Vanadium zirconium yellow baddeleyite

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

2000-03-13 to 2000-03-17

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: Significant methodological deficiencies - actual concentrations were not measured. - only a brief description of the test conditions is given

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2000

Report date:

2000

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

1992-07-17

Deviations:

yes

Remarks:

, please refer to the field "Rationale for reliability incl. deficiencies" above.

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Version / remarks:

1992

Deviations:

yes

Remarks:

, please refer to the field "Rationale for reliability incl. deficiencies" above.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Vanadium zirconium yellow baddeleyite

EC Number:

269-063-1

EC Name:

Vanadium zirconium yellow baddeleyite

Cas Number:

68187-01-9

Molecular formula:

V(x)Zr(1-x)O2 0,01≤x≤0,12

IUPAC Name:

divanadium(5+) zirconium(4+) heptaoxidandiide

Test material form:

solid: particulate/powder

Details on test material:

- Name of test material (as cited in study report): Vanadium-Zirconium Yellow
- Substance type: inorganic pigment
- Physical state: yellow powder
- Storage condition of test material: at room temperature in the dark
- Stability in water: at least 96 hours

Sampling and analysis

Analytical monitoring:

not specified

Details on sampling:

no data

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method:
The test solution was prepared at 100 mg/L applying magnetic stirring for 45 minutes followed by 1 minute of treatment with ultrasonic waves. The final test solution was a yellow suspension with some undissolved particles.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zebra-fish (Danio rerio, Teleostei, Cyprinidae)
- Source: Zodiac, proefacc. "De Haar Vissen", L.U. Wageningen, the Netherlands
- Length at study initiation (mean ± SD): 2.29 ± 0.11 cm
- Weight at study initiation (mean ± SD): 0.25 ± 0.04 g
- Characteristics: F1 from a single parent-pair bred in UV-treated water

Study design

Test type:

static

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Hardness:

218 mg CaCo3/L

Test temperature:

20.9 - 22.2°C

pH:

- Control (blank):
test start: 7.9
test end: 7.8
- Test vessel:
test start: 7.8
test end: 7.9

Dissolved oxygen:

- Control (blank):
test start: 10.0 mg O2/L
test end: 8.4 mg O2/L
- Test vessel:
test start: 9.8 mg O2/L
test end: 8.6 mg O2/L

Salinity:

not applicable

Nominal and measured concentrations:

Nominal concentration: 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 6 litres, all glass
- Aeration: solutions were gently aerated as oxygen concentrations tend to decrease below 6 mg/L.
- No. of organisms per vessel: 7
- Loading: 0.35 g fish per litre (i.e. 7 fish per 5 litres of test medium)

TEST MEDIUM / WATER PARAMETERS
- test water: ISO-medium (hardness: 218 mg CaCO3/L)

EFFECT PARAMETERS MEASURED
- incidence of mortality and total mortality

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Deposits were observed during the entire test period in the 100 mg/L solution.

Details on results:

Vanadium-Zirconium yellow induced no vissible effects in zebra-fish at 100 mg/L during the 96-hour test period.

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

LC50 (96 hours) > 100 mg/L
Under the conditions of the present test vanadium-Zirconium yellow induced no vissible effects in zebra-fish at 100 mg/L during the 96-hour test period.