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EC number: 259-910-3 | CAS number: 55934-93-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1988
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study was carried out under GLP and in conformity with the OECD guideline for Testing of Chemicals No.401: "Acute oral toxicity".
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 988
- Report date:
- 1987
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- [(butoxymethylethoxy)methylethoxy]propan-1-ol
- EC Number:
- 259-910-3
- EC Name:
- [(butoxymethylethoxy)methylethoxy]propan-1-ol
- Cas Number:
- 55934-93-5
- Molecular formula:
- C13H28O4
- IUPAC Name:
- 1-[2-(2-butoxy-1-methylethoxy)-1-methylethoxy]propan-1-ol
- Details on test material:
- - Name of test material (as cited in study report): Dowanol TPnB
-Chemical name: Tripropylene glycol n-butyl ether
- Physical state: Colourless liquid
- Analytical purity: 99%
- Lot/batch No.: May '87
- Specific gravity: 0.92
- Expiration date of radiochemical substance (if radiolabelling):
- Stability under test conditions: Estimated to be min. 2 years under these conditions
- Storage condition of test material: At ambient temperature in the dark
- Solubilityin water: 3%
- Vapor pressure : <0.01 mmHg/20°C
- Boiling point: >274°C
- Freezing point: <75°C
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River Wiga GmbH, SPF- quality, randomly bred
- Age at study initiation: 11 weeks old
- Fasting period before study: 3-4 hours
- Housing:Polycarbonate cages containing purified sawdust as bedding material.
- Diet (e.g. ad libitum): Ad libitum
- Water (e.g. ad libitum):Ad libitum
- Acclimation period:5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 19-23°C
- Humidity (%): 65-85°C
- Photoperiod (hrs dark / hrs light): 12 hrs dark/12 hrs/light
Pilot study
IN-LIFE DATES: From: 18-08-1987 To:25-08-1987
Main study
IN-LIFE DATES: From: 02-09-1987 To:16-09-1987
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- not specified
- Details on oral exposure:
- VEHICLE
MAXIMUM DOSE VOLUME APPLIED:
Dose range finding: 5000mg/kg
Main study: 4200 mg/kg
DOSAGE PREPARATION (if unusual): The dose volume (ml/kg body weight) was calculated as follows;
Dosis: g/kg body weight/specific gravity in (g/ml). The specific gravity used was 0.09284 g/ml.
CLASS METHOD (if applicable)
- Rationale for the selection of the starting dose: Based on the toxicity observed in the dose range finding investigation three groups of animals, each comprising 5 males and 5 females, were dosed with a single oral dose of the test substance at 2400, 3200 and 4200 mg/kg body weight, respectively. - Doses:
- single dose of 2400, 3200, and 4200 mg/kg bw
- No. of animals per sex per dose:
- 30
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Daily
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight,organ weights, histopathology. - Statistics:
- Established statistical procedure (e.g. Finney,1971)
Results and discussion
- Preliminary study:
- Three groups of animals, each comprising 1 male and 1 female, were dosed with a single oral dose of the test substance at 2500, 4000 and 5000 mg/kg body weight, respectively. Both animals of the 4000 mg/kg and 5000 mg/kg dose groups died with in 24 hours of dosing. Symptoms of toxicity were ataxia, lethargy, tremors, convulsions, unreactive, piloerection and abnormal respiration (i.e., dyspnoea, bradypnoea, rattled respiration). Animals of the low dose group showed as of day 3 no more signs of toxicity. The observation period was 7 days.
Effect levelsopen allclose all
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- ca. 2 800 mg/kg bw
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 3 100 mg/kg bw
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 2 600 mg/kg bw
- Mortality:
- The incidence of mortalities for the sexes combined from low to high dose group was 3, 6 and 10. All deaths occured within 24 hours of dosing.
- Clinical signs:
- other: Major signs of toxicity were lethargy, ataxia, convulsions, tremors and unreactiveness. For surviving animals these signs were reversible since as of day 2 no more significant abnormalities were observed during the 14-day observation period.
- Gross pathology:
- Macroscopic examination of animals at necropsy revealed bloody nose, bloody eye encrustation, moist eyes, red patches in the glandular part of the stomach and in the pancreas, petechiae of the stomach and the thymus, bloody contents or haemorrhage of the small intestine, haemothorax and dark red lungs.
Any other information on results incl. tables
The incidence of mortalities for the sexes combined from low to high dose group was 3, 6, and 10. All deaths occurred within 24 hours of dosing. Major signs of toxicity were lethargy, ataxia, convulsions, tremors and
unreactiveness. For surviving animals these signs were reversible since as of day 2 no more abnormalities were
observed during the 14-day observation period. Major macroscopic abnormalities of animals at necropsy were bloody nose, bloody eye encrustation, red patches in the glandular part of the stomach and in the
pancreas, petechiae of the stomach and
the thymus, bloody contents or haemorrhage of the small
intestine, haemothorax and dark red lungs.
The LD50-value of DOWANOL TPnB for the sexes combined
amounted to 2.8 g/kg body weight, whereas the LD50-values
for the sexes separately were 3.1 and 2.6 g/kg for males and
females, respectively.
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Remarks:
- Criteria used for interpretation of results: EU
- Conclusions:
- The LD50 value for the sexes combined amounted to 2.8 g/kg body weight, whereas the LD50 values for the sexes separately were 3.1 and 2.6 g/kg for males and females, respectively.
- Executive summary:
The purpose of the study was to obtain information on the toxicity of the test substance when administered to rats in a single oral dose. Furthermore, the study was intended to derive the LD50. The study was conducted according to OECD guideline 401.
Three groups of 5 male and 5 female, young adult rats of wistar strain were used to access the acute toxicity study of Tripropylene glycol n-butyl ether. Animals were fasted overnight prior to dosing until 3 -4 hours after administration of the test substance.The undiluted test substance was administered once only by gavage at a dose level of 2400, 3200 and 4200 mg/kg body weight respectively.
The incidence of mortalities for the sexes combined from low to high dose group was 3, 6 and 10. All deaths occurred within 24 hours of dosing. Major signs of toxicity were lethargy, ataxia, convulsions, tremors and unreactiveness. For surviving animals these signs were reversible since as of day 2. No more significant abnormalities were observed during the 14 day observation period. All surviving animals showed body weight gain during the study period.
Macroscopic examination of animals at necropsy revealed bloody nose, bloody eye encrustation, moist eyes, red patches in the glandular part of the stomach and in the pancreas, petechiae of the stomach and the thymus, bloody contents or haemorrhage of the small intestine, haemothorax and dark red lungs.
The LD50 value for the sexes combined was calculated to be approximately 2800 mg/kg body weight. The LD50 values for the sexes separately were 3100 and 2600 mg/kg for males and females, respectively.
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