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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Only short abstract available.

Data source

Reference
Reference Type:
other company data
Title:
Unnamed
Year:
1977
Report date:
1977

Materials and methods

Test guideline
Qualifier:
no guideline available
Guideline:
other: measured
GLP compliance:
not specified
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
Sodium [2-[(2,6-dichlorophenyl)amino]phenyl]acetate
EC Number:
239-346-4
EC Name:
Sodium [2-[(2,6-dichlorophenyl)amino]phenyl]acetate
Cas Number:
15307-79-6
Molecular formula:
C14H11Cl2NO2.Na
IUPAC Name:
sodium {2-[(2,6-dichlorophenyl)amino]phenyl}acetate

Method

Target gene:
his operon
Species / strain
Species / strain / cell type:
S. typhimurium, other: not further specified
Metabolic activation:
with and without
Metabolic activation system:
S9 mix
Test concentrations with justification for top dose:
0.1, 1, 10, 100 and 1000 μg/0.1 ml
Controls
Untreated negative controls:
not specified
Negative solvent / vehicle controls:
yes
True negative controls:
not specified
Positive controls:
not specified

Results and discussion

Test results
Species / strain:
S. typhimurium, other: no strains are indicated
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
not specified
Untreated negative controls validity:
not specified
Positive controls validity:
not specified
Remarks on result:
other: other: not indicated
Remarks:
Migrated from field 'Test system'.

Any other information on results incl. tables

These tests permit the detection of point mutations in bacteria induced by chemical substances. Any mutagenic effects of the substances are demonstrable on comparison of the numbers of bacteria in  the treated and control cultures that have undergone back-mutation to histidine-prototrophism. To ensure that mutagenic effects of metabolites of the test substances formed in mammals would also be detected, experiments were performed in which the cultures were additionally treated with an activation mixture (rat liver microsomes and co-factors).

In the experiments performed with and without microsomal activation, comparison of the numbers of back-mutant colonies in the controls and the cultures treated with the various concentrations of the test substance revealed no significant deviations.

Thus, no evidence of the induction of point mutations by the test substance or by the metabolites of the substance formed as a result of microsomal activation was detectable in the strains of S. typhimurium used in these experiments.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative