp. duration:
- 6 h
- Sex:
- male
- Dose descriptor:
- LC50
- Effect level:
- 0.24 mg/L air (analytical)
- 95% CL:
- 0.08 - 0.4
- Exp. duration:
- 6 h
- Sex:
- female
- Dose descriptor:
- LC50
- Effect level:
- 0.66 mg/L air (analytical)
- 95% CL:
- 0.49 - 0.83
- Exp. duration:
- 6 h
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- 0.36 mg/L air
- Exp. duration:
- 4 h
- Remarks on result:
- other: calculated from 6 h value (calculated value was adopted from the OECD SIDS 2004)
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.